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    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    1.
    发明公开
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20240102079A1

    公开(公告)日：2024-03-28

    申请号：US18241617

    申请日：2023-09-01

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/6806 ,  C12Q1/6844

    CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6846

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    SYSTEM AND METHOD FOR EMULSION BREAKING
    2.
    发明申请
    SYSTEM AND METHOD FOR EMULSION BREAKING 审中-公开

    公开(公告)号：US20200282336A1

    公开(公告)日：2020-09-10

    申请号：US16786913

    申请日：2020-02-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brian REED ,  James A. BALL

    IPC: B01D17/04 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806

    Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    4.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190338343A1

    公开(公告)日：2019-11-07

    申请号：US16514814

    申请日：2019-07-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/6806 ,  C12Q1/6844

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    6.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开
    多功能核酸扩增

    公开(公告)号：US20150361481A1

    公开(公告)日：2015-12-17

    申请号：US14736509

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散载体的单个反应混合物和多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 靶核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和第二靶核酸群，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合到第二群珠中的珠粒。 单个反应混合物可以用于单层扩增第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

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