公开(公告)号：US20170145214A1

公开(公告)日：2017-05-25

申请号：US15423890

申请日：2017-02-03

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Kyle GEE ,  Upinder SINGH ,  Aleksey RUKAVISHNIKOV ,  Daniel BEACHAM ,  Shih-Jung HUANG ,  Michael JANES ,  Wenjun ZHOU

IPC分类号： C09B11/24 ,  G01N33/84 ,  G01N33/58

CPC分类号： C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

摘要： Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

公开(公告)号：US20160334393A1

公开(公告)日：2016-11-17

申请号：US15221230

申请日：2016-07-27

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Kyle GEE ,  Michael JANES ,  Iain JOHNSON ,  Hee Chol KANG

IPC分类号： G01N33/52 ,  G01N33/58 ,  C07F9/54 ,  C07D491/16 ,  C07K5/02

CPC分类号： G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

摘要： Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.