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公开(公告)号:US11578360B2
公开(公告)日:2023-02-14
申请号:US16949083
申请日:2020-10-13
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin Li , Kai Qin Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68 , C12Q1/6853 , C12Q1/6844 , C12Q1/6806 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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2.Methods, compositions, systems, apparatuses and kits for nucleic acid amplification 有权
Title translation: 用于核酸扩增的方法,组合物,系统,装置和试剂盒公开(公告)号:US09309566B2
公开(公告)日:2016-04-12
申请号:US13828049
申请日:2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin Li , Kai Qin Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68
CPC classification number: C12Q1/6853 , C12Q1/6874 , C12Q2521/531 , C12Q2525/117 , C12Q2535/119 , C12Q2523/313
Abstract: In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.
Abstract translation: 在一些实施方案中,本教导提供了用于配对末端测序的方法。 在一些实施方案中,待进行配对末端测序的多核苷酸模板包含至少一个交联部分和至少一个可剪切部分。 在一些实施方案中,配对末端测序反应包括(a)正向测序步骤,(b)切割步骤,和(c)反向测序步骤。 在一些实施方案中,配对末端测序反应包括(a)正向测序步骤,(b)交联步骤,(c)切割步骤和(d)反向测序步骤。
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公开(公告)号:US10913976B2
公开(公告)日:2021-02-09
申请号:US16351194
申请日:2019-03-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin Li , Kai Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68 , C12Q1/6853 , C12Q1/6844 , C12Q1/6806 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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公开(公告)号:US10233488B2
公开(公告)日:2019-03-19
申请号:US15276713
申请日:2016-09-26
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin Li , Kai Qin Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68 , C12Q1/6853 , C12Q1/6844 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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5.Clonal amplification of nucleic acid on solid surface with template walking 有权
Title translation: 用模板步行在固体表面上克隆扩增核酸公开(公告)号:US09476080B2
公开(公告)日:2016-10-25
申请号:US14692706
申请日:2015-04-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin Li , Kai Qin Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
CPC classification number: C12Q1/46 , C12P19/34 , C12Q1/6834 , C12Q1/6846 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2531/119 , C12Q2565/537
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
Abstract translation: 提供了在载体上产生固定化克隆扩增子的局部群体的新方法。
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