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公开(公告)号：US08367325B2
公开(公告)日：2013-02-05
申请号：US11701428
申请日：2007-02-02
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6806 , C12Q1/6851 , C12Q2561/113 , C12Q2531/107 , C12Q2527/107
摘要： A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.
摘要翻译： 使用浓度调节的熔点至少等于并且优选超过过量底漆的极限底物的低浓度限制性引物的非对称聚合链反应（PCR）扩增方法，后者又不超过25℃ 低于扩增子的解链温度。 使用这种扩增和标记的杂交探针的测定法，包括在引物延伸或低温探针之后包括检测步骤的测定法或两者。 用于进行此类测定的试剂盒和用于进行前述扩增和测定的引物或引物 - 探针组。
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2.发明申请公开(公告)号：US20090226973A1
公开(公告)日：2009-09-10
申请号：US12382670
申请日：2009-03-20
申请人： Lawrence J. Wangh , John Rice , J. Aquiles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
发明人： Lawrence J. Wangh , John Rice , J. Aquiles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
CPC分类号： C12Q1/6848 , C12Q1/686 , C12Q2527/125 , C12Q2525/301 , C12Q2525/186 , C12Q2531/107 , C12Q2527/137
摘要： An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.
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3.发明授权Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification 有权用于提高再现性的试剂和方法，并减少PCR扩增中的错误引物公开(公告)号：US07517977B2
公开(公告)日：2009-04-14
申请号：US11252506
申请日：2005-10-17
申请人： Lawrence J. Wangh , John Rice , J. Aquiles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
发明人： Lawrence J. Wangh , John Rice , J. Aquiles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
CPC分类号： C12Q1/6848 , C12Q1/686 , C12Q2527/125 , C12Q2525/301 , C12Q2525/186 , C12Q2531/107 , C12Q2527/137
摘要： An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.
摘要翻译： 用于防止聚合酶链反应（PCR）扩增和测定中的错配的添加剂，其包含具有长度超过六个核苷酸的茎双螺旋的发夹寡核苷酸和稳定的茎末端。 当添加到初始扩增反应混合物中时，添加剂改进PCR扩增，包括LATE-PCR扩增。 它可以包括在寡核苷酸组和用于PCR扩增和测定的试剂盒中。
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公开(公告)号：US20130210656A1
公开(公告)日：2013-08-15
申请号：US13734684
申请日：2013-01-04
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6806 , C12Q1/6851 , C12Q2561/113 , C12Q2531/107 , C12Q2527/107
摘要： A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.
摘要翻译： 使用浓度调节的熔点至少等于并且优选超过过量底漆的极限底物的低浓度限制性引物的非对称聚合链反应（PCR）扩增方法，后者又不超过25℃ 低于扩增子的解链温度。 使用这种扩增和标记的杂交探针的测定法，包括在引物延伸或低温探针之后包括检测步骤的测定法或两者。 用于进行此类测定的试剂盒和用于进行前述扩增和测定的引物或引物 - 探针组。
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公开(公告)号：US20120040352A1
公开(公告)日：2012-02-16
申请号：US12292038
申请日：2008-11-10
申请人： Lawrence J. Wangh , John Rice , J. Aquiles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
发明人： Lawrence J. Wangh , John Rice , J. Aquiles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q1/6851 , C12Q1/6876 , C12Q2600/156 , C12Q2600/16 , C12Q2545/114 , C12Q2531/107 , C12Q2527/107 , C12Q2561/113
摘要： Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.
摘要翻译： 使用荧光DNA染料和间接可激发标记的引物和探针进行PCR扩增期间或之后的均质检测，优选LATE-PCR，提高了重现性和定量。 在非对称PCR扩增期间或之后的低温均相检测，优选LATE-PCR，利用荧光DNA染料和间接可激发标记的错配耐受性探针允许分析复杂的靶标。 LATE-PCR扩增后的测序样品制备方法降低了复杂性并允许“单管”处理。
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公开(公告)号：US07198897B2
公开(公告)日：2007-04-03
申请号：US10320893
申请日：2002-12-17
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6806 , C12Q1/6851 , C12Q2561/113 , C12Q2531/107 , C12Q2527/107
摘要： A non-symmetric polymerase chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.
