公开(公告)号：US10316356B1

公开(公告)日：2019-06-11

申请号：US15528353

申请日：2014-11-21

Applicant: MGI Tech Co., Ltd.

Inventor： Yuan Jiang ,  Kai Tian ,  Xia Zhao ,  Wenwei Zhang ,  Huaiqian Xu ,  Hui Jiang ,  Radoje Drmanac ,  Chunyu Geng

IPC: C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3′-terminal and the 5′-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3′-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

公开(公告)号：US20210164042A1

公开(公告)日：2021-06-03

申请号：US17172927

申请日：2021-02-10

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Xia Zhao ,  Qiaoling Li ,  Shengmao Liu ,  Bo Wang ,  Li Chen ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6874 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

公开(公告)号：US20200080140A1

公开(公告)日：2020-03-12

申请号：US16576582

申请日：2019-09-19

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6853 ,  C40B50/14 ,  C12N15/10 ,  C12N11/06 ,  C12N9/00 ,  C12N9/22 ,  C12Q1/6874 ,  C12Q1/6855 ,  C40B60/14 ,  C12Q1/686 ,  C12Q1/6837 ,  C12Q1/6811 ,  B01J19/00 ,  C40B50/06 ,  C40B40/08 ,  C12Q1/6806 ,  C12N15/66

Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

公开(公告)号：US11827933B2

公开(公告)日：2023-11-28

申请号：US17172927

申请日：2021-02-10

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Xia Zhao ,  Qiaoling Li ,  Shengmao Liu ,  Bo Wang ,  Li Chen ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6874 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855

CPC classification number: C12Q1/6874 ,  C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2525/301 ,  C12Q2535/122

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

公开(公告)号：US10351848B2

公开(公告)日：2019-07-16

申请号：US15510882

申请日：2014-11-26

Applicant: MGI TECH CO., LTD.

Inventor： Chunyu Geng ,  Ruoying Chen ,  Yuan Jiang ,  Xia Zhao ,  Rongrong Guo ,  Lingyu He ,  Yaqiao Li ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12N15/66

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

公开(公告)号：US20230265500A1

公开(公告)日：2023-08-24

申请号：US18067540

申请日：2022-12-16

Applicant: MGI Tech Co., Ltd.

Inventor： Xia Zhao ,  Hanjie SHEN ,  Pengjuan LIU ,  Qiaoling LI ,  Yang Xi ,  Yuan JIANG ,  Fang Chen ,  Hui Jiang

IPC: C12Q1/6869 ,  C12N15/10 ,  C12Q1/6855

CPC classification number: C12Q1/6869 ,  C12N15/1093 ,  C12Q1/6855

Abstract: Provided is a PCR-free library construction and sequencing method. A PCR-free high-throughput sequencing method is provided, including the following steps: obtaining a DNA fragment of target size by performing or not performing, based on a size of a nucleic acid sample, fragmentation on the nucleic acid sample; performing end repair and an A-tailing reaction; ligating an adapter containing a barcode; obtaining DNA nanoballs by performing single-strand cyclization and rolling circle replication; and loading and sequencing.

公开(公告)号：US11680285B2

公开(公告)日：2023-06-20

申请号：US16762898

申请日：2017-11-09

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Yang Xi ,  Wenwei Zhang ,  Pengjuan Liu ,  Xia Zhao ,  Qiaoling Li ,  Hanjie Shen ,  Yongwei Zhang ,  Radoje Drmanac

IPC: C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6876

CPC classification number: C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6876

Abstract: Provided are a hooked probe, a method for ligating a nucleic acid and a method for constructing a sequencing library. The hooked probe comprises a target specific area and a hooked area ligated thereto; the target specific area comprises a sequence complementarily paired with at least part of the single chain of the nucleic acid fragment to be ligated; the hooked area comprises a sequence unpaired with the nucleic acid fragment; the end of the hooked area is a ligatable end; and the ligatable end can ligate the end of the single chain of the nucleic acid fragment.

公开(公告)号：US20220411858A1

公开(公告)日：2022-12-29

申请号：US17756625

申请日：2019-11-29

Applicant: MGI TECH CO., LTD.

Inventor： Yun Xia ,  Xia Zhao ,  Yang Xi ,  Fang Chen ,  Hui Jiang

IPC: C12Q1/6851

Abstract: A random emulsification digital absolute quantitative analysis method includes: performing random emulsification processing on a system to be emulsified to obtain several isolated reaction zones or droplets; determining the total number and volume information of the various reaction zones or droplets, the presence of target molecules to be tested in the respective reaction zones or droplets, and the number of reaction zones or droplets which do not contain the target molecules by combining acquired target images comprising image regions corresponding to the amplified reaction zones or droplets, and analyzing the target images; and accurately calculating the volume information of the various reaction zones or droplets, the presence of the target molecules to be tested in the respective reaction zones or droplets, and the number of reaction zones or droplets which do not contain the target molecules, the total number of target molecules in a sample to be tested.

公开(公告)号：US10995367B2

公开(公告)日：2021-05-04

申请号：US16576582

申请日：2019-09-19

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/6853 ,  C12N15/66 ,  C12Q1/6806 ,  C40B40/08 ,  C40B50/06 ,  B01J19/00 ,  C12N15/10 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/686 ,  C40B60/14 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12N9/22 ,  C12N9/00 ,  C12N11/06 ,  C40B50/14

Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

公开(公告)号：US10954559B2

公开(公告)日：2021-03-23

申请号：US15528362

申请日：2014-11-21

Applicant: MGI TECH CO., LTD.

Inventor： Yuan Jiang ,  Xia Zhao ,  Qiaoling Li ,  Shengmao Liu ,  Bo Wang ,  Li Chen ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6874

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.