公开(公告)号：US5783428A

公开(公告)日：1998-07-21

申请号：US804144

申请日：1997-02-20

申请人： Makoto Goto ,  Terukazu Nara ,  Izuru Tokumaru ,  Nobutake Fugono ,  Yasukazu Uchida ,  Masato Terasawa ,  Hideaki Yukawa

发明人： Makoto Goto ,  Terukazu Nara ,  Izuru Tokumaru ,  Nobutake Fugono ,  Yasukazu Uchida ,  Masato Terasawa ,  Hideaki Yukawa

IPC分类号： C12P7/46 ,  C12P13/20

CPC分类号： C12P7/46 ,  C12P13/20 ,  Y10S435/832 ,  Y10S435/833

摘要： Fumaric acid is produced by reacting a culture of a microorganism which produces maleate isomerase that exhibits a maximum activity at not less than 50.degree. C. or a treated product thereof with maleic acid in an aqueous solution, and isomerizing maleic acid to produce fumaric acid. L-aspartic acid is produced by reacting both of a culture of a microorganism which produces maleate isomerase or a treated product thereof and a culture of a microorganism which produces aspartase or a treated product thereof with maleic acid and ammonia in an aqueous solution, producing L-aspartic acid from maleic acid and ammonia by enzyme reactions, and recovering L-aspartic acid from the reaction mixture.

摘要翻译： 富马酸是通过使生成马来酸异构酶的微生物培养物（其在50℃以上表现出最大活性）或其处理产物与马来酸在水溶液中反应，并使马来酸异构化以产生富马酸而制备的。 L-天冬氨酸通过使水解产生马来酸异构酶的微生物培养物或其处理产物和产生天冬氨酰胺的微生物培养物或其处理产物与马来酸和氨反应，生成L - 天冬氨酸通过酶反应从马来酸和氨中回收，并从反应混合物中回收L-天冬氨酸。

公开(公告)号：US5149651A

公开(公告)日：1992-09-22

申请号：US691880

申请日：1991-04-26

申请人： Makoto Goto ,  Terukazu Nara ,  Yasukazu Uchida ,  Masato Terasawa ,  Hideaki Yukawa ,  Hisashi Yamagata

发明人： Makoto Goto ,  Terukazu Nara ,  Yasukazu Uchida ,  Masato Terasawa ,  Hideaki Yukawa ,  Hisashi Yamagata

IPC分类号： C12N1/20 ,  C12N9/88 ,  C12P13/06 ,  C12R1/38

CPC分类号： C12P13/06 ,  Y10S435/874 ,  Y10S435/876 ,  Y10S435/877

摘要： Microorganisms of the genus Pseudomonas containing aspartate beta-decarboxylase at a high level are produced by culturing said microorganism in a medium supplemented with a chelating agent. L-Alanine can be efficiently produced from L-aspartic acid said microorganisms or the treated product thereof.