公开(公告)号：US07888075B2

公开(公告)日：2011-02-15

申请号：US12177075

申请日：2008-07-21

申请人： Larry McCarthy ,  Lilly Kong ,  Michelle Tabb ,  Ming-Chou Lee

发明人： Larry McCarthy ,  Lilly Kong ,  Michelle Tabb ,  Ming-Chou Lee

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： Disclosed are methods of identifying a methicillin-resistant Staphylococcus aureus (MRSA) or methicillin-sensitive Staphylococcus aureus (MSSA) in a sample. The present invention provides a diagnostic method comprising modification of sequences of S. aureus by converting non-methylated cytosine residues ultimately into thymidine residues in the target nucleic acid. The invention further provides for the detection of modified sequences derived from the spa gene, the mecA gene, and the integrated SCCmec cassette of S. aureus.

摘要翻译： 公开了在样品中鉴定耐甲氧西林金黄色葡萄球菌（MRSA）或甲氧西林敏感性金黄色葡萄球菌（MSSA）的方法。 本发明提供了一种诊断方法，其包括通过将非甲基化胞嘧啶残基最终转移到靶核酸中的胸苷残基来修饰金黄色葡萄球菌的序列。 本发明进一步提供了检测源自温泉基因，mecA基因和金黄色葡萄球菌综合SCCmec盒的修饰序列。

公开(公告)号：US20090035780A1

公开(公告)日：2009-02-05

申请号：US12177075

申请日：2008-07-21

申请人： Larry McCarthy ,  Lilly Kong ,  Michelle Tabb ,  Ming-Chou Lee

发明人： Larry McCarthy ,  Lilly Kong ,  Michelle Tabb ,  Ming-Chou Lee

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： Disclosed are methods of identifying a methicillin-resistant Staphylococcus aureus (MRSA) or methicillin-sensitive Staphylococcus aureus (MSSA) in a sample. The present invention provides a diagnostic method comprising modification of sequences of S. aureus by converting non-methylated cytosine residues ultimately into thymidine residues in the target nucleic acid. The invention further provides for the detection of modified sequences derived from the spa gene, the mecA gene, and the integrated SCCmec cassette of S. aureus.

摘要翻译： 公开了在样品中鉴定耐甲氧西林金黄色葡萄球菌（MRSA）或甲氧西林敏感性金黄色葡萄球菌（MSSA）的方法。 本发明提供了一种诊断方法，其包括通过将非甲基化胞嘧啶残基最终转移到靶核酸中的胸苷残基来修饰金黄色葡萄球菌的序列。 本发明进一步提供了检测源自温泉基因，mecA基因和金黄色葡萄球菌综合SCCmec盒的修饰序列。

公开(公告)号：US20190211408A1

公开(公告)日：2019-07-11

申请号：US16306203

申请日：2017-06-02

申请人： Peter Lee ,  Raymond Huang ,  Kristin Ramos ,  Jules Chen ,  Michelle Tabb ,  Quest Diagnostics Investments LLC

发明人： Peter Lee ,  Raymond Huang ,  Kristin Ramos ,  Jules Chen ,  Michelle Tabb

IPC分类号： C12Q1/70

摘要： The present disclosure provides methods and compositions for determining whether a patient exhibiting acute gastroenteritis will benefit from treatment with therapeutic agents that inhibit Norovirus genogroup I (GI) or Norovirus genogroup II (GII). The methods disclosed herein are based on detecting Norovirus genogroup I (GI) and Norovirus genogroup II (GII) in a stool sample without extracting viral nucleic acids from a clinical specimen prior to performing real-time reverse transcription PCR. Kits for use in practicing the methods are also provided.

公开(公告)号：US09168523B2

公开(公告)日：2015-10-27

申请号：US13474903

申请日：2012-05-18

申请人： Peter D. Ludowise ,  David A. Whitman ,  Kyle C. Armantrout ,  Maurice Exner ,  Lucien A. E. Jacky ,  Michelle Tabb

发明人： Peter D. Ludowise ,  David A. Whitman ,  Kyle C. Armantrout ,  Maurice Exner ,  Lucien A. E. Jacky ,  Michelle Tabb

IPC分类号： G01N21/00 ,  B01L3/00

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/0605 ,  B01L2300/087 ,  B01L2400/0409 ,  G01N21/6486 ,  G01N35/025 ,  G01N35/04 ,  G01N2035/0441

摘要： Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material.

摘要翻译： 用于处理样品处理装置的系统和方法。 该系统可以包括样品处理装置，该样品处理装置包括检测室，被配置为围绕旋转轴线旋转样品处理装置的马达和相对于样品处理装置可操作地定位的光学模块，并配置成确定所选择的材料体积 存在于样品处理装置的检测室中。 该方法可以包括围绕旋转轴旋转样品处理装置，并且在旋转样品处理装置的同时确定所选择的材料体积是否存在于检测室中。 在一些实施例中，确定所选择的材料体积是否存在可以通过光学询问该检测室以获得该材料的光学特性。

公开(公告)号：US20120293796A1

公开(公告)日：2012-11-22

申请号：US13474903

申请日：2012-05-18

申请人： Peter D. Ludowise ,  David A. Whitman ,  Kyle C. Armantrout ,  Maurice Exner ,  Lucien A.E. Jacky ,  Michelle Tabb

发明人： Peter D. Ludowise ,  David A. Whitman ,  Kyle C. Armantrout ,  Maurice Exner ,  Lucien A.E. Jacky ,  Michelle Tabb

IPC分类号： G01N21/01

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/0605 ,  B01L2300/087 ,  B01L2400/0409 ,  G01N21/6486 ,  G01N35/025 ,  G01N35/04 ,  G01N2035/0441

摘要： Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material.

摘要翻译： 用于处理样品处理装置的系统和方法。 该系统可以包括样品处理装置，该样品处理装置包括检测室，被配置为围绕旋转轴线旋转样品处理装置的马达和相对于样品处理装置可操作地定位的光学模块，并配置成确定所选择的材料体积 存在于样品处理装置的检测室中。 该方法可以包括围绕旋转轴旋转样品处理装置，并且在旋转样品处理装置的同时确定所选择的材料体积是否存在于检测室中。 在一些实施例中，确定所选择的材料体积是否存在可以通过光学询问该检测室以获得该材料的光学特性。