公开(公告)号：US20210164042A1

公开(公告)日：2021-06-03

申请号：US17172927

申请日：2021-02-10

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Xia Zhao ,  Qiaoling Li ,  Shengmao Liu ,  Bo Wang ,  Li Chen ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6874 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

公开(公告)号：US20200080140A1

公开(公告)日：2020-03-12

申请号：US16576582

申请日：2019-09-19

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6853 ,  C40B50/14 ,  C12N15/10 ,  C12N11/06 ,  C12N9/00 ,  C12N9/22 ,  C12Q1/6874 ,  C12Q1/6855 ,  C40B60/14 ,  C12Q1/686 ,  C12Q1/6837 ,  C12Q1/6811 ,  B01J19/00 ,  C40B50/06 ,  C40B40/08 ,  C12Q1/6806 ,  C12N15/66

Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

公开(公告)号：US11827933B2

公开(公告)日：2023-11-28

申请号：US17172927

申请日：2021-02-10

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Xia Zhao ,  Qiaoling Li ,  Shengmao Liu ,  Bo Wang ,  Li Chen ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6874 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855

CPC classification number: C12Q1/6874 ,  C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2525/301 ,  C12Q2535/122

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

公开(公告)号：US10351848B2

公开(公告)日：2019-07-16

申请号：US15510882

申请日：2014-11-26

Applicant: MGI TECH CO., LTD.

Inventor： Chunyu Geng ,  Ruoying Chen ,  Yuan Jiang ,  Xia Zhao ,  Rongrong Guo ,  Lingyu He ,  Yaqiao Li ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12N15/66

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

公开(公告)号：US11680285B2

公开(公告)日：2023-06-20

申请号：US16762898

申请日：2017-11-09

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Yang Xi ,  Wenwei Zhang ,  Pengjuan Liu ,  Xia Zhao ,  Qiaoling Li ,  Hanjie Shen ,  Yongwei Zhang ,  Radoje Drmanac

IPC: C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6876

CPC classification number: C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6876

Abstract: Provided are a hooked probe, a method for ligating a nucleic acid and a method for constructing a sequencing library. The hooked probe comprises a target specific area and a hooked area ligated thereto; the target specific area comprises a sequence complementarily paired with at least part of the single chain of the nucleic acid fragment to be ligated; the hooked area comprises a sequence unpaired with the nucleic acid fragment; the end of the hooked area is a ligatable end; and the ligatable end can ligate the end of the single chain of the nucleic acid fragment.

公开(公告)号：US10316356B1

公开(公告)日：2019-06-11

申请号：US15528353

申请日：2014-11-21

Applicant: MGI Tech Co., Ltd.

Inventor： Yuan Jiang ,  Kai Tian ,  Xia Zhao ,  Wenwei Zhang ,  Huaiqian Xu ,  Hui Jiang ,  Radoje Drmanac ,  Chunyu Geng

IPC: C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3′-terminal and the 5′-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3′-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

公开(公告)号：US10995367B2

公开(公告)日：2021-05-04

申请号：US16576582

申请日：2019-09-19

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6853 ,  C12N15/66 ,  C12Q1/6806 ,  C40B40/08 ,  C40B50/06 ,  B01J19/00 ,  C12N15/10 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/686 ,  C40B60/14 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12N9/22 ,  C12N9/00 ,  C12N11/06 ,  C40B50/14

Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

公开(公告)号：US10954559B2

公开(公告)日：2021-03-23

申请号：US15528362

申请日：2014-11-21

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Xia Zhao ,  Qiaoling Li ,  Shengmao Liu ,  Bo Wang ,  Li Chen ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6874

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

公开(公告)号：US10544451B2

公开(公告)日：2020-01-28

申请号：US15510890

申请日：2014-11-21

Applicant: MGI Tech Co., Ltd.

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6853 ,  C12N15/66 ,  C12Q1/6806 ,  C40B40/08 ,  C40B50/06 ,  B01J19/00 ,  C12N15/10 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/686 ,  C40B60/14 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12N9/22 ,  C12N9/00 ,  C12N11/06 ,  C40B50/14

Abstract: Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.