公开(公告)号：US09409942B2

公开(公告)日：2016-08-09

申请号：US13951285

申请日：2013-07-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Deb Chatterjee ,  Mary Longo ,  Elizabeth Flynn ,  Robert Oberfelder

IPC: C07K9/00 ,  C07K1/26 ,  C07K1/107 ,  C07K1/13 ,  C12N9/02 ,  C12P21/02 ,  C07K14/00

CPC classification number: C07K1/26 ,  C07K1/1077 ,  C07K1/13 ,  C07K14/001 ,  C07K2319/00 ,  C07K2319/01 ,  C12N9/0036 ,  C12P21/02 ,  Y02P20/52

Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies thereby facilitating rapid isolation and purification of recombinant proteins. The invention further provides methods for producing protein molecular weight ladders for us in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

Abstract translation: 本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。 本发明的方法通过形成包含编码与包含配对蛋白的多肽有效连接的多肽的遗传序列的基因构建体进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体，从而促进快速分离和纯化 重组蛋白。 本发明还提供了在蛋白质凝胶电泳中为我们生产蛋白质分子量梯子的方法，以及通过这些方法制备的蛋白质和蛋白质分子量梯。

公开(公告)号：US20160024494A1

公开(公告)日：2016-01-28

申请号：US14875602

申请日：2015-10-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mekbib ASTATKE ,  Deb Chatterjee ,  Gary Gerard

IPC: C12N15/11 ,  A61K31/7052 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12N15/11 ,  A61K31/7052 ,  C12N9/1247 ,  C12N9/1252 ,  C12N9/1276 ,  C12N2310/122 ,  C12N2310/315 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Y207/07006 ,  C12Y207/07007 ,  C12Y207/07049 ,  C12Q2527/125

Abstract: The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.

Abstract translation: 本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。 在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。 具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。 核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。 因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。 因此，本发明防止，减少或显着降低非特异性核酸合成。 本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。 本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。 因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。 因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。