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公开(公告)号：US09434932B2
公开(公告)日：2016-09-06
申请号：US14129565
申请日：2012-06-29
申请人： Svend Kaasgaard , Jens Oebro , Signe Eskildsen Larsen , Allan Svendsen , Annette Helle Johansen , Michael Skjoet , Carsten Andersen , Lars Beier , Esben Peter Friis , Miguel Duarte Guilherme Pereira Toscano , Mads Bjoernvad , Frank Winther Rasmussen , Liv Spaanger Christiansen
发明人： Svend Kaasgaard , Jens Oebro , Signe Eskildsen Larsen , Allan Svendsen , Annette Helle Johansen , Michael Skjoet , Carsten Andersen , Lars Beier , Esben Peter Friis , Miguel Duarte Guilherme Pereira Toscano , Mads Bjoernvad , Frank Winther Rasmussen , Liv Spaanger Christiansen
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , C12Y302/01001
摘要： The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
摘要翻译： 本发明涉及母体α-淀粉酶的变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。
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2.发明申请CLEANING COMPOSITIONS COMPRISING AMYLASE VARIANTS REFERENCE TO A SEQUENCE LISTING 有权包含氨基酸变体的清洁组合物参考序列表公开(公告)号：US20130000055A1
公开(公告)日：2013-01-03
申请号：US13537606
申请日：2012-06-29
申请人： Michelle Jackson , Philip Frank Souter , Lindsay Suzanne Bewick , Svend Kaasgaard , Jens Oebro , Signe Eskildsen Larsen , Allan Svendsen , Annette Helle Johansen , Michael Skjoet , Carsten Andersen , Lars Beier , Esben Peter Friis , Miguel Duarte Guilherme Pereira Toscano , Mads Bjoernvad , Frank Winther Rasmussen , Liv Spaangner Christiansen
发明人： Michelle Jackson , Philip Frank Souter , Lindsay Suzanne Bewick , Svend Kaasgaard , Jens Oebro , Signe Eskildsen Larsen , Allan Svendsen , Annette Helle Johansen , Michael Skjoet , Carsten Andersen , Lars Beier , Esben Peter Friis , Miguel Duarte Guilherme Pereira Toscano , Mads Bjoernvad , Frank Winther Rasmussen , Liv Spaangner Christiansen
CPC分类号： C11D3/386 , C11D3/38681 , C12N9/2417 , C12Y302/01001
摘要： The present invention relates to cleaning compositions comprising variants of an alpha-amylase and methods of treating surfaces such as textiles with aqueous liquor comprising such compositions, especially at low temperatures.
摘要翻译： 本发明涉及包含α-淀粉酶的变体的清洁组合物以及包含这种组合物的含水液体的表面如纺织品的处理方法，特别是在低温下。
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3.发明授权Cleaning compositions comprising amylase variants reference to a sequence listing 有权包含淀粉酶变体的清洁组合物参考序列表公开(公告)号：US09284544B2
公开(公告)日：2016-03-15
申请号：US13537606
申请日：2012-06-29
申请人： Michelle Jackson , Philip Frank Souter , Lindsay Suzanne Bewick , Svend Kaasgaard , Jens Oebro , Signe Eskildsen Larsen , Allan Svendsen , Annette Helle Johansen , Michael Skjoet , Carsten Andersen , Lars Beier , Esben Peter Friis , Miguel Duarte Guilherme Pereira Toscano , Mads Bjoernvad , Frank Winther Rasmussen , Liv Spaangner Christiansen
发明人： Michelle Jackson , Philip Frank Souter , Lindsay Suzanne Bewick , Svend Kaasgaard , Jens Oebro , Signe Eskildsen Larsen , Allan Svendsen , Annette Helle Johansen , Michael Skjoet , Carsten Andersen , Lars Beier , Esben Peter Friis , Miguel Duarte Guilherme Pereira Toscano , Mads Bjoernvad , Frank Winther Rasmussen , Liv Spaangner Christiansen
CPC分类号： C11D3/386 , C11D3/38681 , C12N9/2417 , C12Y302/01001
摘要： The present invention relates to cleaning compositions comprising variants of an alpha-amylase and methods of treating surfaces such as textiles with aqueous liquor comprising such compositions, especially at low temperatures.
摘要翻译： 本发明涉及包含α-淀粉酶的变体的清洁组合物和包含这种组合物的含水液体的表面如纺织品的处理方法，特别是在低温下。
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公开(公告)号：US09896673B2
公开(公告)日：2018-02-20
申请号：US13024770
申请日：2011-02-10
申请人： Allan Svendsen , Annette Helle Johansen , Mads Bjoernvad , Frank W. Rasmussen , Michael Skjoet , Signe Eskildsen Larsen , Jens Oebro , Svend Kaasgaard , Lars Beier
发明人： Allan Svendsen , Annette Helle Johansen , Mads Bjoernvad , Frank W. Rasmussen , Michael Skjoet , Signe Eskildsen Larsen , Jens Oebro , Svend Kaasgaard , Lars Beier
CPC分类号： C12N9/2417
摘要： The present invention relates to variants of an alpha-amylase having improved stability to chelating agents relative to its parent enzyme, compositions comprising the variants, nucleic acids encoding the variants, methods of producing the variants, and methods for using the variants.
