公开(公告)号：US08211432B2

公开(公告)日：2012-07-03

申请号：US11589760

申请日：2006-10-31

申请人： Magnus Höök ,  Joseph M. Patti ,  Karen L. House-Pompeo ,  Pietro Speziale ,  Danny Joh ,  Martin J. McGavin

发明人： Magnus Höök ,  Joseph M. Patti ,  Karen L. House-Pompeo ,  Pietro Speziale ,  Danny Joh ,  Martin J. McGavin

IPC分类号： A61K39/395 ,  C07K16/18

CPC分类号： A61K39/085 ,  A61K38/00 ,  A61K39/00 ,  A61K39/092 ,  A61K2039/505 ,  C07K14/31 ,  C07K14/315 ,  C07K16/1271 ,  C07K16/1275 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/76 ,  C07K2319/00 ,  G01N33/6887 ,  G01N2333/31 ,  G01N2333/315 ,  G01N2333/78

摘要： Disclosed are antibodies that block the binding of fibronectin protein to fibronectin. Also disclosed are site specifically-mutated and truncated peptide epitopes derived from the fnbA and fnbB genes of Staphylococcus aureus, the fnbA and fnbB genes of Streptococcus dysgalactiae, and the sfb gene of Streptococcus pyogenes, and nucleic acid segments encoding these peptides and epitopes. The anti-(fibronectin binding site) antibodies, peptides and epitopes that give rise to antibodies that block the binding of fibronectin binding proteins to fibronectin, and DNA segments encoding these proteins and are of use in various screening, diagnostic and therapeutic applications including active and passive immunization and methods for the prevention of streptococcal and staphylococcal colonization in animals or humans. These. DNA segments and the peptides derived therefrom are proposed to be of use directly in the preparation of vaccines and also for use as carrier proteins in vaccine formulations.

摘要翻译： 公开了阻断纤连蛋白与纤连蛋白结合的抗体。 还公开了衍生自金黄色葡萄球菌的fnbA和fnbB基因的位点特异性突变和截短的肽表位，失语链球菌的fnbA和fnbB基因，以及化脓性链球菌的sfb基因，以及编码这些肽和表位的核酸片段。 产生阻断纤连蛋白结合蛋白与纤连蛋白结合的抗体的抗（纤连蛋白结合位点）抗体，肽和表位，以及编码这些蛋白质的DNA片段，并且可用于各种筛选，诊断和治疗应用，包括活性和 被动免疫和预防动物或人类中链球菌和葡萄球菌定居的方法。 这些。 提出DNA段及其衍生的肽直接用于疫苗的制备，也可用作疫苗制剂中的载体蛋白。

公开(公告)号：US06685943B1

公开(公告)日：2004-02-03

申请号：US09010317

申请日：1998-01-21

申请人： Magnus Höök ,  Joseph M. Patti ,  Karen L. House-Pompeo ,  Pietro Speziale ,  Danny Joh ,  Martin J. McGavin

发明人： Magnus Höök ,  Joseph M. Patti ,  Karen L. House-Pompeo ,  Pietro Speziale ,  Danny Joh ,  Martin J. McGavin

IPC分类号： A61K3900

CPC分类号： A61K39/085 ,  A61K38/00 ,  A61K39/00 ,  A61K39/092 ,  A61K2039/505 ,  C07K14/31 ,  C07K14/315 ,  C07K16/1271 ,  C07K16/1275 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/76 ,  C07K2319/00 ,  G01N33/6887 ,  G01N2333/31 ,  G01N2333/315 ,  G01N2333/78

摘要： Disclosed are antibodies that block the binding of fibronectin protein to fibronectin. Also disclosed are site specifically-mutated and truncated peptide epitopes derived from the fnbA and fnbB genes of Staphylococcus aureus, the fnba and fnbB genes of Streptococcus dysgalactiae, and the sfb gene of Streptococcus pyogenes, and nucleic acid segments encoding these peptides and epitopes. The anti-(fibronectin binding site) antibodies, peptides and epitopes that give rise to antibodies that block the binding of fibronectin binding proteins to fibronectin, and DNA segments encoding these proteins and are of use in various screening, diagnostic and therapeutic applications including active and passive immunization and methods for the prevention of streptococcal and staphylococcal colonization in animals or humans. These. DNA segments and the peptides derived therefrom are proposed to be of use directly in the preparation of vaccines and also for use as carrier proteins in vaccine formulations.

