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    Duplex probes for hybridization reactions
    1.
    发明授权
    Duplex probes for hybridization reactions 失效
    用于杂交反应的双相探针

    公开(公告)号：US06448013B1

    公开(公告)日：2002-09-10

    申请号：US09721801

    申请日：2000-11-22

    申请人： Lyle Arnold ,  Tod Bedilion ,  Erik Bieldanes ,  Thomas P. Theriault ,  Warren Lei

    发明人： Lyle Arnold ,  Tod Bedilion ,  Erik Bieldanes ,  Thomas P. Theriault ,  Warren Lei

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6837 ,  C12Q1/6851 ,  Y10T436/143333 ,  C12Q2545/114 ,  C12Q2531/113 ,  C12Q2565/501

    摘要： Methods are provide for quantitatively determining the amount of polynucleotides in a sample. In one method, the distinct polynucleotide targets and standard polynucleotide targets are contacted with detectable probes and independently detectable single or double stranded complements to the standard targets under hybridization conditions. The hybridization pattern from the probe is compared to the hybridization pattern from the standard sequences to obtain quantitative information about the genetic profile of the labeled nucleic acid sample. Also provided are kits for the use of the methods.

    摘要翻译： 提供了定量测定样品中多核苷酸的量的方法。 在一种方法中，在杂交条件下，将不同的多核苷酸靶标和标准多核苷酸靶标与可检测的探针和标准靶的独立可检测的单链或双链互补物接触。 将来自探针的杂交模式与来自标准序列的杂交模式进行比较，以获得关于标记的核酸样品的遗传图谱的定量信息。 还提供了使用方法的工具包。

    Normalization control for hybridization reactions
    2.
    发明授权
    Normalization control for hybridization reactions 失效
    杂交反应的归一化控制

    公开(公告)号：US06423535B1

    公开(公告)日：2002-07-23

    申请号：US09449059

    申请日：1999-11-24

    申请人： Lyle Arnold ,  Tod Bedilion ,  Erik Bjeldanes ,  Thomas P. Theriault ,  Warren Lei

    发明人： Lyle Arnold ,  Tod Bedilion ,  Erik Bjeldanes ,  Thomas P. Theriault ,  Warren Lei

    IPC分类号： C12M134

    CPC分类号： C12Q1/6837 ,  C12Q1/6851 ,  Y10T436/143333 ,  C12Q2545/114 ,  C12Q2531/113 ,  C12Q2565/501

    摘要： Methods are provide for quantitatively determining the amount of polynucleotides in a sample. In one method, the distinct polynucleotide targets and standard polynucleotide targets are contacted with detectable probes and independently detectable single or double stranded complements to the standard targets under hybridization conditions. The hybridization pattern from the probe is compared to the hybridization pattern from the standard sequences to obtain quantitative information about the genetic profile of the labeled nucleic acid sample. Also provided are kits for the use of the methods.

    摘要翻译： 提供了定量测定样品中多核苷酸的量的方法。 在一种方法中，在杂交条件下，将不同的多核苷酸靶标和标准多核苷酸靶标与可检测的探针和标准靶标的独立可检测的单链或双链互补物接触。 将来自探针的杂交模式与来自标准序列的杂交模式进行比较，以获得关于标记的核酸样品的遗传图谱的定量信息。 还提供了使用方法的工具包。

