公开(公告)号：US11732268B2

公开(公告)日：2023-08-22

申请号：US16311749

申请日：2017-06-27

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Elysia K. Krieger ,  Richard J. Lawrence ,  Zarir E. Vaghchhipawala ,  Jianping Xu ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8202 ,  C12N15/8205 ,  C12N15/8213

Abstract: The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.

公开(公告)号：US20210054390A1

公开(公告)日：2021-02-25

申请号：US17045747

申请日：2019-05-24

Applicant: Monsanto Technology LLC

Inventor： Yurong Chen ,  Annie Saltarikos ,  Jianping Xu ,  Xudong Ye

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/113

Abstract: Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

公开(公告)号：US11162109B2

公开(公告)日：2021-11-02

申请号：US16221346

申请日：2018-12-14

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Larry A. Gilbertson ,  Jon Lamb ,  Jason Ward ,  Jianping Xu

IPC: C12N15/82

Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

公开(公告)号：US11807861B2

公开(公告)日：2023-11-07

申请号：US17469487

申请日：2021-09-08

Applicant: Monsanto Technology LLC

Inventor： Larry Gilbertson ,  Jon Lamb ,  Jason Ward ,  Jianping Xu

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8216

Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

公开(公告)号：US20220064659A1

公开(公告)日：2022-03-03

申请号：US17469487

申请日：2021-09-08

Applicant: Monsanto Technology LLC

Inventor： Larry Gilbertson ,  Jon Lamb ,  Jason Ward ,  Jianping Xu

IPC: C12N15/82

Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

公开(公告)号：US10179916B2

公开(公告)日：2019-01-15

申请号：US14516483

申请日：2014-10-16

Applicant: Monsanto Technology LLC

Inventor： Larry Gilbertson ,  Jason Ward ,  Jianping Xu ,  Jon Lamb

IPC: C12N15/82

Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

公开(公告)号：US20150040270A1

公开(公告)日：2015-02-05

申请号：US14516483

申请日：2014-10-16

Applicant: Monsanto Technology LLC

Inventor： Larry Gilbertson ,  Jason Ward ,  Jianping Xu ,  Jon Lamb

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8216

Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

Abstract translation: 提供包含单个基因座中包含的多个转基因的转基因植物。 在某些方面，可以从第一位点表达7个或更多个转基因。 提供了用于转化具有多个转基因的植物细胞的方法。 还提供了用于表达和增强植物中一种或多种转基因表达的方法。

公开(公告)号：US20190194681A1

公开(公告)日：2019-06-27

申请号：US16221346

申请日：2018-12-14

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Larry A. Gilbertson ,  Jonathan C. Lamb ,  Jason Ward ,  Jianping Xu

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8216

Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.