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公开(公告)号：US11739319B2
公开(公告)日：2023-08-29
申请号：US16624779
申请日：2017-06-20
Applicant: MGI TECH CO., LTD.
Inventor： Lin Yang , Guodong Huang , Ya Gao , Yanyan Zhang , Haiping Zhang , Fang Chen , Haojun Jiang , Shaohong Zhang , Yuqian Wang , Yifan Xie , Hui Jiang
CPC classification number: C12N15/1093 , C12N15/11
Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3′ of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.
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公开(公告)号：US20230265500A1
公开(公告)日：2023-08-24
申请号：US18067540
申请日：2022-12-16
Applicant: MGI Tech Co., Ltd.
Inventor： Xia Zhao , Hanjie SHEN , Pengjuan LIU , Qiaoling LI , Yang Xi , Yuan JIANG , Fang Chen , Hui Jiang
IPC: C12Q1/6869 , C12N15/10 , C12Q1/6855
CPC classification number: C12Q1/6869 , C12N15/1093 , C12Q1/6855
Abstract: Provided is a PCR-free library construction and sequencing method. A PCR-free high-throughput sequencing method is provided, including the following steps: obtaining a DNA fragment of target size by performing or not performing, based on a size of a nucleic acid sample, fragmentation on the nucleic acid sample; performing end repair and an A-tailing reaction; ligating an adapter containing a barcode; obtaining DNA nanoballs by performing single-strand cyclization and rolling circle replication; and loading and sequencing.
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公开(公告)号：US20220411858A1
公开(公告)日：2022-12-29
申请号：US17756625
申请日：2019-11-29
Applicant: MGI TECH CO., LTD.
IPC: C12Q1/6851
Abstract: A random emulsification digital absolute quantitative analysis method includes: performing random emulsification processing on a system to be emulsified to obtain several isolated reaction zones or droplets; determining the total number and volume information of the various reaction zones or droplets, the presence of target molecules to be tested in the respective reaction zones or droplets, and the number of reaction zones or droplets which do not contain the target molecules by combining acquired target images comprising image regions corresponding to the amplified reaction zones or droplets, and analyzing the target images; and accurately calculating the volume information of the various reaction zones or droplets, the presence of the target molecules to be tested in the respective reaction zones or droplets, and the number of reaction zones or droplets which do not contain the target molecules, the total number of target molecules in a sample to be tested.
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Patent Agency Ranking
公开(公告)号：US11242520B2
公开(公告)日：2022-02-08
申请号：US16519671
申请日：2019-07-23
Applicant: BGI Shenzhen , MGI Tech Co., Ltd.
IPC: C12Q1/6806 , C12N15/10 , G01N35/00
Abstract: Provided is a method for isolating exosomal DNA, the method comprising: (i) providing a sample, wherein the sample comprises a blood sample from peripheral blood of a pregnant woman; and (ii) subjecting the sample to isolating, thus obtaining the exosomal DNA. Also provided are a method for detecting a blood sample, a method for constructing a sequencing library on a blood sample, a method for high-throughput sequencing an exosomal DNA sequencing library, a method for non-invasive prenatal gene detection, a device for non-invasive prenatal gene detection, and a kit for detecting a blood sample and use thereof.
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公开(公告)号：US12104205B2
公开(公告)日：2024-10-01
申请号：US16624782
申请日：2017-06-20
Applicant: MGI Tech Co., Ltd.
Inventor： Lin Yang , Haojun Jiang , Peng Zeng , Xuehan Zhuang , Ya Gao , Yanyan Zhang , Hui Jiang , Jing Guo , Fang Chen , Xun Xu
IPC: C12Q1/68 , C12Q1/6818 , C12Q1/6853 , C12Q1/686
CPC classification number: C12Q1/686 , C12Q1/6818 , C12Q1/6853 , C12Q2600/16
Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3′ end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.
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公开(公告)号：US11760995B2
公开(公告)日：2023-09-19
申请号：US16624791
申请日：2017-06-20
Applicant: MGI TECH CO., LTD
Inventor： Lin Yang , Ya Gao , Guodong Huang , Yicong Wang , Yuqian Wang , Yanyan Zhang , Fang Chen , Na Zhong , Hui Jiang , Xun Xu
IPC: C12N15/10 , C12Q1/68 , C12Q1/6853 , C12Q1/686
CPC classification number: C12N15/1093 , C12Q1/686 , C12Q1/6853
Abstract: Provided is a PCR primer pair, comprising: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on 3′ end of the first primer, and the first random sequence is located on 5′ end of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on 3′ end of the second primer, and the second random sequence is located on 5′ end of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence respectively, and the first random sequence and the second random sequence are inverse complementary.
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公开(公告)号：US20200216874A1
公开(公告)日：2020-07-09
申请号：US16624782
申请日：2017-06-20
Applicant: MGI Tech Co., Ltd.
Inventor： Lin Yang , Haojun Jiang , Peng Zeng , Xuehan Zhuang , Ya Gao , Yanyan Zhang , Hui Jiang , Jing Guo , Fang Chen , Xun Xu
IPC: C12Q1/686 , C12Q1/6818 , C12Q1/6853
Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3′ end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.
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公开(公告)号：US20200216838A1
公开(公告)日：2020-07-09
申请号：US16624779
申请日：2017-06-20
Applicant: MGI TECH CO., LTD.
Inventor： Lin Yang , Guodong Huang , Ya Gao , Yanyan Zhang , Haiping Zhang , Fang Chen , Haojun Jiang , Shaohong Zhang , Yuqian Wang , Yifan Xie , Hui Jiang
Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3′ of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.
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