摘要翻译： 公开了阻断纤连蛋白与纤连蛋白结合的抗体。 还公开了衍生自金黄色葡萄球菌的fnbA和fnbB基因的位点特异性突变和截短的肽表位，来自失血性链球菌的fnba和fnbB基因，以及化脓性链球菌的sfb基因，以及编码这些肽和表位的核酸片段。 产生阻断纤连蛋白结合蛋白与纤连蛋白结合的抗体的抗（纤连蛋白结合位点）抗体，肽和表位，以及编码这些蛋白质的DNA片段，并且可用于各种筛选，诊断和治疗应用，包括活性和 被动免疫和预防动物或人类中链球菌和葡萄球菌定居的方法。 这些。 提出DNA段及其衍生的肽直接用于疫苗的制备，也可用作疫苗制剂中的载体蛋白。

公开(公告)号：US07128912B2

公开(公告)日：2006-10-31

申请号：US10731238

申请日：2003-12-10

申请人： Magnus Höök ,  Joseph M. Patti ,  Karen L. House-Pompeo ,  Pietro Speziale ,  Danny Joh ,  Martin J. McGavin

发明人： Magnus Höök ,  Joseph M. Patti ,  Karen L. House-Pompeo ,  Pietro Speziale ,  Danny Joh ,  Martin J. McGavin

IPC分类号： A61K39/00

CPC分类号： A61K39/085 ,  A61K38/00 ,  A61K39/00 ,  A61K39/092 ,  A61K2039/505 ,  C07K14/31 ,  C07K14/315 ,  C07K16/1271 ,  C07K16/1275 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/76 ,  C07K2319/00 ,  G01N33/6887 ,  G01N2333/31 ,  G01N2333/315 ,  G01N2333/78

摘要： Disclosed are antibodies that block the binding of fibronectin protein to fibronectin. Also disclosed are site specifically-mutated and truncated peptide epitopes derived from the fnbA and fnbB genes of Staphylococcus aureus, the fnbA and fnbB genes of Streptococcus dysgalactiae, and the sfb gene of Streptococcus pyogenes, and nucleic acid segments encoding these peptides and epitopes. The anti-(fibronectin binding site) antibodies, peptides and epitopes that give rise to antibodies that block the binding of fibronectin binding proteins to fibronectin, and DNA segments encoding these proteins and are of use in various screening, diagnostic and therapeutic applications including active and passive immunization and methods for the prevention of streptococcal and staphylococcal colonization in animals or humans. These DNA segments and the peptides derived therefrom are proposed to be of use directly in the preparation of vaccines and also for use as carrier proteins in vaccine formulations.

摘要翻译： 公开了阻断纤连蛋白与纤连蛋白结合的抗体。 还公开了衍生自金黄色葡萄球菌的fnbA和fnbB基因的位点特异性突变和截短的肽表位，失语链球菌的fnbA和fnbB基因，以及化脓性链球菌的sfb基因，以及编码这些肽和表位的核酸片段。 产生阻断纤连蛋白结合蛋白与纤连蛋白结合的抗体的抗（纤连蛋白结合位点）抗体，肽和表位，以及编码这些蛋白质的DNA片段，并且可用于各种筛选，诊断和治疗应用，包括活性和 被动免疫和预防动物或人类中链球菌和葡萄球菌定居的方法。 提出这些DNA片段及其衍生的肽直接用于疫苗的制备，也可用作疫苗制剂中的载体蛋白。

公开(公告)号：US06841154B2

公开(公告)日：2005-01-11

申请号：US10172502

申请日：2002-06-17

申请人： Timothy Foster ,  Fiona Roche ,  Mark Pallen ,  Joseph M. Patti ,  Jeff T. Hutchins ,  Pietro Speziale

发明人： Timothy Foster ,  Fiona Roche ,  Mark Pallen ,  Joseph M. Patti ,  Jeff T. Hutchins ,  Pietro Speziale

IPC分类号： G01N33/53 ,  A61K39/00 ,  A61K39/395 ,  A61K39/40 ,  A61P31/04 ,  C07K14/31 ,  C07K16/12 ,  C12P21/08 ,  G01N33/569

CPC分类号： C07K16/1271

摘要： Polyclonal and monoclonal antibodies which are cross-reactive to both coagulase-positive staphylococcus bacteria, such as S. aureus and to coagulase-negative bacteria, such as S. epidermidis and S. hemolyticus, are provided which can recognize surface proteins from both coagulase-positive and coagulase negative staph bacteria. The antibodies may be generated from surface proteins that have been isolated on the basis of characteristics that may be common between S. aureus and coagulase-negative staphylococci, and these recombinant surface proteins are used to generate the antibodies of the invention. There is also provided vaccines and methods which utilize these proteins and antibodies for the treatment or protection against a wide variety of staphylococcal infections.

