公开(公告)号：US06448013B1

公开(公告)日：2002-09-10

申请号：US09721801

申请日：2000-11-22

申请人： Lyle Arnold ,  Tod Bedilion ,  Erik Bieldanes ,  Thomas P. Theriault ,  Warren Lei

发明人： Lyle Arnold ,  Tod Bedilion ,  Erik Bieldanes ,  Thomas P. Theriault ,  Warren Lei

IPC分类号： C12Q168

CPC分类号： C12Q1/6837 ,  C12Q1/6851 ,  Y10T436/143333 ,  C12Q2545/114 ,  C12Q2531/113 ,  C12Q2565/501

摘要： Methods are provide for quantitatively determining the amount of polynucleotides in a sample. In one method, the distinct polynucleotide targets and standard polynucleotide targets are contacted with detectable probes and independently detectable single or double stranded complements to the standard targets under hybridization conditions. The hybridization pattern from the probe is compared to the hybridization pattern from the standard sequences to obtain quantitative information about the genetic profile of the labeled nucleic acid sample. Also provided are kits for the use of the methods.

摘要翻译： 提供了定量测定样品中多核苷酸的量的方法。 在一种方法中，在杂交条件下，将不同的多核苷酸靶标和标准多核苷酸靶标与可检测的探针和标准靶的独立可检测的单链或双链互补物接触。 将来自探针的杂交模式与来自标准序列的杂交模式进行比较，以获得关于标记的核酸样品的遗传图谱的定量信息。 还提供了使用方法的工具包。