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1.发明授权Methods for modifying cell motility using factor VIIa or inactivated factor VIIa 有权使用因子VIIa或灭活因子Ⅶa修饰细胞运动的方法公开(公告)号：US06461610B1
公开(公告)日：2002-10-08
申请号：US09372888
申请日：1999-08-12
申请人： Lars Kongsbak , Niels Bergenhem , Lars Christian Petersen , Ole Thastrup , Don Foster , Mirella Ezban , Agneta Siegbahn
发明人： Lars Kongsbak , Niels Bergenhem , Lars Christian Petersen , Ole Thastrup , Don Foster , Mirella Ezban , Agneta Siegbahn
IPC分类号： A61K3848
CPC分类号： G01N33/5041 , A61K38/4846 , G01N33/5008 , G01N33/5011 , G01N33/5035 , G01N33/5044 , G01N33/5064 , G01N33/86 , G01N2333/96447
摘要： The present invention provides methods for modifying the motility of tissue factor (TF)-expressing cells, which are carried out by contacting such cells with an effective amount of Factor VIIai Factor VIIa agonist.
摘要翻译： 本发明提供了通过使这些细胞与有效量的因子VIIai因子VIIa激动剂接触而进行的改变组织因子（TF）表达细胞运动性的方法。
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公开(公告)号：US20070232542A1
公开(公告)日：2007-10-04
申请号：US11755470
申请日：2007-05-30
申请人： Lars Kongsbak , Niels Bergenhem , Lars Petersen , Ole Thastrup , Don Foster , Mirella Ezban , Agneta Siegbahn
发明人： Lars Kongsbak , Niels Bergenhem , Lars Petersen , Ole Thastrup , Don Foster , Mirella Ezban , Agneta Siegbahn
IPC分类号： A61K38/36
CPC分类号： G01N33/5041 , A61K38/36 , A61K38/4846 , G01N33/5008 , G01N33/5011 , G01N33/5044 , G01N33/5064 , G01N33/86 , G01N2333/96447
摘要： A novel intracellular signalling activity of coagulation factor VII (FVII) in cells expressing tissue factor (TF) is described. The present invention relates to use of FVIIa or another TF agonist, or FVIIai or another TF antagonist for the preparation of a medicament for modulation of FVIIa-induced activation of the MAPK signalling pathway in a patient. Moreover the present invention relates to a method of treatment, and a method of detecting the activity of compounds, in particular drug candidates, that interact with the FVIIa mediated intracellular signalling pathway.
摘要翻译： 描述了表达组织因子（TF）的细胞中凝血因子VII（FVII）的新型细胞内信号传导活性。 本发明涉及FVIIa或另一种TF激动剂或FVIIa或另一种TF拮抗剂在制备用于调节FVIIa诱导的患者中MAPK信号通路的激活的药物中的用途。 此外，本发明涉及一种治疗方法和检测与FVIIa介导的细胞内信号传导途径相互作用的化合物，特别是候选药物的活性的方法。
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3.发明申请公开(公告)号：US20050245449A1
公开(公告)日：2005-11-03
申请号：US11175639
申请日：2005-07-06
申请人： Lars Kongsbak , Niels Bergenhem , Lars Petersen , Ole Thastrup , Don Foster , Mirella Ezban , Agneta Siegbahn
发明人： Lars Kongsbak , Niels Bergenhem , Lars Petersen , Ole Thastrup , Don Foster , Mirella Ezban , Agneta Siegbahn
CPC分类号： G01N33/5041 , A61K38/36 , A61K38/4846 , G01N33/5008 , G01N33/5011 , G01N33/5044 , G01N33/5064 , G01N33/86 , G01N2333/96447
摘要： A novel intracellular signalling activity of coagulation factor VII (FVII) in cells expressing tissue factor (TF) is described. The present invention relates to use of FVIIa or another TF agonist, or FVIIai or another TF antagonist for the preparation of a medicament for modulation of FVIIa-induced activation of the MAPK signalling pathway in a patient. Moreover the present invention relates to a method of treatment, and a method of detecting the activity of compounds, in particular drug candidates, that interact with the FVIIa mediated intracellular signalling pathway.
