RECOMBINANT REVERSE TRANSCRIPTASES
    2.
    发明申请
    RECOMBINANT REVERSE TRANSCRIPTASES 有权
    重组逆转录

    公开(公告)号:US20130189758A1

    公开(公告)日:2013-07-25

    申请号:US13730108

    申请日:2012-12-28

    CPC classification number: C12N9/1276 C12Y207/07049

    Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

    Abstract translation: 本发明涉及编码具有DNA聚合酶活性和显着降低的RNA酶H活性的多反应逆转录酶的基因,含有本发明转化的基因和宿主细胞的载体。 本发明还包括使用本发明的逆转录酶产生过度逆转录酶,从mRNA产生cDNA的方法,使用过度反转录酶制备的试剂盒和测定模板。

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