摘要:
A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译:提供了在植物或植物的一部分内合成流感病毒样颗粒(VLP)的方法。 该方法包括在植物中表达A / California / 04/09型流感病毒HA,并通过大小排阻色谱法纯化。 本发明还涉及包含A / California / 04/09型流感HA蛋白和植物脂质的VLP。 本发明还涉及编码A / California / 04/09型流感病毒的核酸以及载体。 VLP可用于制定流感疫苗,或可用于丰富现有疫苗。
摘要:
A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译:提供了在植物或植物的一部分内合成流感病毒样颗粒(VLP)的方法。 该方法包括在植物中表达A / California / 04/09型流感病毒HA,并通过大小排阻色谱法纯化。 本发明还涉及包含A / California / 04/09型流感HA蛋白和植物脂质的VLP。 本发明还涉及编码A / California / 04/09型流感病毒的核酸以及载体。 VLP可用于制定流感疫苗,或可用于丰富现有疫苗。
摘要:
The present invention relates to a method for enhancing the yield of recombinant protein produced in genetically transformed plants. The invention most particularly relates to a method for preventing the undesirable proteolysis of recombinant proteins after harvest of the plant, during processing of the products from the plants. Especially, this invention focuses on introducing protease inhibitors in plants to prevent undesirable proteolysis of recombinant proteins at the time of cell disruption during the extraction process.
摘要:
The present invention relates to a method for increasing the stability of endogenous proteins recovered from plant cells or plants. The preservation of endogenous proteins integrity of endogenous protein occurs by neutralizing proteolysis in crude extracts, particularly by the use of genetic alteration of plant cells or plants that express recombinant protease inhibitors or altered activity of specific target proteases.