摘要翻译： 描述了表达组织因子（TF）的细胞中凝血因子VII（FVII）的新型细胞内信号传导活性。 本发明涉及FVIIa或另一种TF激动剂或FVIIa或另一种TF拮抗剂在制备用于调节FVIIa诱导的患者中MAPK信号通路的激活的药物中的用途。 此外，本发明涉及一种治疗方法和检测与FVIIa介导的细胞内信号传导途径相互作用的化合物，特别是候选药物的活性的方法。
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4.发明授权Identification of drug candidates modulating factor VII-mediated intracellular signaling 失效鉴定药物候选物调节因子VII介导的细胞内信号传导公开(公告)号：US06268163B1
公开(公告)日：2001-07-31
申请号：US09116748
申请日：1998-07-16
IPC分类号： C12Q148
CPC分类号： G01N33/5008 , A61K38/4846 , G01N33/5011 , G01N33/5035 , G01N33/5041 , G01N33/5044 , G01N33/5064 , G01N33/86 , G01N2333/96447
摘要： The present invention provides methods for identifying candidate drugs that modulate factor VIIa-mediated intracellular signaling by measuring the effects of such drugs on the activation of the mitogen-activated protein kinase (MAP kinase) signaling pathway.
摘要翻译： 本发明提供了通过测量这些药物对丝裂原活化蛋白激酶（MAP激酶）信号通路的激活的影响来鉴定调节因子VIIa介导的细胞内信号传导的候选药物的方法。
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公开(公告)号：US07314915B2
公开(公告)日：2008-01-01
申请号：US10947178
申请日：2004-09-23
IPC分类号： C07K14/00
CPC分类号： C07K14/43595 , C07K2319/00 , C12Q1/48 , Y10S530/808 , Y10S530/857
摘要： The present invention relates to novel variants of the fluorescent protein GFP having improved fluorescence properties.
摘要翻译： 本发明涉及具有改善的荧光性质的荧光蛋白GFP的新变体。
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6.发明授权Method of detecting biologically active substances by using green fluorescent protein 失效采用绿色荧光蛋白检测生物活性物质的方法
公开(公告)号：US5958713A
公开(公告)日：1999-09-28
申请号：US818604
申请日：1997-03-14
IPC分类号： C07K14/435 , C12N15/65 , G01N33/50 , G01N33/533 , G01N33/542 , C12Q1/00 , C12N5/10 , C12N15/12
CPC分类号： C07K14/43595 , C12N15/65 , G01N33/5005 , G01N33/533 , G01N33/542 , C07K2319/00 , G01N2333/43595 , G01N2333/9121
摘要： The present invention provides a method for detecting a biologically active substance that affects intracellular processes mediated through a protein kinase or second messenger, which comprises incubating a cell with a test substance and measuring any change caused by the test substance in the fluorescence of (i) green fluorescent protein (GFP) having a protein kinase recognition site or (ii) GFP that has been modified to contain a second messenger binding domain.
