公开(公告)号：US20140097086A1

公开(公告)日：2014-04-10

申请号：US13951285

申请日：2013-07-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Deb K. CHATTERJEE ,  Mary Longo ,  Elizabeth Flynn ,  Robert Oberfelder

IPC: C07K1/26 ,  C07K14/00

CPC classification number: C07K1/26 ,  C07K1/1077 ,  C07K1/13 ,  C07K14/001 ,  C07K2319/00 ,  C07K2319/01 ,  C12N9/0036 ,  C12P21/02 ,  Y02P20/52

Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies thereby facilitating rapid isolation and purification of recombinant proteins. The invention further provides methods for producing protein molecular weight ladders for us in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

Abstract translation: 本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。 本发明的方法通过形成包含编码与包含配对蛋白的多肽有效连接的多肽的遗传序列的基因构建体进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体，从而促进快速分离和纯化 重组蛋白。 本发明还提供了在蛋白质凝胶电泳中为我们生产蛋白质分子量梯子的方法，以及通过这些方法制备的蛋白质和蛋白质分子量梯。

公开(公告)号：US09409942B2

公开(公告)日：2016-08-09

申请号：US13951285

申请日：2013-07-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Deb Chatterjee ,  Mary Longo ,  Elizabeth Flynn ,  Robert Oberfelder

IPC: C07K9/00 ,  C07K1/26 ,  C07K1/107 ,  C07K1/13 ,  C12N9/02 ,  C12P21/02 ,  C07K14/00

CPC classification number: C07K1/26 ,  C07K1/1077 ,  C07K1/13 ,  C07K14/001 ,  C07K2319/00 ,  C07K2319/01 ,  C12N9/0036 ,  C12P21/02 ,  Y02P20/52

Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies thereby facilitating rapid isolation and purification of recombinant proteins. The invention further provides methods for producing protein molecular weight ladders for us in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

Abstract translation: 本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。 本发明的方法通过形成包含编码与包含配对蛋白的多肽有效连接的多肽的遗传序列的基因构建体进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体，从而促进快速分离和纯化 重组蛋白。 本发明还提供了在蛋白质凝胶电泳中为我们生产蛋白质分子量梯子的方法，以及通过这些方法制备的蛋白质和蛋白质分子量梯。