公开(公告)号：US20150361481A1

公开(公告)日：2015-12-17

申请号：US14736509

申请日：2015-06-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191

Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散载体的单个反应混合物和多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 靶核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和第二靶核酸群，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合到第二群珠中的珠粒。 单个反应混合物可以用于单层扩增第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

公开(公告)号：US20240102079A1

公开(公告)日：2024-03-28

申请号：US18241617

申请日：2023-09-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

IPC: C12Q1/6806 ,  C12Q1/6844

CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6846

Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

公开(公告)号：US20190338343A1

公开(公告)日：2019-11-07

申请号：US16514814

申请日：2019-07-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

IPC: C12Q1/6806 ,  C12Q1/6844

Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.