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公开(公告)号：US09476093B2
公开(公告)日：2016-10-25
申请号：US14593858
申请日：2015-01-09
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Lei Xi , Paul Kenney , Zhaochun Ma , Dennis Prosen , Stephen Hendricks , Roland Nagel
CPC classification number: C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.
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公开(公告)号：US11578360B2
公开(公告)日：2023-02-14
申请号：US16949083
申请日：2020-10-13
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Bin Li , Kai Qin Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68 , C12Q1/6853 , C12Q1/6844 , C12Q1/6806 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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3.发明授权Methods, compositions, systems, apparatuses and kits for nucleic acid amplification 有权用于核酸扩增的方法，组合物，系统，装置和试剂盒公开(公告)号：US09309566B2
公开(公告)日：2016-04-12
申请号：US13828049
申请日：2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Bin Li , Kai Qin Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68
CPC classification number: C12Q1/6853 , C12Q1/6874 , C12Q2521/531 , C12Q2525/117 , C12Q2535/119 , C12Q2523/313
Abstract: In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.
Abstract translation: 在一些实施方案中，本教导提供了用于配对末端测序的方法。 在一些实施方案中，待进行配对末端测序的多核苷酸模板包含至少一个交联部分和至少一个可剪切部分。 在一些实施方案中，配对末端测序反应包括（a）正向测序步骤，（b）切割步骤，和（c）反向测序步骤。 在一些实施方案中，配对末端测序反应包括（a）正向测序步骤，（b）交联步骤，（c）切割步骤和（d）反向测序步骤。
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4.发明申请公开(公告)号：US20170305954A1
公开(公告)日：2017-10-26
申请号：US15643053
申请日：2017-07-06
Applicant: Life Technologies Corporation
Inventor： Zhaochun Ma , Khairuzzaman Bashar Mullah , Robert G. Eason
IPC: C07H19/16 , C07H21/04 , C07H21/02 , C07H19/06 , C07D239/54 , C07D473/34 , C07D473/18 , C07D405/04 , C12Q1/68 , C07H21/00
CPC classification number: C07H19/16 , C07D239/54 , C07D405/04 , C07D473/18 , C07D473/34 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6806
Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
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5.发明授权Synthesis of 2′, 3′-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization 有权用于自动DNA合成和焦磷酸裂解活化聚合的2'，3'-双脱氧核苷合成公开(公告)号：US09228227B2
公开(公告)日：2016-01-05
申请号：US14173725
申请日：2014-02-05
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Zhaochun Ma , Khairuzzaman Bashar Mullah , Robert Eason
IPC: C07D473/18 , C12Q1/68 , C07D405/04 , C07D473/34 , C07H19/16 , C07D239/54 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04
CPC classification number: C07H19/16 , C07D239/54 , C07D405/04 , C07D473/18 , C07D473/34 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6806
Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
Abstract translation: 公开了制备2'，3'-二脱氧核苷酸支持结构的方法，例如2'，3'-二脱氧鸟苷，2'，3'-二脱氧腺苷和3'-脱氧胸苷支持结构。 还提供了使用这些结构的各种方法，例如其用于自动DNA合成和焦磷酸裂解活化聚合的用途。
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Patent Agency Ranking
6.发明申请POLYMERIZATION OF NUCLEIC ACIDS USING ACTIVATION BY POLYPHOSPHOROLYSIS (APP) REACTIONS 有权使用多磷酸（APP）反应活化的核酸的聚合公开(公告)号：US20150191775A1
公开(公告)日：2015-07-09
申请号：US14593858
申请日：2015-01-09
Applicant: Life Technologies Corporation
Inventor： Lei XI , Paul Kenney , Zhaochun Ma , Dennis Prosen , Stephen Hendricks , Roland Nagel
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.
Abstract translation: 本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代磷酸盐（或μ-一硫代焦磷酸盐）和相关化合物中的至少一种进行多磷酸解（APP）反应进行活化的方法。
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公开(公告)号：US10913976B2
公开(公告)日：2021-02-09
申请号：US16351194
申请日：2019-03-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Bin Li , Kai Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68 , C12Q1/6853 , C12Q1/6844 , C12Q1/6806 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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公开(公告)号：US10233488B2
公开(公告)日：2019-03-19
申请号：US15276713
申请日：2016-09-26
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Bin Li , Kai Qin Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68 , C12Q1/6853 , C12Q1/6844 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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公开(公告)号：US09732112B2
公开(公告)日：2017-08-15
申请号：US14952149
申请日：2015-11-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Zhaochun Ma , Khairuzzaman Bashar Mullah , Robert G. Eason
IPC: C07H19/06 , C07H19/16 , C07D473/34 , C07D405/04 , C07D473/18 , C07D239/54 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/68
CPC classification number: C07H19/16 , C07D239/54 , C07D405/04 , C07D473/18 , C07D473/34 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6806
Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
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10.发明授权公开(公告)号：US09476080B2
公开(公告)日：2016-10-25
申请号：US14692706
申请日：2015-04-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Bin Li , Kai Qin Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
CPC classification number: C12Q1/46 , C12P19/34 , C12Q1/6834 , C12Q1/6846 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2531/119 , C12Q2565/537
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
Abstract translation: 提供了在载体上产生固定化克隆扩增子的局部群体的新方法。
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