摘要翻译： 提供了对凝固酶阳性葡萄球菌细菌如金黄色葡萄球菌和凝血酶阴性细菌如表皮葡萄球菌和溶血性溶血性葡萄球菌交叉反应的多克隆和单克隆抗体，其可以识别来自凝固酶 - 阳性和凝固酶阴性葡萄球菌。 抗体可以从基于在金黄色葡萄球菌和凝固酶阴性葡萄球菌之间常见的特征分离的表面蛋白产生，并且这些重组表面蛋白质用于产生本发明的抗体。 还提供了利用这些蛋白质和抗体用于治疗或保护各种葡萄球菌感染的疫苗和方法。

公开(公告)号：US07968100B2

公开(公告)日：2011-06-28

申请号：US12365481

申请日：2009-02-04

申请人： Timothy Foster ,  Fiona Roche ,  Mark Pallen ,  Joseph M. Patti ,  Jeff T. Hutchins ,  Pietro Speziale

发明人： Timothy Foster ,  Fiona Roche ,  Mark Pallen ,  Joseph M. Patti ,  Jeff T. Hutchins ,  Pietro Speziale

IPC分类号： A61K39/085 ,  A61K38/16 ,  G01N33/569

CPC分类号： C07K16/1271

摘要： Surface proteins are provided which generate polyclonal and monoclonal antibodies which are cross-reactive to both coagulase-positive staphylococcus bacteria, such as S. aureus and to coagulase-negative bacteria, such as S. epidermidis and S. hemolyticus. The antibodies may be generated from surface proteins that have been isolated on the basis of characteristics that may be common between S. aureus and coagulase-negative staphylococci, or the A domains of those surface proteins, and these recombinant surface proteins are used to generate the cross-reactive antibodies. Vaccines comprising an immunologically effective amount of the proteins are also provided, and these vaccines are used in methods for the treatment or protection against a wide variety of staphylococcal infections.

摘要翻译： 提供了表达蛋白，其产生与凝固酶阳性葡萄球菌如金黄色葡萄球菌和凝血酶阴性细菌如表皮葡萄球菌和溶血性葡萄球菌的交叉反应性的多克隆和单克隆抗体。 抗体可以从基于在金黄色葡萄球菌和凝固酶阴性葡萄球菌或这些表面蛋白质的A结构域可能是常见的特征分离的表面蛋白产生，并且这些重组表面蛋白质用于产生 交叉反应性抗体。 还提供了包含免疫有效量的蛋白质的疫苗，并且这些疫苗用于治疗或保护免受各种葡萄球菌感染的方法中。

公开(公告)号：US07241592B2

公开(公告)日：2007-07-10

申请号：US09810428

申请日：2001-03-19

申请人： Magnus Hook ,  Yi Xu ,  Pietro Speziale ,  Livia Visal ,  Fabrizia Casolini ,  Joseph M. Patti ,  Pratiksha Patel ,  Paul Domanski

发明人： Magnus Hook ,  Yi Xu ,  Pietro Speziale ,  Livia Visal ,  Fabrizia Casolini ,  Joseph M. Patti ,  Pratiksha Patel ,  Paul Domanski

IPC分类号： C12P21/08 ,  C12N1/00 ,  A61K39/395 ,  A61K39/40 ,  C07K16/12

CPC分类号： C07K16/1271 ,  A61K39/085 ,  A61K2039/505 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/00 ,  G01N33/56938 ,  G01N2333/31 ,  G01N2333/78 ,  G01N2500/00

摘要： Antibodies to the CNA protein and to other regions from the collagen binding domain, including domain CNA19, are provided, and antibodies produced in this manner have been shown to be cross reactive to both Staphylococcus aureus and Staphylococcus epidermidis bacteria and which can thus be used in the prevention and treatment of infections caused by both of these types of bacteria. In addition, medical instruments can be treated using the antibodies of the invention in order to reduce or eliminate the possibility of their becoming infected or further spreading the infection. In particular, the proteins are advantageous because they are cross-reactive and may thus be administered to patients so as to reduce or prevent severe infection by staphylococcal bacteria of more than one species. Antibodies generated in this manner have also been shown to exhibit displacement activity and can thus be utilized advantageously in methods wherein these antibodies will be administered to patients having pre-existing staphylococcal infections because of the ability to displace bacterial proteins from binding sites on the extracellular matrix. Finally, a method of identifying, isolating and utilizing displacing antibodies is also provided.

