摘要:
Microorganisms of the genus Pseudomonas containing aspartate beta-decarboxylase at a high level are produced by culturing said microorganism in a medium supplemented with a chelating agent. L-Alanine can be efficiently produced from L-aspartic acid said microorganisms or the treated product thereof.
摘要:
A process for producing an NADH oxidase which comprises culturing alkalophilic, facultative anaerobic NADH oxidase-producing bacteria having the optimum growth pH in an alkaline region in medium and collecting said NADH oxidase from the culture; and a plasmid used for the said process.
摘要:
A process for producing an NADH oxidase which comprises culturing alkalophilic, facultative anaerobic NADH oxidase-producing bacteria having the optimum growth pH in an alkaline region in medium and collecting said NADH oxidase from the culture; and a plasmid used for the said process.
摘要:
A process for cultivating a microorganism transformed with a recombinant plasmid at least containing (a) a DNA fragment containing a promoter and a regulator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment containing a desired structural gene which can be expressed by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3%, and thereby allowing the desired structural gene to be expressed in the microorganism.
摘要:
An aqueous solution containing fumaric acid is reacted in the presence of an aerobically cultured product of a microorganism belonging to the Brevibacterium genus and having resistance to .alpha.-aminobutyric acid, whereby L-malic acid is produced.