公开(公告)号：US10995367B2

公开(公告)日：2021-05-04

申请号：US16576582

申请日：2019-09-19

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6853 ,  C12N15/66 ,  C12Q1/6806 ,  C40B40/08 ,  C40B50/06 ,  B01J19/00 ,  C12N15/10 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/686 ,  C40B60/14 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12N9/22 ,  C12N9/00 ,  C12N11/06 ,  C40B50/14

Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

公开(公告)号：US10544451B2

公开(公告)日：2020-01-28

申请号：US15510890

申请日：2014-11-21

Applicant: MGI Tech Co., Ltd.

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6853 ,  C12N15/66 ,  C12Q1/6806 ,  C40B40/08 ,  C40B50/06 ,  B01J19/00 ,  C12N15/10 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/686 ,  C40B60/14 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12N9/22 ,  C12N9/00 ,  C12N11/06 ,  C40B50/14

Abstract: Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

公开(公告)号：US20200080140A1

公开(公告)日：2020-03-12

申请号：US16576582

申请日：2019-09-19

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6853 ,  C40B50/14 ,  C12N15/10 ,  C12N11/06 ,  C12N9/00 ,  C12N9/22 ,  C12Q1/6874 ,  C12Q1/6855 ,  C40B60/14 ,  C12Q1/686 ,  C12Q1/6837 ,  C12Q1/6811 ,  B01J19/00 ,  C40B50/06 ,  C40B40/08 ,  C12Q1/6806 ,  C12N15/66

Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

公开(公告)号：US10316356B1

公开(公告)日：2019-06-11

申请号：US15528353

申请日：2014-11-21

Applicant: MGI Tech Co., Ltd.

Inventor： Yuan Jiang ,  Kai Tian ,  Xia Zhao ,  Wenwei Zhang ,  Huaiqian Xu ,  Hui Jiang ,  Radoje Drmanac ,  Chunyu Geng

IPC: C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3′-terminal and the 5′-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3′-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.