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    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    1.
    发明公开
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20240102079A1

    公开(公告)日：2024-03-28

    申请号：US18241617

    申请日：2023-09-01

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/6806 ,  C12Q1/6844

    CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6846

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    METHODS AND SYSTEMS FOR POINT OF USE REMOVAL OF SACRIFICIAL MATERIAL
    3.
    发明申请
    METHODS AND SYSTEMS FOR POINT OF USE REMOVAL OF SACRIFICIAL MATERIAL 有权
    使用移除材料的方法和系统

    公开(公告)号：US20140191292A1

    公开(公告)日：2014-07-10

    申请号：US13734696

    申请日：2013-01-04

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Christina INMAN ,  Alexander MASTROIANNI ,  Wolfgang HINZ ,  Shifeng LI ,  Scott BENSON

    IPC: H01L27/105 ,  H01L21/8234

    CPC classification number: H01L21/31105 ,  G01N27/4145 ,  H01L21/31144

    Abstract: A method of manufacturing a sensor, the method including forming an array of chemically-sensitive field effect transistors (chemFETs), depositing a dielectric layer over the chemFETs in the array, depositing a protective layer over the dielectric layer, etching the dielectric layer and the protective layer to form cavities corresponding to sensing surfaces of the chemFETs, and removing the protective layer. The method further includes, etching the dielectric layer and the protective layer together to form cavities corresponding to sensing surfaces of the chemFETs. The protective layer is at least one of a polymer, photoresist material, noble metal, copper oxide, and zinc oxide. The protective layer is removed using at least one of sodium hydroxide, organic solvent, aqua regia, ammonium carbonate, hydrochloric acid, acetic acid, and phosphoric acid.

    Abstract translation: 一种制造传感器的方法，所述方法包括形成化学敏感场效应晶体管阵列（chemFET），在阵列中的chemFET上沉积电介质层，在电介质层上沉积保护层，蚀刻电介质层和 保护层以形成对应于chemFET的感测表面的空腔，以及去除保护层。 该方法还包括：将电介质层和保护层一起蚀刻以形成对应于chemFET的感测表面的空腔。 保护层是聚合物，光致抗蚀剂材料，贵金属，氧化铜和氧化锌中的至少一种。 使用氢氧化钠，有机溶剂，王水，碳酸铵，盐酸，乙酸和磷酸中的至少一种除去保护层。

    Asymmetric Rhodamine Dye and Use Thereof in Biological Assays
    4.
    发明申请
    Asymmetric Rhodamine Dye and Use Thereof in Biological Assays 有权

    公开(公告)号：US20220380845A1

    公开(公告)日：2022-12-01

    申请号：US17299671

    申请日：2019-12-20

    Applicant: Life Technologies Corporation

    Inventor： Brian EVANS ,  Scott BENSON

    IPC: C12Q1/6876 ,  C09B11/24 ,  C07H21/00

    Abstract: The present disclosure relates to N-protected NH-rhodamine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays. Those fluorescent compounds are useful to label synthetic oligonucleotides. Formula (I).

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    5.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开
    多功能核酸扩增

    公开(公告)号：US20150361481A1

    公开(公告)日：2015-12-17

    申请号：US14736509

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散载体的单个反应混合物和多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 靶核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和第二靶核酸群，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合到第二群珠中的珠粒。 单个反应混合物可以用于单层扩增第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    8.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190338343A1

    公开(公告)日：2019-11-07

    申请号：US16514814

    申请日：2019-07-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/6806 ,  C12Q1/6844

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

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