摘要翻译： 提供了对CNA蛋白和来自胶原结合结构域的其他区域（包括结构域CNA19）的抗体，并且以这种方式产生的抗体已显示与金黄色葡萄球菌和表皮葡萄球菌交叉反应，因此可以用于 预防和治疗由这两种细菌引起的感染。 此外，可以使用本发明的抗体治疗医疗器械，以便减少或消除它们被感染或进一步扩散感染的可能性。 特别地，蛋白质是有利的，因为它们是交叉反应性的，并且因此可以施用于患者，以便减少或预防多于一种物种的葡萄球菌细菌的严重感染。 以这种方式产生的抗体也已显示出显示置换活性，因此可有利地用于其中将这些抗体施用于具有预先存在的葡萄球菌感染的患者的方法，因为能够从细胞外基质上的结合位点置换细菌蛋白质 。 最后，还提供了鉴定，分离和利用置换抗体的方法。

公开(公告)号：US06288214B1

公开(公告)日：2001-09-11

申请号：US08856253

申请日：1997-05-14

申请人： Magnus Höök ,  Joseph M. Patti ,  Karen House-Pompeo ,  Narayana Sthanam ,  Jindrich Symersky

发明人： Magnus Höök ,  Joseph M. Patti ,  Karen House-Pompeo ,  Narayana Sthanam ,  Jindrich Symersky

IPC分类号： C07K1600

CPC分类号： C07K14/31 ,  A61K38/00 ,  A61K39/00 ,  C07K16/1271 ,  C07K2317/77

摘要： Disclosed are the cna gene and cna-derived nucleic acid segments from Staphylococcus aureus, and DNA segments encoding cna from related bacteria. Also disclosed are Col binding protein (CBP) compositions and methods of use. The CBP protein and antigenic epitopes derived therefrom are contemplated for use in the treatment of pathological infections, and in particular, for use in the prevention of bacterial adhesion to Col. DNA segments encoding these proteins and anti-(Col binding protein) antibodies will also be of use in various screening, diagnostic and therapeutic applications including active and passive immunization and methods for the prevention of bacterial colonization in an animal such as a human. These DNA segments and the peptides derived therefrom are contemplated for use in the preparation of vaccines and, also, for use as carrier proteins in vaccine formulations, and in the formulation of compositions for use in the prevention of S. aureus infection.

摘要翻译： 公开了来自金黄色葡萄球菌的cna基因和cna衍生的核酸片段以及编码来自相关细菌的cna的DNA片段。 还公开了Col结合蛋白（CBP）组合物和使用方法。 CBP蛋白和源自其的抗原表位被考虑用于治疗病理感染，特别是用于预防细菌粘附到Col。编码这些蛋白质的DNA片段和抗（Col结合蛋白）抗体也将 可用于各种筛选，诊断和治疗应用，包括主动和被动免疫以及用于预防动物如人的细菌定植的方法。 这些DNA片段及其衍生的肽被考虑用于疫苗的制备，也用作疫苗制剂中的载体蛋白，以及用于预防金黄色葡萄球菌感染的组合物的配方。

公开(公告)号：US20090202578A1

公开(公告)日：2009-08-13

申请号：US12365481

申请日：2009-02-04

申请人： Timothy FOSTER ,  Fiona Roche ,  Mark Pallen ,  Joseph M. Patti ,  Jeff T. Hutchins ,  Pietro Speziale

发明人： Timothy FOSTER ,  Fiona Roche ,  Mark Pallen ,  Joseph M. Patti ,  Jeff T. Hutchins ,  Pietro Speziale

IPC分类号： A61K39/085 ,  A61K38/16 ,  G01N33/569

CPC分类号： C07K16/1271

摘要： Surface proteins are provided which generate polyclonal and monoclonal antibodies which are cross-reactive to both coagulase-positive staphylococcus bacteria, such as S. aureus and to coagulase-negative bacteria, such as S. epidermidis and S. hemolyticus. The antibodies may be generated from surface proteins that have been isolated on the basis of characteristics that may be common between S. aureus and coagulase-negative staphylococci, or the A domains of those surface proteins, and these recombinant surface proteins are used to generate the cross-reactive antibodies. Vaccines comprising an immunologically effective amount of the proteins are also provided, and these vaccines are used in methods for the treatment or protection against a wide variety of staphylococcal infections.

