公开(公告)号：US20200072826A1

公开(公告)日：2020-03-05

申请号：US16543389

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Hua YU ,  Xiaoling YANG ,  Rui ZHENG ,  Chiu Tai Andrew WONG ,  Jeremy GRAY

IPC: G01N33/543 ,  C12Q1/6844

Abstract: The disclosure generally relates to systems, methods, and apparatuses for magnetic bead loading. An example embodiment of the disclosure relates to mixing magnetic beads with sequencing beads to form a solution. The solution containing both beads is injected onto a microchip having a plurality of microwells. The magnetic beads may have larger diameter than the microwell while the sequencing beads may have a smaller diameter, allowing them to enter and reside in the microwell. One or more magnets positioned under the microchip move back and forth across the microchip surface. The magnetic beads form a line and follow the movement of the magnets. During rounds of sweeping, the sequencing beads load into the respective wells. The magnets may be disengaged and the magnetic beads may be washed away after the sequencing beads are loaded.