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11 条记录 (耗时 0.03 秒)

    Transglutaminase from oomycetes
    1.
    发明授权
    Transglutaminase from oomycetes 失效
    来自卵菌的转谷氨酰胺酶

    公开(公告)号：US06428993B1

    公开(公告)日：2002-08-06

    申请号：US08881742

    申请日：1997-06-24

    申请人： Lisbeth Bech ,  Grethe Rasmussen ,  Torben Halkier ,  Mariko Okada ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Thomas Sandal

    发明人： Lisbeth Bech ,  Grethe Rasmussen ,  Torben Halkier ,  Mariko Okada ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Thomas Sandal

    IPC分类号： C12N910

    CPC分类号： C12Y203/02013 ,  A21D8/042 ,  C12N9/1044 ,  C14C11/00 ,  Y10S435/911

    摘要： The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.

    摘要翻译： 本发明涉及从属于卵菌纲的较低真菌获得的转谷氨酰胺酶和转谷氨酰胺酶制剂。 通过生长这些细胞形成的细菌，特别是菌株腐霉菌，不锈钢腐霉，腐霉腐霉（P. periplocum），腐皮腐，腐霉，腐霉，腐皮疫霉，致病疫霉（Phytophthora palmivora），可以实现前所未有的高水平表达 ，疫霉疫霉（Phytophthora porri），致病疫霉（Phytophthora infestans），致病疫霉（Phytoph-thora megasperma），肉桂疫霉（Phytophthora cinnamomi）和疫霉疫霉（Phytophthora cryptogea）。 克隆和表达重组转谷氨酰胺酶，酶和酶制剂可用于交联蛋白质，例如， 在面粉，烘焙食品，肉制品，鱼制品，化妆品，奶酪，乳制品，凝胶食品和皮革整理中，或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。

    Transglutaminase from oomycetes
    2.
    发明授权
    Transglutaminase from oomycetes 失效
    来自卵菌的转谷氨酰胺酶

    公开(公告)号：US07094586B2

    公开(公告)日：2006-08-22

    申请号：US10164765

    申请日：2002-06-07

    申请人： Lisbeth Bech ,  Grethe Rasmussen ,  Torben Halkier ,  Mariko Okada ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Thomas Sandal

    发明人： Lisbeth Bech ,  Grethe Rasmussen ,  Torben Halkier ,  Mariko Okada ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Thomas Sandal

    IPC分类号： C12N9/10 ,  C12N15/54

    CPC分类号： C12Y203/02013 ,  A21D8/042 ,  C12N9/1044 ,  C14C11/00 ,  Y10S435/911

    摘要： The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes and unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytophthora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea; and a recombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.

    摘要翻译： 本发明涉及从属于卵菌纲的低级真菌得到的转谷氨酰胺酶和转谷氨酰胺酶制剂，并且具有前所未有的高水平表达是通过生长这些形成细胞的生物体，特别是菌株腐霉菌，非腐霉菌，不锈菌腐霉，腐霉腐霉（或 P.腐生菌），腐皮腐，腐霉，腐霉，腐皮疫霉，疫霉疫霉，致病疫霉，致热疫霉，肉，疫霉和疫霉疫霉;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;; 并且克隆和表达重组转谷氨酰胺酶，所述酶和酶制剂可用于交联蛋白质，例如， 在面粉，烘焙食品，肉制品，鱼制品，化妆品，奶酪，乳制品，凝胶食品和皮革整理中，或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。

    Method of providing a hybrid polypeptide exhibiting an activity of interest
    5.
    发明授权
    Method of providing a hybrid polypeptide exhibiting an activity of interest 失效
    提供表现出感兴趣活性的杂合多肽的方法

    公开(公告)号：US06270968B1

    公开(公告)日：2001-08-07

    申请号：US09189060

    申请日：1998-11-05

    申请人： Henrik Dalbøge ,  Thomas Sandal ,  Markus Sakari Kauppinen ,  Børge Diderichsen

    发明人： Henrik Dalbøge ,  Thomas Sandal ,  Markus Sakari Kauppinen ,  Børge Diderichsen

    IPC分类号： C12Q168

    CPC分类号： C12N15/1044 ,  C12N9/2437 ,  C12N9/248 ,  C12Q1/6811 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01032

    摘要： A method for providing a hybrid polypeptide having an activity of interest, by i) performing PCR amplification using an uncharacterized DNA sample and oligonucleotide primers with homology to one or more known genes encoding a polypeptide exhibiting said activity of interest, to obtain one or more PCR products, ii) linking the obtained PCR products to a 5′ structural gene sequence and a 3′ structural gene sequence, wherein the 5′ and 3′ structural gene sequences are derived from one or more genes encoding a polypeptide exhibiting said activity of interest, to form hybrid DNA sequences, iii) expressing the resulting hybrid DNA sequences, and iv) screening the hybrid polypeptides to identify a sequence encoding a polypeptide exhibiting said activity of interest or a related activity.