摘要翻译： 使用浓度调节的熔点至少等于并且优选超过过量引物的极限引物的低浓度限制性引物的非对称聚合酶链式反应（PCR）扩增方法，后者又不超过25℃ 低于扩增子的解链温度。 使用这种扩增和标记的杂交探针的测定法，包括在引物延伸或低温探针之后包括检测步骤的测定法或两者。 用于进行此类测定的试剂盒和用于进行前述扩增和测定的引物或引物 - 探针组。
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公开(公告)号：US07632642B2
公开(公告)日：2009-12-15
申请号：US11252433
申请日：2005-10-17
申请人： Lawrence J. Wangh , John Rice , J. Aquiles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
发明人： Lawrence J. Wangh , John Rice , J. Aquiles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q1/6851 , C12Q1/6876 , C12Q2600/156 , C12Q2600/16 , C12Q2545/114 , C12Q2531/107 , C12Q2527/107 , C12Q2561/113
摘要： Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.
摘要翻译： 使用荧光DNA染料和间接可激发标记的引物和探针进行PCR扩增期间或之后的均质检测，优选LATE-PCR，提高了重现性和定量。 在非对称PCR扩增期间或之后的低温均相检测，优选LATE-PCR，利用荧光DNA染料和间接可激发标记的错配耐受性探针允许分析复杂的靶标。 LATE-PCR扩增后的测序样品制备方法降低了复杂性并允许“单管”处理。
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公开(公告)号：US20080280292A1
公开(公告)日：2008-11-13
申请号：US11701428
申请日：2007-02-02
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6806 , C12Q1/6851 , C12Q2561/113 , C12Q2531/107 , C12Q2527/107
摘要： A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.
摘要翻译： 使用浓度调节的熔点至少等于并且优选超过过量底漆的极限底物的低浓度限制性引物的非对称聚合链反应（PCR）扩增方法，后者又不超过25℃ 低于扩增子的解链温度。 使用这种扩增和标记的杂交探针的测定法，包括在引物延伸或低温探针之后包括检测步骤的测定法或两者。 用于进行此类测定的试剂盒和用于进行前述扩增和测定的引物或引物 - 探针组。
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公开(公告)号：US20130095479A1
公开(公告)日：2013-04-18
申请号：US13564528
申请日：2012-08-01
申请人： Lawrence J. Wangh , John Rice , J. Aquilles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
发明人： Lawrence J. Wangh , John Rice , J. Aquilles Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q1/6851 , C12Q1/6876 , C12Q2600/156 , C12Q2600/16 , C12Q2545/114 , C12Q2531/107 , C12Q2527/107 , C12Q2561/113
摘要： Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.
摘要翻译： 使用荧光DNA染料和间接可激发标记的引物和探针进行PCR扩增期间或之后的均质检测，优选LATE-PCR，提高了重现性和定量。 在非对称PCR扩增期间或之后的低温均相检测，优选LATE-PCR，利用荧光DNA染料和间接可激发标记的错配耐受性探针允许分析复杂的靶标。 LATE-PCR扩增后的测序样品制备方法降低了复杂性并允许“单管”处理。
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10.发明申请公开(公告)号：US20060177842A1
公开(公告)日：2006-08-10
申请号：US11252506
申请日：2005-10-17
申请人： Lawrence Wangh , John Rice , J. Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
发明人： Lawrence Wangh , John Rice , J. Sanchez , Kenneth Pierce , Jesse Salk , Arthur Reis , Cristina Hartshorn
CPC分类号： C12Q1/6848 , C12Q1/686 , C12Q2527/125 , C12Q2525/301 , C12Q2525/186 , C12Q2531/107 , C12Q2527/137
摘要： An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.
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