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    GENOME EDITING IN PLANTS
    4.
    发明申请
    GENOME EDITING IN PLANTS 有权

    公开(公告)号：US20210054390A1

    公开(公告)日：2021-02-25

    申请号：US17045747

    申请日：2019-05-24

    Applicant: Monsanto Technology LLC

    Inventor： Yurong Chen ,  Annie Saltarikos ,  Jianping Xu ,  Xudong Ye

    IPC: C12N15/82 ,  C12N9/22 ,  C12N15/113

    Abstract: Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

    COMPOSITIONS AND METHODS FOR TRANSFORMATION OF EMBRYO EXPLANT POPULATIONS
    5.
    发明公开
    COMPOSITIONS AND METHODS FOR TRANSFORMATION OF EMBRYO EXPLANT POPULATIONS 审中-公开

    公开(公告)号：US20240110194A1

    公开(公告)日：2024-04-04

    申请号：US18377421

    申请日：2023-10-06

    Applicant: Monsanto Technology LLC

    Inventor： Brent Brower-Toland ,  David Vincent Butruille ,  Edward J. Cargill ,  Yurong Chen ,  Megan Elizabeth Hassebrock ,  Thomas Ream ,  Jennifer Rinehart ,  Mary Ann Saltarikos ,  Michelle Folta Valentine

    IPC: C12N15/82 ,  C12N9/22 ,  C12N15/11

    CPC classification number: C12N15/8205 ,  C12N9/22 ,  C12N15/11

    Abstract: The present disclosure provides novel compositions and methods for collectively transforming or genetically modifying a population of distinct germplasm of different germplasms or having different genotypes. The compositions of the present disclosure may include a population of distinct germplasm, such as embryo explants, and a heterologous polynucleotide molecule, a ribonucleoprotein, or a site-specific nuclease. The methods of the present disclosure may include one or more steps of explant preparation, explant rehydration, Rhizobiales bacterium inoculation and co-culture or particle bombardment, bud induction, extended bud induction, and/or regeneration or development of genetically modified plants or plant parts. The methods provided herein may include transforming at least one plant cell of the embryo explants with a heterologous polynucleotide. The methods provided herein also include methods of regenerating or growing a plurality of genetically modified plants or plant parts from the population of transformed or edited plant cells or explants and comparing, selecting or screening one or more genotypes having an improved phenotype or other culturing characteristic.

    GENOME EDITING IN PLANTS
    8.
    发明申请
    GENOME EDITING IN PLANTS 有权

    公开(公告)号：US20210238613A1

    公开(公告)日：2021-08-05

    申请号：US17049326

    申请日：2019-05-24

    Applicant: Monsanto Technology LLC

    Inventor： Yurong Chen ,  Annie Saltarikos ,  Dafu Wang

    IPC: C12N15/82

    Abstract: Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated or applied with a nuclease protein, guide RNA or RNP for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

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