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    Nucleic acid amplification
    1.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US10329544B2

    公开(公告)日：2019-06-25

    申请号：US15091717

    申请日：2016-04-06

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao ,  Wolfgang Hinz

    IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Nucleic acid amplification
    2.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US10113195B2

    公开(公告)日：2018-10-30

    申请号：US14789922

    申请日：2015-07-01

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6874

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Nucleic acid adaptors and uses thereof
    7.
    发明授权
    Nucleic acid adaptors and uses thereof 有权

    公开(公告)号：US10450608B2

    公开(公告)日：2019-10-22

    申请号：US15372938

    申请日：2016-12-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Bin Li ,  Zhoutao Chen ,  Tanya Biorac ,  Melvin Wei

    IPC: C12P19/34 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12N15/10 ,  C12N9/12 ,  C12N9/20 ,  C12Q1/6832 ,  C12N15/64

    Abstract: Provided herein are compositions, systems, methods, and kits for joining together the ends of one or more polynucleotides using at least one pair of blocking oligonucleotide adaptors. Blocking oligonucleotide adaptors can comprise a double-stranded oligonucleotide adaptor (duplex) having an overhang cohesive portion that anneals with a blocking oligonucleotide which can be a separate single-stranded oligonucleotide. Also provided herein are compositions generated using the blocking oligonucleotide adapters, including circularized polynucleotides, that may be manipulated, for example, through nick translation, to yield a linear mate pair construct.

    Nucleic acid amplification
    8.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US09334531B2

    公开(公告)日：2016-05-10

    申请号：US14023361

    申请日：2013-09-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC: C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Y207/07007 ,  C12Q2531/119 ,  C12Q2565/537

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Nucleic acid amplification
    9.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US11001815B2

    公开(公告)日：2021-05-11

    申请号：US16442341

    申请日：2019-06-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    Nucleic acid amplification
    10.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US10858695B2

    公开(公告)日：2020-12-08

    申请号：US16168810

    申请日：2018-10-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan M. Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6853

    Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

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