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5.发明申请Variants And Compositions Comprising Variants With High Stability In Presence Of A Chelating Agent 有权含有高度稳定性的变体和组合物在螯合剂存在下公开(公告)号：US20110195481A1
公开(公告)日：2011-08-11
申请号：US13024770
申请日：2011-02-10
申请人： Allan Svendsen , Annette Helle Johansen , Mads Bjoernvad , Frank W. Rasmussen , Michael Skjoet , Signe Eskildsen Larsen , Jens Oebro , Svend Kaasgaard , Lars Beier
发明人： Allan Svendsen , Annette Helle Johansen , Mads Bjoernvad , Frank W. Rasmussen , Michael Skjoet , Signe Eskildsen Larsen , Jens Oebro , Svend Kaasgaard , Lars Beier
CPC分类号： C12N9/2417
摘要： The present invention relates to variants of an alpha-amylase having improved stability to chelating agents relative to its parent enzyme, compositions comprising the variants, nucleic acids encoding the variants, methods of producing the variants, and methods for using the variants.
摘要翻译： 本发明涉及具有相对于其母体酶对螯合剂具有改善的稳定性的α-淀粉酶的变体，包含变体的组合物，编码变体的核酸，产生变体的方法以及使用该变体的方法。
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公开(公告)号：US20060165613A1
公开(公告)日：2006-07-27
申请号：US10478840
申请日：2002-05-14
申请人： Mads Bjoernvad , Martin Schulein , Torben Henriksen , Vibeke Nielsen , Johan Smets , Carsten Andersen , Thomas Thisted
发明人： Mads Bjoernvad , Martin Schulein , Torben Henriksen , Vibeke Nielsen , Johan Smets , Carsten Andersen , Thomas Thisted
CPC分类号： A61K8/66 , A61Q11/00 , C11D3/38636 , C12N9/88
摘要： Detergent composition comprising a surfactant and a pectate lyase (EC 4.2.2.2) enzyme en-coded by a DNA sequence endogeneous to a strain of Bacillus subtilis or a stabilized variant thereof provides superior cleaning and stain removal.
摘要翻译： 包含表面活性剂和由枯草芽孢杆菌菌株或其稳定变体的内切DNA序列编码的果胶酸裂解酶（EC 4.2.2.2）酶的洗涤剂组合物提供了优异的清洁和去污。
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公开(公告)号：US20060019301A1
公开(公告)日：2006-01-26
申请号：US11186641
申请日：2005-07-20
申请人： Peter Hansen , Mads Bjoernvad , Joel Cherry , Aubrey Jones , Amanda Fischer
发明人： Peter Hansen , Mads Bjoernvad , Joel Cherry , Aubrey Jones , Amanda Fischer
CPC分类号： C40B50/06 , C12N15/102 , C12N15/1082 , C40B40/08
摘要： The present invention relates to methods of producing mutants of a polynucleotide and to mutant polynucleotides and artificial variants encoded by the mutant polynucleotides.
摘要翻译： 本发明涉及产生多核苷酸的突变体的方法和由突变体多核苷酸编码的突变体多核苷酸和人造变体的方法。
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公开(公告)号：US20130224757A1
公开(公告)日：2013-08-29
申请号：US13816891
申请日：2011-08-18
申请人： Mads Bjoernvad , Poul Erik Pedersen , Marco Malten
发明人： Mads Bjoernvad , Poul Erik Pedersen , Marco Malten
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6897 , C12Q1/025
摘要： The present invention relates to a method of screening for an improved enzyme variant, said method comprising the steps of: (i) providing a recombinant host cell capable of sporulating comprising a polynucleotide encoding a sporulation factor and a polynucleotide encoding an enzyme variant which are operably linked to an inducible promoter; (ii) culturing the host cell under conditions suitable to induce the formation of spores; (iii) culturing the spores obtained in step (ii) in a medium containing a substrate for the enzyme variant; and (iv) determining the activity of the enzyme variant.
摘要翻译： 本发明涉及一种筛选改良酶变体的方法，所述方法包括以下步骤：（i）提供能够进行孢子形成的重组宿主细胞，其包含编码孢子形成因子的多核苷酸和编码酶变体的多核苷酸，其可操作地 与诱导型启动子连接; （ii）在适合诱导孢子形成的条件下培养宿主细胞; （iii）将培养步骤（ii）中获得的孢子培养在含有酶变体底物的培养基中; 和（iv）确定酶变体的活性。
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