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    PCR primer pair and application thereof
    2.
    发明授权
    PCR primer pair and application thereof 有权

    公开(公告)号：US11739319B2

    公开(公告)日：2023-08-29

    申请号：US16624779

    申请日：2017-06-20

    Applicant: MGI TECH CO., LTD.

    Inventor： Lin Yang ,  Guodong Huang ,  Ya Gao ,  Yanyan Zhang ,  Haiping Zhang ,  Fang Chen ,  Haojun Jiang ,  Shaohong Zhang ,  Yuqian Wang ,  Yifan Xie ,  Hui Jiang

    IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/10 ,  C12N15/11

    CPC classification number: C12N15/1093 ,  C12N15/11

    Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3′ of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.

    RANDOM EMULSIFICATION DIGITAL ABSOLUTE QUANTITATIVE ANALYSIS METHOD AND DEVICE
    4.
    发明申请
    RANDOM EMULSIFICATION DIGITAL ABSOLUTE QUANTITATIVE ANALYSIS METHOD AND DEVICE 有权

    公开(公告)号：US20220411858A1

    公开(公告)日：2022-12-29

    申请号：US17756625

    申请日：2019-11-29

    Applicant: MGI TECH CO., LTD.

    Inventor： Yun Xia ,  Xia Zhao ,  Yang Xi ,  Fang Chen ,  Hui Jiang

    IPC: C12Q1/6851

    Abstract: A random emulsification digital absolute quantitative analysis method includes: performing random emulsification processing on a system to be emulsified to obtain several isolated reaction zones or droplets; determining the total number and volume information of the various reaction zones or droplets, the presence of target molecules to be tested in the respective reaction zones or droplets, and the number of reaction zones or droplets which do not contain the target molecules by combining acquired target images comprising image regions corresponding to the amplified reaction zones or droplets, and analyzing the target images; and accurately calculating the volume information of the various reaction zones or droplets, the presence of the target molecules to be tested in the respective reaction zones or droplets, and the number of reaction zones or droplets which do not contain the target molecules, the total number of target molecules in a sample to be tested.

    PCR primer pair and application thereof
    6.
    发明授权
    PCR primer pair and application thereof 有权

    公开(公告)号：US12104205B2

    公开(公告)日：2024-10-01

    申请号：US16624782

    申请日：2017-06-20

    Applicant: MGI Tech Co., Ltd.

    Inventor： Lin Yang ,  Haojun Jiang ,  Peng Zeng ,  Xuehan Zhuang ,  Ya Gao ,  Yanyan Zhang ,  Hui Jiang ,  Jing Guo ,  Fang Chen ,  Xun Xu

    IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6853 ,  C12Q1/686

    CPC classification number: C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6853 ,  C12Q2600/16

    Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3′ end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.

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