公开(公告)号：US20140134624A1

公开(公告)日：2014-05-15

申请号：US13975056

申请日：2013-08-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David RUFF ,  Mark Shannon ,  Kenneth Livak ,  Karl Guegler ,  Kevin Hennessy

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C07K14/47 ,  C07K14/4711 ,  C12Q1/6816 ,  C12Q2533/107

Abstract: The present teachings provide methods, compositions, and kits for detecting the presence of protein aggregates. In some embodiments, the protein aggregate is treated with a labeled precursor, and the labeled precursor is incorporated into the protein aggregate to form a labeled protein aggregate. The labeled protein aggregate is then measured, thus detecting the presence of the protein aggregate. In some embodiments, the labeled protein aggregate is detected by interaction of labeled precursors, for example by a proximity ligation assay.

Abstract translation: 本教导提供了用于检测蛋白质聚集体的存在的方法，组合物和试剂盒。 在一些实施方案中，用标记的前体处理蛋白质聚集体，并将标记的前体掺入蛋白质聚集体中以形成标记的蛋白质聚集体。 然后测量标记的蛋白质聚集体，从而检测蛋白质聚集体的存在。 在一些实施方案中，标记的蛋白质聚集体通过标记的前体的相互作用来检测，例如通过邻近连接测定。