    SNP detection
    5.
    发明授权
    SNP detection 失效
    SNP检测

    公开(公告)号：US06410231B1

    公开(公告)日：2002-06-25

    申请号：US09259898

    申请日：1999-02-26

    申请人： Lyle Arnold ,  Thomas Theriault ,  Tod Bedilion

    发明人： Lyle Arnold ,  Thomas Theriault ,  Tod Bedilion

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6827 ,  C12Q1/6837

    摘要： The invention provides methods, compositions and systems for detecting multiple single nucleotide polymorphisms (SNPs) in a population of target polynucleotides in parallel in a sandwich assay employing SNP probes, capture polynucleotides and, optionally, auxiliary polynucleotides. The relative affinities of the SNP probes for the corresponding SNP regions can be increased with reagents which normalize the melting temperatures of the probes and/or by positionally facilitating interactions between the SNP probe, the SNP region, the capture polynucleotide and/or the auxiliary polynucleotides, such as through a minor groove binder. The probes may comprise a degenerate set of all possible same-sized polynucleotides and the capture polynucleotides are generally immobilized and arrayed at corresponding discrete elements in high density.

    摘要翻译： 本发明提供了在使用SNP探针，捕获多核苷酸和任选的辅助多核苷酸的夹心测定中并行检测多核苷酸多核苷酸群体中的多个单核苷酸多态性（SNP）的方法，组合物和系统。 SNP探针对相应SNP区域的相对亲和力可以通过使探针的解链温度正常化的试剂和/或通过位置促进SNP探针，SNP区域，捕获多核苷酸和/或辅助多核苷酸之间的相互作用的试剂来增加 ，例如通过小沟槽粘合剂。 探针可以包含所有可能的相同大小的多核苷酸的简并集，并且捕获多核苷酸通常被固定并以高密度排列在相应的离散元件上。

    Oligonucleotides comprising a molecular switch
    6.
    发明申请
    Oligonucleotides comprising a molecular switch 有权
    包含分子开关的寡核苷酸

    公开(公告)号：US20050042638A1

    公开(公告)日：2005-02-24

    申请号：US10837530

    申请日：2004-04-30

    申请人： Lyle Arnold ,  Bob Brown

    发明人： Lyle Arnold ,  Bob Brown

    IPC分类号： A61B20060101 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/6834 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2525/197 ,  C12Q2525/161

    摘要： This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.

    摘要翻译： 本发明涉及包含分子开关的寡核苷酸，分子开关可以存在于“开放”或“闭合”位置。 探针的分子开关部分对与分子开关互补的序列的身份特别敏感。 含有分子开关的寡核苷酸适用于各种杂交过程。 由于寡核苷酸的开关结构域的灵敏度，含有分子开关的探针在识别单点错配中特别有用。 更具体地，寡核苷酸的一部分但不是全部从失配的靶变得不结合。 本发明还涉及将所述寡核苷酸用于研究试剂和临床诊断的方法。 示例性寡核苷酸包含第一可杂交结构域，第二桥连嵌段结构域和第三结合结构域。

    Capture Probes Immobilizable Via L-Nucleotide Tail
    7.
    发明申请
    Capture Probes Immobilizable Via L-Nucleotide Tail 有权
    捕获探针可通过L-核苷酸尾部固定

    公开(公告)号：US20130260368A1

    公开(公告)日：2013-10-03

    申请号：US13823551

    申请日：2011-09-16

    申请人： Reinhold Pollner ,  Mehrdad Majlessi ,  Susan Yamagata ,  Michael M. Becker ,  Mark Reynolds ,  Lyle Arnold

    发明人： Reinhold Pollner ,  Mehrdad Majlessi ,  Susan Yamagata ,  Michael M. Becker ,  Mark Reynolds ,  Lyle Arnold

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/707 ,  C12Q1/6837 ,  C12Q1/703 ,  Y02A50/53 ,  Y02A50/54 ,  C12Q2525/161

    摘要： The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.