摘要翻译： 本发明提供了一种检测影响通过蛋白激酶或第二信使介导的细胞内过程的生物活性物质的方法，其包括将细胞与测试物质一起孵育并测量由测试物质引起的荧光（i） 具有蛋白激酶识别位点的绿色荧光蛋白（GFP）或（ii）经修饰以含有第二信使结合结构域的GFP。
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公开(公告)号：US06566083B1
公开(公告)日：2003-05-20
申请号：US09346946
申请日：1999-07-02
IPC分类号： G01N33573
CPC分类号： C07K14/43595 , C07K2319/00 , C12N15/65 , G01N33/5005 , G01N33/533 , G01N33/542 , G01N2333/43595 , G01N2333/9121
摘要： The present invention relates to a method of detecting biologically active substances affecting intracellular processes, an isolated DNA, a vector and a cell for use in the method. More specifically, the present invention provides a method for detecting a biologically active substance that affects intracellular processes mediated through a protein kinase or a second messenger, which comprises incubating a cell with a test substance and measuring any change caused by the test substance in the flourescence of (i) a wild-type or modified green flourescent protein (GFP) having a protein kinase recognition site, (ii) a modified GFP containing a second messenger binding domain, or (iii) a hybrid polypeptide having a wild-type or modified GFP and an attached protein kinase recognition site or a second messenger binding domain.
摘要翻译： 本发明涉及检测影响细胞内过程的生物活性物质的方法，分离的DNA，载体和用于该方法的细胞。 更具体地，本发明提供了一种检测影响通过蛋白激酶或第二信使介导的细胞内过程的生物活性物质的方法，其包括将细胞与测试物质一起孵育并测量由荧光检测物质引起的任何变化 （i）具有蛋白激酶识别位点的野生型或修饰型绿色荧光蛋白（GFP），（ii）含有第二信使结合结构域的修饰型GFP，或（iii）具有野生型或修饰型的杂合多肽 GFP和附着的蛋白激酶识别位点或第二信使结合结构域。
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公开(公告)号：US06818443B2
公开(公告)日：2004-11-16
申请号：US09872364
申请日：2001-06-01
IPC分类号： C12N1563
CPC分类号： C07K14/43595 , C07K2319/00 , C12Q1/48 , Y10S530/808 , Y10S530/857
摘要： The present invention relates to novel variants of the fluorescent protein GFP having improved fluorescence properties.
摘要翻译： 本发明涉及具有改善的荧光性质的荧光蛋白GFP的新变体。
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公开(公告)号：US06172188B2
公开(公告)日：2001-01-09
申请号：US08819612
申请日：1997-03-17
IPC分类号： C07K100
CPC分类号： C07K14/43595 , C07K2319/00 , C12Q1/48 , Y10S530/808 , Y10S530/857
摘要： The present invention relates to novel variants of the fluorescent protein GFP having improved fluorescence properties.
摘要翻译： 本发明涉及具有改善的荧光性质的荧光蛋白GFP的新变体。
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公开(公告)号：US20130123140A1
公开(公告)日：2013-05-16
申请号：US13441091
申请日：2012-04-06
申请人： Ole Thastrup , Morten Meldal , Grith Hagel , Jens Chr. Norrild , Morten Hentzer
发明人： Ole Thastrup , Morten Meldal , Grith Hagel , Jens Chr. Norrild , Morten Hentzer
IPC分类号： C12Q1/02
CPC分类号： C12Q1/025 , C07K1/047 , C07K5/1008 , C07K7/06 , C07K7/08 , C40B30/06 , G01N33/5023 , G01N33/54313 , G01N33/6845 , G01N2035/00158 , G01N2500/10
摘要： The present invention relates to methods for identifying compounds capable of modulating a cellular response. The methods involve attaching living cells to solid supports comprising a library of test compounds. The test compounds are linked to the solid support via cleavable linkers and may thus be released from the solid supports. Solid supports comprising cells, wherein the cellular response of interest has been modulated are selected and the test compound of the solid support can then be identified. The cellular response may for example be changes in complex formation between proteins.
摘要翻译： 本发明涉及鉴定能调节细胞反应的化合物的方法。 所述方法包括将活细胞连接到包含测试化合物文库的固体支持物上。 测试化合物通过可切割接头连接到固体支持物上，因此可以从固体支持物释放。 选择包含细胞的固体支持物，其中调节感兴趣的细胞应答，然后可以鉴定固体支持物的测试化合物。 细胞反应可能例如是蛋白质之间复合物形成的变化。
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