Method for enzymatic preparation of homogentisate
    3.
    发明授权
    Method for enzymatic preparation of homogentisate 有权
    方酸酶的制备方法

    公开(公告)号:US06812010B1

    公开(公告)日:2004-11-02

    申请号:US09582482

    申请日:2000-10-13

    IPC分类号: C12P740

    CPC分类号: C12P7/42

    摘要: The invention concerns a method for enzymatic preparation of homogentisate (HMO) from 4-hydroxypyruvate (HPP), characterized in that it consists in carrying out in a suitable reaction medium the following enzymatic reactions: enzymatic conversion of HPP into 4-hydroxyphenylacetate (HPA) with a first appropriate enzyme; then an enzymatic conversion of HPA into HMO with a second appropriate enzyme.

    摘要翻译: 本发明涉及从4-羟基丙酮酸(HPP)酶促制备果糖酸盐(HMO)的方法,其特征在于其在合适的反应介质中进行以下酶促反应:将HPP酶促转化为4-羟基苯基乙酸酯(HPA) 用第一适当的酶; 然后用第二种合适的酶将HPA酶促转化成HMO。

    Chimeric gene for the transformation of plants
    4.
    发明授权
    Chimeric gene for the transformation of plants 失效
    用于植物转化的嵌合基因

    公开(公告)号:US5510471A

    公开(公告)日:1996-04-23

    申请号:US251621

    申请日:1994-05-31

    摘要: Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyadenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localised enzyme, a partial sequence of the N-terminal mature part of a plant gene encoding a plastid-localised enzyme and then a second transit peptide of a plant gene encoding a plastid- localised enzyme. Production of glyphosate-tolerant plants is disclosed.

    摘要翻译: 用于赋予植物对具有作为其目标EPSPS的除草剂的增加的耐受性的嵌合基因包括在转录方向上的启动子区,转运肽区,草甘膦耐受的编码序列和多聚腺苷酸化信号区,其中转运肽 翻译方向包括编码质体定位酶的植物基因的至少一个转运肽,编码质体定位酶的植物基因的N末端成熟部分的部分序列,然后是第二转运 编码质体定位酶的植物基因的肽。 公开了耐草甘膦植物的生产。

    Expression cassette encoding a 5-enolpyruvylshikimate-3-phosphate synthase (epsps) and herbicde-tolerant plants containing it
    8.
    发明申请
    Expression cassette encoding a 5-enolpyruvylshikimate-3-phosphate synthase (epsps) and herbicde-tolerant plants containing it 审中-公开
    编码5-烯醇丙酮酸莽草酸-3-磷酸合成酶(epsps)和含有其的耐受草药植物的表达盒

    公开(公告)号:US20060242727A1

    公开(公告)日:2006-10-26

    申请号:US10538438

    申请日:2003-12-10

    IPC分类号: A01H1/00 C12N15/82 C12N5/04

    CPC分类号: C12N15/8275

    摘要: The present invention relates to a novel expression cassette comprising a nucleic acid sequence encoding an EPSPS. In particular, the present invention relates to a novel expression cassette comprising, in the direction of transcription, functionally linked to one another, a promoter regulatory sequence which is functional in plant cells or plants, a nucleic acid sequence encoding an EPSPS and a terminator sequence which is functional in plant cells or plants, characterized in that the promoter regulatory sequence is a nucleic acid sequence chosen from the promoter regulatory sequences of the CsVMV (Cassaya Vein Mosaic Virus) plant virus.

    摘要翻译: 本发明涉及包含编码EPSPS的核酸序列的新型表达盒。 特别地,本发明涉及一种新的表达盒,其在转录方向上包括在功能上彼此相连的启动子调节序列,其在植物细胞或植物中是有功能的,编码EPSPS和终止子序列的核酸序列 其在植物细胞或植物中是功能性的,其特征在于启动子调节序列是选自CsVMV(Cassaya Vein Mosaic Virus)植物病毒的启动子调控序列的核酸序列。