公开(公告)号：US20230265500A1

公开(公告)日：2023-08-24

申请号：US18067540

申请日：2022-12-16

Applicant: MGI Tech Co., Ltd.

Inventor： Xia Zhao ,  Hanjie SHEN ,  Pengjuan LIU ,  Qiaoling LI ,  Yang Xi ,  Yuan JIANG ,  Fang Chen ,  Hui Jiang

IPC: C12Q1/6869 ,  C12N15/10 ,  C12Q1/6855

CPC classification number: C12Q1/6869 ,  C12N15/1093 ,  C12Q1/6855

Abstract: Provided is a PCR-free library construction and sequencing method. A PCR-free high-throughput sequencing method is provided, including the following steps: obtaining a DNA fragment of target size by performing or not performing, based on a size of a nucleic acid sample, fragmentation on the nucleic acid sample; performing end repair and an A-tailing reaction; ligating an adapter containing a barcode; obtaining DNA nanoballs by performing single-strand cyclization and rolling circle replication; and loading and sequencing.

公开(公告)号：US20210172009A1

公开(公告)日：2021-06-10

申请号：US16762898

申请日：2017-11-09

Applicant: MGI TECH CO., LTD.

Inventor： Yuan JIANG ,  Yang XI ,  Wenwei ZHANG ,  Pengjuan LIU ,  Xia ZHAO ,  Qiaoling LI ,  Hanjie SHEN ,  Yongwei ZHANG ,  Radoje DRMANAC

IPC: C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6876

Abstract: Provided are a hooked probe, a method for ligating a nucleic acid and a method for constructing a sequencing library. The hooked probe comprises a target specific area and a hooked area ligated thereto; the target specific area comprises a sequence complementarily paired with at least part of the single chain of the nucleic acid fragment to be ligated; the hooked area comprises a sequence unpaired with the nucleic acid fragment; the end of the hooked area is a ligatable end; and the ligatable end can ligate the end of the single chain of the nucleic acid fragment.