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公开(公告)号:US06428993B1
公开(公告)日:2002-08-06
申请号:US08881742
申请日:1997-06-24
申请人: Lisbeth Bech , Grethe Rasmussen , Torben Halkier , Mariko Okada , Lene Nonboe Andersen , Markus Sakari Kauppinen , Thomas Sandal
发明人: Lisbeth Bech , Grethe Rasmussen , Torben Halkier , Mariko Okada , Lene Nonboe Andersen , Markus Sakari Kauppinen , Thomas Sandal
IPC分类号: C12N910
CPC分类号: C12Y203/02013 , A21D8/042 , C12N9/1044 , C14C11/00 , Y10S435/911
摘要: The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.
摘要翻译: 本发明涉及从属于卵菌纲的较低真菌获得的转谷氨酰胺酶和转谷氨酰胺酶制剂。 通过生长这些细胞形成的细菌,特别是菌株腐霉菌,不锈钢腐霉,腐霉腐霉(P. periplocum),腐皮腐,腐霉,腐霉,腐皮疫霉,致病疫霉(Phytophthora palmivora),可以实现前所未有的高水平表达 ,疫霉疫霉(Phytophthora porri),致病疫霉(Phytophthora infestans),致病疫霉(Phytoph-thora megasperma),肉桂疫霉(Phytophthora cinnamomi)和疫霉疫霉(Phytophthora cryptogea)。 克隆和表达重组转谷氨酰胺酶,酶和酶制剂可用于交联蛋白质,例如, 在面粉,烘焙食品,肉制品,鱼制品,化妆品,奶酪,乳制品,凝胶食品和皮革整理中,或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。
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公开(公告)号:US07094586B2
公开(公告)日:2006-08-22
申请号:US10164765
申请日:2002-06-07
申请人: Lisbeth Bech , Grethe Rasmussen , Torben Halkier , Mariko Okada , Lene Nonboe Andersen , Markus Sakari Kauppinen , Thomas Sandal
发明人: Lisbeth Bech , Grethe Rasmussen , Torben Halkier , Mariko Okada , Lene Nonboe Andersen , Markus Sakari Kauppinen , Thomas Sandal
CPC分类号: C12Y203/02013 , A21D8/042 , C12N9/1044 , C14C11/00 , Y10S435/911
摘要: The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes and unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytophthora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea; and a recombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.
摘要翻译: 本发明涉及从属于卵菌纲的低级真菌得到的转谷氨酰胺酶和转谷氨酰胺酶制剂,并且具有前所未有的高水平表达是通过生长这些形成细胞的生物体,特别是菌株腐霉菌,非腐霉菌,不锈菌腐霉,腐霉腐霉(或 P.腐生菌),腐皮腐,腐霉,腐霉,腐皮疫霉,疫霉疫霉,致病疫霉,致热疫霉,肉,疫霉和疫霉疫霉;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;; 并且克隆和表达重组转谷氨酰胺酶,所述酶和酶制剂可用于交联蛋白质,例如, 在面粉,烘焙食品,肉制品,鱼制品,化妆品,奶酪,乳制品,凝胶食品和皮革整理中,或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。
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公开(公告)号:US07235253B2
公开(公告)日:2007-06-26
申请号:US09994773
申请日:2001-11-28
申请人: Yoshinobu Hanyu , Mariko Okada , Chihiro Shindo , Satoshi Nishimuro , Tetsuo Yokoyama , Masato Horie
发明人: Yoshinobu Hanyu , Mariko Okada , Chihiro Shindo , Satoshi Nishimuro , Tetsuo Yokoyama , Masato Horie
CPC分类号: A61K9/0075 , A61K9/1617 , A61K9/1623 , A61K9/1635 , A61K9/1652 , A61K38/27
摘要: A method is disclosed for stabilizing a physiologically active peptide in a process of preparing a powder containing the physiologically active peptide by drying an aqueous liquid containing the physiologically active peptide, wherein the method comprises adding to the aqueous liquid at least one compound selected from the group consisting of a nonionic surfactant, a water-soluble, nonionic, organic binder, hydrogenated lecithin, and mannitol.
摘要翻译: 公开了一种在制备含有生理活性肽的粉末的方法中通过干燥含有生理活性肽的水性液体来稳定生理活性肽的方法,其中该方法包括向水性液体中加入至少一种选自 由非离子表面活性剂,水溶性,非离子,有机粘合剂,氢化卵磷脂和甘露醇组成。
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