公开(公告)号：US20180237826A1

公开(公告)日：2018-08-23

申请号：US15957128

申请日：2018-04-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark ANDERSEN ,  Steven ROMAN

IPC: C12Q1/6806 ,  C07H21/00 ,  C12Q1/6853

CPC classification number: C12Q1/6806 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2563/131 ,  C12Q2563/149

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

公开(公告)号：US20200239936A1

公开(公告)日：2020-07-30

申请号：US16846932

申请日：2020-04-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark ANDERSEN ,  Steven ROMAN

IPC: C12Q1/6806 ,  C12Q1/6853 ,  C07H21/00

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

公开(公告)号：US20150353993A1

公开(公告)日：2015-12-10

申请号：US14829297

申请日：2015-08-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark ANDERSEN ,  Steven ROMAN

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2563/131 ,  C12Q2563/149

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

Abstract translation: 本发明提供了可用于从样品中分离核酸分子的方法，组合物，试剂盒，系统和装置。 特别地，所述方法通常涉及从样品归一化靶核酸分子的浓度。 一方面，本发明涉及从引物延伸反应混合物中纯化引物延伸产物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的核酸分子可以用于包括核酸测序在内的各种下游过程。

公开(公告)号：US20140120529A1

公开(公告)日：2014-05-01

申请号：US14054618

申请日：2013-10-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark ANDERSEN ,  Steven ROMAN

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2563/131 ,  C12Q2563/149

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

Abstract translation: 本发明提供了可用于从样品中分离核酸分子的方法，组合物，试剂盒，系统和装置。 特别地，所述方法通常涉及从样品归一化靶核酸分子的浓度。 一方面，本发明涉及从引物延伸反应混合物中纯化引物延伸产物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的核酸分子可以用于包括核酸测序在内的各种下游过程。