公开(公告)号：US20190071723A1

公开(公告)日：2019-03-07

申请号：US16182384

申请日：2018-11-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Phil WAGGONER ,  James A. BALL ,  Wolfgang HINZ ,  Michael L. MINTO ,  Scott PARKER ,  David M. COX ,  Alexander MASTROIANNI ,  Jeremy GRAY ,  Marc GLAZER ,  Kimberly GORRELL

IPC: C12Q1/6869 ,  G01N27/414

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

公开(公告)号：US20230175046A1

公开(公告)日：2023-06-08

申请号：US18104599

申请日：2023-02-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Wolfgang HINZ ,  Ronald CICERO ,  Christina INMAN ,  Paul KENNEY ,  Alexander MASTROIANNI ,  Roman ROZHKOV ,  Yufang WANG ,  Jeremy GRAY ,  Marc GLAZER ,  Dmitriy GREMYACHINSKIY

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

CPC classification number: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US20220220534A1

公开(公告)日：2022-07-14

申请号：US17708813

申请日：2022-03-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham ROSENBAUM ,  Collyn SEEGER ,  Jeremy GRAY ,  Hua YU

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US20250043342A1

公开(公告)日：2025-02-06

申请号：US18920456

申请日：2024-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Philip WAGGONER ,  James A. BALL ,  Michael L. MINTO ,  David M. COX ,  Wolfgang HINZ ,  Alexander MASTROIANNI ,  Jeremy GRAY ,  Marc GLAZER ,  Scott PARKER ,  Kimberly GORRELL

IPC: C12Q1/6869 ,  G01N27/414

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

公开(公告)号：US20240309443A1

公开(公告)日：2024-09-19

申请号：US18407234

申请日：2024-01-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Philip WAGGONER ,  James A. BALL ,  Michael L. MINTO ,  David M. COX ,  Wolfgang HINZ ,  Alexander MASTROIANNI ,  Jeremy GRAY ,  Marc GLAZER ,  Scott PARKER ,  Kimberly GORRELL

IPC: C12Q1/6869 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  G01N27/4145

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

公开(公告)号：US20160258011A1

公开(公告)日：2016-09-08

申请号：US15061273

申请日：2016-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Phil WAGGONER ,  James A. BALL ,  Wolfgang HINZ ,  Michael L. MINTO ,  Scott PARKER ,  David M. COX ,  Alexander MASTROIANNI ,  Jeremy GRAY ,  Marc GLAZER ,  Kimberly GORRELL

IPC: C12Q1/68 ,  G01N27/414

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Abstract translation: 传感器装置包括基板，设置在基板上的半导体器件，具有表面电极结构的半导体器件和形成在表面电极结构上的糖类涂层。 在使用前可以除去糖衣。 该半导体器件还可以包括一个阱以及可选地布置在阱中的珠。

公开(公告)号：US20160003768A1

公开(公告)日：2016-01-07

申请号：US14789795

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald L. CICERO ,  Marc GLAZER ,  Yufang WANG ,  Christina E. INMAN ,  Jeremy GRAY ,  Joseph KOSCINSKI ,  James A. BALL ,  Phil WAGGONER ,  Alexander MASTROIANNI

IPC: G01N27/414

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Abstract translation: 传感器部件包括传感器，传感器包括与传感器协作的传感器表面和反应部位，并暴露传感器表面。 反应位点包括反应位点表面。 表面剂与反应位点表面或传感器表面结合。 表面活性剂包括在反应位点表面或传感器表面上与布朗斯台德碱或路易斯酸官能团反应的表面活性官能团，并且包括不具有供体电子对的远端官能团。

公开(公告)号：US20160001249A1

公开(公告)日：2016-01-07

申请号：US14789650

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Ronald L. CICERO ,  Yufang WANG ,  Alexander MASTROIANNI ,  Prasanna Krishnan THWAR ,  Jeremy GRAY ,  Marc GLAZER ,  Tommie Lloyd LINCECUM, JR. ,  Mohammad ALANJARY ,  Joseph KOSCINSKI

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00677 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2523/32 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/155 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.

Abstract translation: 在传感器基板上加载珠粒的方法包括将包含珠粒的悬浮液施加到在传感器基底上限定的流动池中。 传感器基板包括多个孔。 珠粒至少部分沉积到多个孔中。 该方法还包括从流动池中去除液体，从流动池中蒸发液体，例如通过抽吸空气流过流动池; 以及将水合溶液施加到所述流动池。

公开(公告)号：US20240125733A1

公开(公告)日：2024-04-18

申请号：US18475640

申请日：2023-09-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald L. CICERO ,  James A. BALL ,  Alexander MASTROIANN1 ,  Christina E. INMAN ,  Jeremy GRAY ,  Marc GLAZER ,  Yufang WANG ,  Joseph KOSCINSKI ,  Phil WAGGONER

IPC: G01N27/414

CPC classification number: G01N27/4145

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

公开(公告)号：US20210102917A1

公开(公告)日：2021-04-08

申请号：US17088347

申请日：2020-11-03

Applicant: Life Technologies Corporation

Inventor： Jeremy GRAY ,  Jordan OWENS ,  James BUSTILLO

IPC: G01N27/414 ,  H01L29/423

Abstract: An apparatus includes a substrate; a gate structure disposed over the substrate and having an upper surface; a well structure disposed over the substrate and defining a well over the upper surface of the gate structure; a conductive layer disposed on the upper surface of the gate structure and at least partially extending along a wall of the well in the well structure; and a dielectric structure disposed over the well structure and defining an opening to the well.