INDUCED SPORULATION SCREENING METHOD
    1.
    发明申请
    INDUCED SPORULATION SCREENING METHOD 审中-公开
    诱导运动筛选方法

    公开(公告)号:US20130224757A1

    公开(公告)日:2013-08-29

    申请号:US13816891

    申请日:2011-08-18

    IPC分类号: C12Q1/68

    CPC分类号: C12Q1/6897 C12Q1/025

    摘要: The present invention relates to a method of screening for an improved enzyme variant, said method comprising the steps of: (i) providing a recombinant host cell capable of sporulating comprising a polynucleotide encoding a sporulation factor and a polynucleotide encoding an enzyme variant which are operably linked to an inducible promoter; (ii) culturing the host cell under conditions suitable to induce the formation of spores; (iii) culturing the spores obtained in step (ii) in a medium containing a substrate for the enzyme variant; and (iv) determining the activity of the enzyme variant.

    摘要翻译: 本发明涉及一种筛选改良酶变体的方法,所述方法包括以下步骤:(i)提供能够进行孢子形成的重组宿主细胞,其包含编码孢子形成因子的多核苷酸和编码酶变体的多核苷酸,其可操作地 与诱导型启动子连接; (ii)在适合诱导孢子形成的条件下培养宿主细胞; (iii)将培养步骤(ii)中获得的孢子培养在含有酶变体底物的培养基中; 和(iv)确定酶变体的活性。