公开(公告)号：US09494579B2

公开(公告)日：2016-11-15

申请号：US14481044

申请日：2014-09-09

Applicant: Massachusetts Institute of Technology

Inventor： Eric D. Schwoebel ,  James D. Harper ,  Martha S. Petrovick ,  Frances E. Nargi ,  Todd H. Rider ,  Kristine E. Hogan ,  Richard H. Mathews ,  Joseph Lacirignola ,  Mark Hennessy ,  Trina R. Vian ,  Rose M. Joseph ,  Raymond S. Uttaro ,  Shaun Berry ,  Bernadette Johnson ,  Mark A. Hollis

IPC: C12M1/34 ,  C12M3/00 ,  G01N33/53 ,  C12Q1/68 ,  G01N21/64 ,  G01N33/58

CPC classification number: G01N33/5304 ,  C12M1/3461 ,  C12M1/3476 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/582 ,  C12Q2565/619 ,  C12Q2563/131

Abstract: The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor cell comprising an Fc receptor and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more antibodies. Also provided is an optoelectronic sensor device for detecting a target particle in a plurality of samples.

Abstract translation: 本文描述的本发明提供了用于检测可溶性抗原的方法。 特别地，该方法提供了可溶性蛋白质和化学物质的检测。 此外，本发明提供了检测样品中核酸序列的方法。 还描述了包含Fc受体和发射体分子的发射体细胞，用于检测样品中的靶颗粒，其中待检测的靶颗粒被一种或多种抗体结合。 还提供了一种用于检测多个样品中的目标颗粒的光电传感器装置。

公开(公告)号：US20150093745A1

公开(公告)日：2015-04-02

申请号：US14540273

申请日：2014-11-13

Applicant: Massachusetts Institute of Technology

Inventor： James Douglas Harper ,  Richard Hart Mathews ,  Bernadette Johnson ,  Martha Susan Petrovick ,  Ann Rundell ,  Frances Ellen Nargi ,  Timothy Stephens ,  Linda Marie Mendenhall ,  Mark Alexander Hollis ,  Albert M. Young ,  Todd H. Rider ,  Eric David Schwoebel ,  Trina Rae Vian

IPC: G01N21/64 ,  G01N33/569 ,  G01N21/75 ,  G01N15/02

CPC classification number: G01N33/554 ,  G01N15/0205 ,  G01N21/07 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/75 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/54393 ,  G01N33/56983 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2201/025 ,  G01N2201/061 ,  Y10S435/808 ,  Y10S436/805

Abstract: The invention relates to optoelectronic systems for detecting one or more target particles. The system includes a reaction chamber, a specimen collector, an optical detector, and a reservoir containing cells, each of the cells having receptors which are present on the surface of each cell and are specific for the target particle to be detected, where binding of the target particle to the receptors directly or indirectly activates a reporter molecule, thereby producing a measurable optical signal.

Abstract translation: 本发明涉及用于检测一个或多个目标颗粒的光电子系统。 该系统包括反应室，样品收集器，光学检测器和含有细胞的储存器，每个细胞具有存在于每个细胞表面上并且对待检测的靶颗粒是特异性的受体，其中结合 受体的目标颗粒直接或间接激活报告分子，从而产生可测量的光信号。

公开(公告)号：US20150050723A1

公开(公告)日：2015-02-19

申请号：US14481044

申请日：2014-09-09

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： Eric D. Schwoebel ,  James D. Harper ,  Martha S. Petrovick ,  Frances E. Nargi ,  Todd H. Rider ,  Kristine E. Hogan ,  Richard H. Mathews ,  Joseph Lacirignola ,  Mark Hennessy ,  Trina R. Vian ,  Rose M. Joseph ,  Raymond S. Uttaro ,  Shaun Berry ,  Bernadette Johnson ,  Mark A. Hollis

IPC: G01N33/53

CPC classification number: G01N33/5304 ,  C12M1/3461 ,  C12M1/3476 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/582 ,  C12Q2565/619 ,  C12Q2563/131

Abstract: The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor cell comprising an Fc receptor and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more antibodies. Also provided is an optoelectronic sensor device for detecting a target particle in a plurality of samples.

