公开(公告)号：US20170157538A1

公开(公告)日：2017-06-08

申请号：US15375271

申请日：2016-12-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian Reed ,  JAMES A. BALL

IPC: B01D17/04 ,  C07H21/00 ,  C07H1/06

CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.