公开(公告)号：US06251672B1

公开(公告)日：2001-06-26

申请号：US09197470

申请日：1998-11-23

申请人： Manfred Kubbies ,  Peter Dörmer ,  Petra Meissner

发明人： Manfred Kubbies ,  Peter Dörmer ,  Petra Meissner

IPC分类号： C12N500

CPC分类号： C12N5/0068 ,  C12N2533/50

摘要： Mammalian cells which require contact with cell surface proteins for activation, differentiation and/or proliferation are cultured in contact with an immobilized mammalian cell, with an immobilized membrane vesicle from a mammalian cell or with immobilized membrane vesicles from at least two mammalian cell populations. The mammalian cell or membrane vesicle can be modified with a first partner of a biological binding pair, and immobilized on a solid carrier via a second partner of the biological binding pair. Culturing can be with a first immobilized membrane vesicle and subsequently with a second immobilized membrane vesicle. The membrane vesicle contains part of the natural surface of a mammalian cell that includes a signal triggering surface protein. The membrane vesicle can be immobilized via a binding partner of a cell surface molecule.

摘要翻译： 需要与细胞表面蛋白质接触以激活，分化和/或增殖的哺乳动物细胞与来自哺乳动物细胞的固定的膜囊泡或来自至少两个哺乳动物细胞群体的固定的膜囊泡接触固定的哺乳动物细胞培养。 哺乳动物细胞或膜囊泡可以用生物结合对的第一个配偶体修饰，并通过生物结合对的第二个配偶体固定在固体载体上。 可以用第一固定化膜囊泡培养，随后用第二固定化膜囊泡培养。 膜囊泡包含哺乳动物细胞的天然表面的一部分，其包括触发表面蛋白的信号。 膜泡可以通过细胞表面分子的结合配偶体固定。

公开(公告)号：US5152437A

公开(公告)日：1992-10-06

申请号：US662267

申请日：1991-02-28

申请人： Heinz-Dieter Klein ,  Petra Meissner ,  Norbert Ochs ,  Martin Schottler ,  Wolfgang Sievert

发明人： Heinz-Dieter Klein ,  Petra Meissner ,  Norbert Ochs ,  Martin Schottler ,  Wolfgang Sievert

IPC分类号： B67C9/00 ,  B67D7/02 ,  B67D7/34

CPC分类号： B67D7/0272 ,  B67D7/0283 ,  B67D7/0288 ,  B67D7/0294 ,  B67D7/344

摘要： In a device for connecting apparatus for withdrawing ultrapure liquids from containers, the connection plate 4 is provided with three bores 5, 6, 7, of which two bores 5, 6 each extend through a socket 8 for vertical pipes. The top plate 9 has an integrated valve body 23 for accommodating at least two valve heads 2, 3 and three bores 10, 11, 12, which are congruent with the bores 5, 6, 7 of the connection plate 4. The bore 12 extends through the valve body 23, and the two other bores 10, 11 terminate, at one end thereof, in pipe sockets 29, 30, which project into the bores 5, 6 of the connection plate 4, and, at the other end thereof, in channels 31, 32, 33, 34 for the ultrapure liquids, which are arranged in the valve body 23 and are interrupted by the valve heads 2, 3.

摘要翻译： 在用于从容器中取出超纯液体的连接装置的连接装置中，连接板4设置有三个孔5,6,7，其中两个孔5,6各自延伸穿过用于垂直管的插座8。 顶板9具有集成的阀体23，用于容纳与连接板4的孔5,6,7相一致的至少两个阀头2,3和三个孔10,11,12。孔12延伸 通过阀体23，另外两个孔10,11在其一端终止于插入连接板4的孔5,6中的管座29,30中，而在其另一端， 在用于超纯液体的通道31,32,33,34中，其布置在阀体23中并被阀头2,3中断。

公开(公告)号：US09428727B2

公开(公告)日：2016-08-30

申请号：US13643998

申请日：2011-04-25

申请人： Christian Leist ,  Petra Meissner ,  Jörg Schmidt

发明人： Christian Leist ,  Petra Meissner ,  Jörg Schmidt

IPC分类号： C12N5/02 ,  C12N5/00 ,  C12N5/07 ,  C12N5/071 ,  C12N5/10 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N5/005 ,  C12N5/0018 ,  C12N2500/05 ,  C12N2500/12

摘要： The present application describes an optimized medium for growth of mammalian cells as well as polypeptide production. The cell culture medium is characterized by a Sow ratio of sodium to potassium ions, it further relates to the method of producing polypeptides using such cell culture media. Sn another aspect, the method of polypeptide production can also comprise a temperature shift and/or a pH-shift to further optimize growth and product yield.

摘要翻译： 本申请描述了用于哺乳动物细胞生长和多肽生产的优化培养基。 细胞培养基的特征在于钠离子与钾离子的母体比例，其还涉及使用这种细胞培养基产生多肽的方法。 另一方面，多肽生产的方法还可以包括温度变化和/或pH转换，以进一步优化生长和产物产率。

公开(公告)号：US20130122543A1

公开(公告)日：2013-05-16

申请号：US13643998

申请日：2011-04-25

申请人： Christian Leist ,  Petra Meissner ,  Jörg Schmidt

发明人： Christian Leist ,  Petra Meissner ,  Jörg Schmidt

IPC分类号： C12N5/00

CPC分类号： C12N5/005 ,  C12N5/0018 ,  C12N2500/05 ,  C12N2500/12

摘要： The present application describes an optimized medium for growth of mammalian cells as well as polypeptide production. The cell culture medium is characterized by a Sow ratio of sodium to potassium ions, it further relates to the method of producing polypeptides using such cell culture media. Sn another aspect, the method of polypeptide production can also comprise a temperature shift and/or a pH-shift to further optimize growth and product yield.

摘要翻译： 本申请描述了用于哺乳动物细胞生长和多肽生产的优化培养基。 细胞培养基的特征在于钠离子与钾离子的母体比例，其还涉及使用这种细胞培养基产生多肽的方法。 另一方面，多肽生产的方法还可以包括温度变化和/或pH转换，以进一步优化生长和产物产率。