摘要翻译： 提供了表达蛋白，其产生与凝固酶阳性葡萄球菌如金黄色葡萄球菌和凝血酶阴性细菌如表皮葡萄球菌和溶血性葡萄球菌的交叉反应性的多克隆和单克隆抗体。 抗体可以从基于在金黄色葡萄球菌和凝固酶阴性葡萄球菌或这些表面蛋白质的A结构域可能是常见的特征分离的表面蛋白产生，并且这些重组表面蛋白质用于产生 交叉反应性抗体。 还提供了包含免疫有效量的蛋白质的疫苗，并且这些疫苗用于治疗或保护免受各种葡萄球菌感染的方法中。

公开(公告)号：US07855272B2

公开(公告)日：2010-12-21

申请号：US12546322

申请日：2009-08-24

申请人： Joseph M. Patti ,  Timothy J. Foster ,  Elisabet Josefsson ,  Deidre Ni Eidhin ,  Magnus A. O. Hook ,  Samuel E. Perkins

发明人： Joseph M. Patti ,  Timothy J. Foster ,  Elisabet Josefsson ,  Deidre Ni Eidhin ,  Magnus A. O. Hook ,  Samuel E. Perkins

IPC分类号： C07K1/00

CPC分类号： G01N33/56938 ,  A61K38/00 ,  A61K39/00 ,  C07K14/31 ,  G01N2469/00 ,  Y10S435/975 ,  Y10S530/81 ,  Y10S530/825

摘要： An isolated extracellular matrix-binding protein, designated as SdrE and its corresponding amino acid and nucleic acid sequences and motifs are described. The proteins, peptides, fragments thereof or antigenic portions thereof are useful for the prevention, inhibition, treatment and diagnosis of S. aureus infection and as scientific research tools. Further, antibodies or antibody fragments to the proteins, peptides, fragments thereof or antigenic portions thereof are also useful for the prevention, inhibition, treatment and diagnosis of S. aureus infection. In particular, the proteins or antibodies thereof may be administered to wounds or used to coat biomaterials to act as blocking agents to prevent or inhibit the binding of S. aureus to wounds or biomaterials.

摘要翻译： 描述了分离的细胞外基质结合蛋白，命名为SdrE及其相应的氨基酸和核酸序列和基序。 蛋白质，肽，其片段或其抗原部分可用于预防，抑制，治疗和诊断金黄色葡萄球菌感染以及科学研究工具。 此外，蛋白质，肽，其片段或其抗原部分的抗体或抗体片段也可用于预防，抑制，治疗和诊断金黄色葡萄球菌感染。 特别地，其蛋白质或其抗体可以施用于伤口或用于涂覆生物材料以用作阻断剂以预防或抑制金黄色葡萄球菌与伤口或生物材料的结合。

公开(公告)号：US20100081616A1

公开(公告)日：2010-04-01

申请号：US12546289

申请日：2009-08-24

申请人： Joseph M. Patti ,  Timothy J. Foster ,  Elisabet Josefsson ,  Deidre Ni Eidhin ,  Magnus A.O. Hook ,  Samuel E. Perkins

发明人： Joseph M. Patti ,  Timothy J. Foster ,  Elisabet Josefsson ,  Deidre Ni Eidhin ,  Magnus A.O. Hook ,  Samuel E. Perkins

IPC分类号： A61K38/16 ,  C07K14/31 ,  G01N33/569

CPC分类号： G01N33/56938 ,  A61K38/00 ,  A61K39/00 ,  C07K14/31 ,  G01N2469/00 ,  Y10S435/975 ,  Y10S530/81 ,  Y10S530/825

摘要： An isolated extracellular matrix-binding protein, designated as SdrD and its corresponding amino acid and nucleic acid sequences and motifs are described. The proteins, peptides, fragments thereof or antigenic portions thereof are useful for the prevention, inhibition, treatment and diagnosis of S. aureus infection and as scientific research tools. Further, antibodies or antibody fragments to the proteins, peptides, fragments thereof or antigenic portions thereof are also useful for the prevention, inhibition, treatment and diagnosis of S. aureus infection. In particular, the proteins or antibodies thereof may be administered to wounds or used to coat biomaterials to act as blocking agents to prevent or inhibit the binding of S. aureus to wounds or biomaterials.

摘要翻译： 描述了分离的细胞外基质结合蛋白，称为SdrD及其相应的氨基酸和核酸序列和基序。 蛋白质，肽，其片段或其抗原部分可用于预防，抑制，治疗和诊断金黄色葡萄球菌感染以及科学研究工具。 此外，蛋白质，肽，其片段或其抗原部分的抗体或抗体片段也可用于预防，抑制，治疗和诊断金黄色葡萄球菌感染。 特别地，其蛋白质或其抗体可以施用于伤口或用于涂覆生物材料以用作阻断剂以预防或抑制金黄色葡萄球菌与伤口或生物材料的结合。