公开(公告)号：US20130210674A1

公开(公告)日：2013-08-15

申请号：US13743217

申请日：2013-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ian A. HARDING ,  Donald SANDELL ,  Kevin BODNER ,  Jaynish PATEL ,  Timothy WOUDENBERG ,  Mark OLDHAM ,  H. Pin KAO ,  Gary LIM ,  John P. BODEAU ,  Dar BAHATT ,  Sergey V. ERMAKOV ,  Adrian FAWCETT ,  Jones C. Jones

IPC: C12N15/10

CPC classification number: C12N15/1072 ,  B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2300/021 ,  B01L2300/041 ,  B01L2300/0829 ,  B01L2300/12 ,  B01L2300/1805

Abstract: A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.

公开(公告)号：US20160047747A1

公开(公告)日：2016-02-18

申请号：US14779532

申请日：2014-04-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael M. LAFFERTY ,  Jonathan M. ROTHBERG ,  Keith G. FIFE ,  Mark OLDHAM ,  Eric NORDMAN

IPC: G01N21/64

CPC classification number: G01N21/648 ,  C12Q1/6874 ,  G01N21/6454 ,  G01N2021/6441 ,  C12Q2563/107 ,  C12Q2563/155 ,  C12Q2565/101 ,  C12Q2565/629 ,  C12Q2565/632 ,  C12Q2565/607

Abstract: A device including a transparent layer defining a surface exposed to a flow volume and to secure a target polynucleotide template and a detector structure secured to the transparent layer and including a plurality of detectors to detect a signal emitted during nucleotide incorporation along the target polynucleotide template.

Abstract translation: 包括限定暴露于流量的表面的透明层并且固定目标多核苷酸模板和固定到透明层的检测器结构并包括多个检测器以检测沿着靶多核苷酸模板的核苷酸掺入期间发射的信号的透明层的装置。