Abstract translation: 本文描述的本发明提供了用于检测可溶性抗原的方法。 特别地，该方法提供了可溶性蛋白质和化学物质的检测。 此外，本发明提供了检测样品中核酸序列的方法。 还描述了包含Fc受体和发射体分子的发射体细胞，用于检测样品中的靶颗粒，其中待检测的靶颗粒被一种或多种抗体结合。 还提供了一种用于检测多个样品中的目标颗粒的光电传感器装置。

公开(公告)号：US20140142026A1

公开(公告)日：2014-05-22

申请号：US14070709

申请日：2013-11-04

Applicant: Massachusetts Institute of Technology

Inventor： Todd H. Rider

IPC: A61K47/48 ,  A61K38/48 ,  A61K38/17 ,  G01N33/569

CPC classification number: A61K38/4873 ,  A61K38/16 ,  A61K38/17 ,  A61K38/1709 ,  A61K47/64 ,  G01N33/5091 ,  G01N33/56983 ,  Y02A50/481

Abstract: Chimeric molecules that contain at least one pathogen-detection domain and at least one effector domain, and their methods of use in preventing or treating a pathogen infection in a cell or organism are described. The pathogen-detection domain and effector domain of the chimeric molecules are domains not typically found in nature to be associated together. Agents are also described herein having at least one pathogen-interacting molecular structure and at least one effector-mediating molecular structure, the agent being one that is non-naturally-occurring in a cell. The methods of prevention and treatment described herein are effective for a broad spectrum of pathogens and exhibit little or no toxic side-effects. Assays for the detection of a pathogen, pathogen component, or product produced or induced by a pathogen, are also provided.

Abstract translation: 描述了含有至少一个病原体检测结构域和至少一个效应子结构域的嵌合分子及其用于预防或治疗细胞或生物体中的病原体感染的方法。 嵌合分子的病原体检测结构域和效应子结构域是自然界中通常不被结合在一起的结构域。 本文还描述了具有至少一种病原体相互作用的分子结构和至少一种效应物介导分子结构的试剂，所述试剂是在细胞中非天然存在的试剂。 本文所述的预防和治疗方法对于广谱的病原体是有效的，并且表现出很少的或没有毒性的副作用。 还提供了用于检测病原体，病原体成分​​或由病原体产生或诱导的产物的检测。

公开(公告)号：US20160161479A1

公开(公告)日：2016-06-09

申请号：US14538271

申请日：2014-11-11

Applicant: Massachusetts Institute of Technology

Inventor： James Douglas Harper ,  Richard Hart Mathews ,  Bernadette Johnson ,  Martha Susan Petrovick ,  Ann Rundell ,  Frances Ellen Nargi ,  Timothy Stephens ,  Linda Marie Mendenhall ,  Mark Alexander Hollis ,  Albert M. Young ,  Todd H. Rider ,  Eric David Schwoebel ,  Trina Rae Vian

IPC: G01N33/554 ,  G01N33/543 ,  G01N21/07 ,  G01N21/76 ,  G01N21/64

CPC classification number: G01N33/554 ,  G01N15/0205 ,  G01N21/07 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/75 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/54393 ,  G01N33/56983 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2201/025 ,  G01N2201/061 ,  Y10S435/808 ,  Y10S436/805

Abstract: The invention relates to optoelectronic systems for detecting one or more target particles. The system includes a reaction chamber, a specimen collector, an optical detector, and a reservoir containing cells, each of the cells having receptors which are present on the surface of each cell and are specific for the target particle to be detected, where binding of the target particle to the receptors directly or indirectly activates a reporter molecule, thereby producing a measurable optical signal.