    摘要翻译： 本发明提供了通过可以在固定化探针中结合互补L-核酸的L-核酸尾部固定的嵌合捕获探针。 捕获探针可用于从样品中捕获靶核酸。 捕获探针尾部的L-核酸与固定化探针中的互补L-核酸结合，具有与否则相当于D-核酸的亲和力。 然而，捕获探针尾和固定化探针的L-核酸不与存在于含有靶核酸的样品中的D-核酸形成稳定的双链体。 将样品中的核酸直接与捕获探针的固定化探针或尾部的结合降低或消除，增加了测定的灵敏度和/或特异性。

    Single primer PCR amplification of RNA
    8.
    发明授权
    Single primer PCR amplification of RNA 失效
    RNA的单引物PCR扩增

    公开(公告)号：US06379932B1

    公开(公告)日：2002-04-30

    申请号：US09617578

    申请日：2000-07-17

    申请人： Lyle Arnold ,  Erik Bjeldanes ,  Steve Daniel

    发明人： Lyle Arnold ,  Erik Bjeldanes ,  Steve Daniel

    IPC分类号： C12P1934

    CPC分类号： C12Q1/6844 ,  C12N15/1096 ,  Y10T436/143333 ,  C12Q2521/107 ,  C12Q2525/173 ,  C12Q2521/319 ,  C12Q2525/179

    摘要： The invention provides methods and compositions for amplifying RNA sequences by (a) hybridizing to a target RNA a first primer comprising a 3′ target RNA hybridizing sequence and a first 5′ defined amplifyable sequence; (b) extending the first primer with a reverse transcriptase to form a first cDNA strand; (c) hybridizing to the first cDNA strand a second primer comprising a 3′ random cDNA hybridizing sequence and a second 5′ defined amplifyable sequence; (d) extending the second primer with a DNA polymerase to form a second cDNA strand; and (e) amplifying the second cDNA strand with a third primer comprising the first 5′ defined amplifyable sequence.

    摘要翻译： 本发明提供了通过以下步骤扩增RNA序列的方法和组合物：（a）与靶RNA杂交包含3'靶RNA杂交序列和第一个5'定义的可扩增序列的第一引物; （b）用逆转录酶延伸第一引物以形成第一个cDNA链; （c）与第一cDNA链杂交包含3'随机cDNA杂交序列和第二个5'定义的可扩增序列的第二引物; （d）用DNA聚合酶延伸第二引物以形成第二个cDNA链; 和（e）用包含前5'定义的可扩增序列的第三引物扩增第二cDNA链。

    Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe
    10.
    发明申请
    Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe 审中-公开
    通过使用核酸杂交开关探针检测分析物的组合物和方法

    公开(公告)号：US20060194240A1

    公开(公告)日：2006-08-31

    申请号：US11363848

    申请日：2006-02-28

    申请人： Lyle Arnold ,  Lizhong Dai ,  Steven Brentano ,  James Russell

    发明人： Lyle Arnold ,  Lizhong Dai ,  Steven Brentano ,  James Russell

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q2525/197 ,  C12Q2525/161 ,  C12Q2565/107 ,  C12Q2563/131 ,  C12Q2525/301

    摘要： Compositions are described for detecting binding of an analyte to a binding partner attached to a nucleic acid hybridization switch probe that includes first and second arm sequences and a support sequence that is at least partially complementary to both arm sequences, allowing the probe under hybridization conditions to form a first conformation in the absence of the analyte and to form a second conformation in the presence of the analyte, and a label associated with the probe that produces a signal that indicates the conformation of the probe. Methods are described for detecting an analyte that forms a specific binding pair with the binding partner attached to the hybridization switch probe, thereby changing the probe from a first to a second conformation that results in a detectable signal that indicates the presence of the analyte in the sample.

    摘要翻译： 描述了用于检测分析物与连接到核酸杂交开关探针的结合配偶体的结合的组合物，其包含第一和第二臂序列以及与两个臂序列至少部分互补的支持序列，允许探针在杂交条件下 在不存在分析物的情况下形成第一构象，并在分析物存在下形成第二构象，以及与探针相关联的标记，其产生指示探针构象的信号。 描述了用于检测与附着到杂交开关探针的结合配偶体形成特异性结合对的分析物的方法，从而将探针从第一至第二构象改变，从而导致可检测的信号，其指示分析物在 样品。