公开(公告)号：US20250019756A1

公开(公告)日：2025-01-16

申请号：US18739037

申请日：2024-06-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda LEE ,  Sam WOO ,  Peter MA

IPC: C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

公开(公告)号：US20140099645A1

公开(公告)日：2014-04-10

申请号：US14053571

申请日：2013-10-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda LEE ,  Sam WOO ,  Peter MA

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Abstract translation: 提供了一种组合物，其包含带负电荷的基团，寡核苷酸序列和至少一个或一个核酸酶抗性连锁基团以形成化学增强的引物。 化学增强引物可用于测序和片段分析。 还提供了合成引物的方法以及用于测序的DNA的制备方法和测序DNA的方法以及含有化学增强的引物的试剂盒。 DNA测序的方法可以包括使扩增反应产物与组合物在过量扩增引物被核酸酶降解的条件下接触，化学增强引物基本上不降解。

公开(公告)号：US20170051342A1

公开(公告)日：2017-02-23

申请号：US15230834

申请日：2016-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LINDA LEE ,  Sam WOO ,  Peter MA

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Abstract translation: 提供了一种组合物，其包含带负电荷的基团，寡核苷酸序列和至少一个或一个核酸酶抗性连锁基团以形成化学增强的引物。 化学增强引物可用于测序和片段分析。 还提供了合成引物的方法以及用于测序的DNA的制备方法和测序DNA的方法以及含有化学增强的引物的试剂盒。 DNA测序的方法可以包括使扩增反应产物与组合物在过量扩增引物被核酸酶降解的条件下接触，化学增强引物基本上不降解。

公开(公告)号：US20210269871A1

公开(公告)日：2021-09-02

申请号：US17193232

申请日：2021-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Swati GOYAL ,  Achim KARGER ,  Peter MA ,  S. Jeffrey ROSNER ,  Ian WALTON ,  Jonathan WANG ,  Michael WENZ

IPC: C12Q1/6869 ,  B01L3/00 ,  C12Q1/6806

Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.