    摘要翻译： 一种用于提供具有感兴趣活性的杂交多肽的方法，其通过以下步骤：i）使用未表征的DNA样品进行PCR扩增和与编码显示所述目的活性的多肽的一种或多种已知基因同源的寡核苷酸引物，以获得一种或多种PCR 产品，ii）将获得的PCR产物连接到5'结构基因序列和3'结构基因序列，其中5'和3'结构基因序列衍生自编码显示所述目标活性的多肽的一个或多个基因， 以形成杂交DNA序列，iii）表达所得的杂交DNA序列，和iv）筛选杂合多肽以鉴定编码显示所述目的活性或相关活性的多肽的序列。

    Enzyme with lipolytic activity
    7.
    发明授权
    Enzyme with lipolytic activity 失效
    具有脂肪分解活性的酶

    公开(公告)号：US5827719A

    公开(公告)日：1998-10-27

    申请号：US817997

    申请日：1997-04-25

    申请人： Thomas Sandal ,  Sakari Kauppinen ,  Lene Venke Kofod

    发明人： Thomas Sandal ,  Sakari Kauppinen ,  Lene Venke Kofod

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/15 ,  C12N9/20 ,  C12N15/00 ,  C12R1/645 ,  C12R1/865 ,  C12N15/55 ,  C12N15/63 ,  C12P21/02

    CPC分类号： C12N9/20

    摘要： The invention relates, inter alia, to a DNA construct comprising a DNA sequence which encodes an enzyme exhibiting lipolytic activity and which comprises: a) the DNA sequence shown in SEQ ID No. 1, or b) an analogue of the DNA sequence shown in SEQ ID No. 1 which i) is homologous with the DNA sequence shown in SEQ ID No. 1, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 1, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 1, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified lipolytic enzyme which is encoded by the DNA sequence shown in SEQ ID No. 1 and/or which is derived from Humicola insolens DSM 1800. Also disclosed, inter alia, are: lipolytic enzymes encoded by such DNA constructs; and enzyme preparations, detergent additives and detergent compositions comprising such lipolytic enzymes.

    摘要翻译： PCT No.PCT / DK95 / 00427 Sec。 371日期1997年04月25日 102（e）日期1997年4月25日PCT提交1995年10月26日PCT公布。 公开号WO96 / 13580 日期1996年5月9日本发明尤其涉及包含编码表现出脂肪分解活性的酶的DNA序列的DNA构建体，其包含：a）SEQ ID No.1所示的DNA序列，或b） 序列号1所示的DNA序列，其i）与SEQ ID No.1所示的DNA序列同源，和/或ii）与与SEQ ID No.1所示的DNA序列相同的寡核苷酸探针杂交， /或iii）编码与由包含SEQ ID No.1所示的DNA序列的DNA序列编码的多肽同源的多肽，和/或iv）编码与针对纯化的脂肪分解产生的抗体具有免疫反应性的多肽 由SEQ ID No.1所示的DNA序列和/或源自Humicola insolens DSM 1800的DNA序列编码的酶。特别地，还公开了由这些DNA构建体编码的脂肪分解酶。 和酶制剂，洗涤剂添加剂和包含这种脂肪分解酶的洗涤剂组合物。

    MODIFIED PROTEINS AND PEPTIDES
    8.
    发明申请
    MODIFIED PROTEINS AND PEPTIDES 审中-公开
    改性蛋白和肽

    公开(公告)号：US20140227259A1

    公开(公告)日：2014-08-14

    申请号：US14239196

    申请日：2012-08-13

    申请人： Claire Ashman ,  Mary Birchler ,  Rudolf M.T. De Wildt ,  Claire Holland ,  Alan Peter Lewis ,  Peter Morley ,  Thomas Sandal ,  Michael Steward

    发明人： Claire Ashman ,  Mary Birchler ,  Rudolf M.T. De Wildt ,  Claire Holland ,  Alan Peter Lewis ,  Peter Morley ,  Thomas Sandal ,  Michael Steward

    IPC分类号： C07K16/42

    摘要： The present disclosure relates to modified proteins and peptides that have reduced ability to bind to pre-existing antibodies. Such modified protein/peptide molecules can comprise C-terminal additions, extensions or tags and/or certain amino acid substitutions. Such modified molecules (e.g., fusions and conjugates) comprise proteins, peptides, antigen binding molecules, antibodies or antibody fragments such as single variable domains. The disclosure further relates to uses and formulations of compositions comprising such modified C-terminally extended and/or amino acid substituted molecules and also to methods of production and expression of these.

    摘要翻译： 本公开涉及具有降低与先前存在的抗体结合的能力的修饰的蛋白质和肽。 这种修饰的蛋白质/肽分子可以包含C-末端添加，延伸或标签和/或某些氨基酸取代。 这样的修饰分子（例如，融合物和缀合物）包含蛋白质，肽，抗原结合分子，抗体或抗体片段，例如单可变结构域。 本公开还涉及包含这种修饰的C-末端延伸和/或氨基酸取代的分子的组合物的用途和制剂，以及这些的生产和表达方法。