-
公开(公告)号:US20120070860A1
公开(公告)日:2012-03-22
申请号:US13375153
申请日:2010-06-02
申请人: Marco Jenzsch , Max Lechner , Michael Pohlscheidt , Christoph Reese , Alexander Scholz , Hermann Tebbe
发明人: Marco Jenzsch , Max Lechner , Michael Pohlscheidt , Christoph Reese , Alexander Scholz , Hermann Tebbe
CPC分类号: B01F7/00641 , B01F7/0025 , B01F7/00266 , B01F7/00341 , C12M27/02
摘要: The present patent application describes a stirrer comprising a combination of at least one axially-conveying element and at least one radially-conveying element relative to the rotary shaft of the stirrer wherein the largest diameter of the at least one axially-conveying element is equal to or less than the inner diameter di of the radially-conveying element. In one embodiment the stirrer according to the invention is a combination of one anchor stirrer with at least one inclined-blade stirrer. Furthermore the use of the stirrer according to the invention for the culture of cells in a dialysis method is described.
摘要翻译: 本专利申请描述了一种搅拌器,其包括相对于搅拌器的旋转轴的至少一个轴向传送元件和至少一个径向传送元件的组合,其中至少一个轴向传送元件的最大直径等于 或小于径向输送元件的内径di。 在一个实施方案中,根据本发明的搅拌器是一个锚式搅拌器与至少一个倾斜翼式搅拌器的组合。 此外,描述了在透析法中使用根据本发明的搅拌器进行细胞培养。
-
公开(公告)号:US10076731B2
公开(公告)日:2018-09-18
申请号:US13375153
申请日:2010-06-02
申请人: Marco Jenzsch , Max Lechner , Michael Pohlscheidt , Christoph Reese , Alexander Scholz , Hermann Tebbe
发明人: Marco Jenzsch , Max Lechner , Michael Pohlscheidt , Christoph Reese , Alexander Scholz , Hermann Tebbe
CPC分类号: B01F7/00641 , B01F7/0025 , B01F7/00266 , B01F7/00341 , C12M27/02
摘要: The present patent application describes a stirrer comprising a combination of at least one axially-conveying element and at least one radially-conveying element relative to the rotary shaft of the stirrer wherein the largest diameter of the at least one axially-conveying element is equal to or less than the inner diameter di of the radially-conveying element. In one embodiment the stirrer according to the invention is a combination of one anchor stirrer with at least one inclined-blade stirrer. Furthermore the use of the stirrer according to the invention for the culture of cells in a dialysis method is described.
-
公开(公告)号:US20120135464A1
公开(公告)日:2012-05-31
申请号:US13386302
申请日:2010-07-23
CPC分类号: C12M27/02 , B01F7/00241 , B01F7/00341 , B01F7/00383 , B01F7/00466 , B01F7/00641 , B01F7/1625 , B01F2215/0073 , C07K16/2863 , C07K16/2887 , C07K16/40 , C07K2317/14 , C12M27/08 , C12N5/0602
摘要: The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures.
摘要翻译: 本发明涉及用于动物细胞培养的搅拌器系统,其由至少一个径向输送的搅拌器元件和至少一个轴向输送的搅拌器组件的组合组成,其中必须存在至少三个搅拌器元件,最上面的搅拌器元件是 轴向输送搅拌器元件。 搅拌器元件在搅拌器轴上相互一定距离地布置。 一个具体的实施方案是多个搅拌器系统,其由作为径向输送搅拌器元件的两个盘式搅拌器和作为轴向输送搅拌器元件的倾斜叶片搅拌器组成,其中倾斜叶片搅拌器设置在搅拌器轴上的盘式搅拌器上方。 根据本发明的搅拌器系统尤其实现了在细胞培养物中对剪切敏感的哺乳动物细胞的培养中更温和和更好的混合。
-
公开(公告)号:US07666654B2
公开(公告)日:2010-02-23
申请号:US11784399
申请日:2007-04-05
申请人: Michael Pohlscheidt , Berthold Boedeker , Torsten Minuth , Heiner Apeler , Uwe Langer , Katrin Brabender , Dirk Otto-Brabender , Joachim Kerper , Hans-Juergen Henzler
发明人: Michael Pohlscheidt , Berthold Boedeker , Torsten Minuth , Heiner Apeler , Uwe Langer , Katrin Brabender , Dirk Otto-Brabender , Joachim Kerper , Hans-Juergen Henzler
CPC分类号: C12N7/00 , C12N2531/00 , C12N2710/00051
摘要: The invention relates to a method for preparing viral suspensions. The invention relates in particular to a method for preparing high-titer viral suspensions in cell cultures. Preferred methods include increasing the volume of the cell culture prior to infection with viral material and subsequent further steps of expanding the volume to a final volume which is distinctly larger than the maximum culture volume prior to infection.
摘要翻译: 本发明涉及一种制备病毒性悬浮液的方法。 本发明特别涉及在细胞培养物中制备高滴度病毒悬浮液的方法。 优选的方法包括在用病毒材料感染之前增加细胞培养物的体积,随后进一步将体积扩大至明显大于感染前的最大培养体积的最终体积。
-
公开(公告)号:US20080014626A1
公开(公告)日:2008-01-17
申请号:US11784399
申请日:2007-04-05
申请人: Michael Pohlscheidt , Berthold Boedeker , Torsten Minuth , Heiner Apeler , Uwe Langer , Katrin Brabender , Dirk Otto-Brabender , Joachim Kerper , Hans-Juergen Henzler
发明人: Michael Pohlscheidt , Berthold Boedeker , Torsten Minuth , Heiner Apeler , Uwe Langer , Katrin Brabender , Dirk Otto-Brabender , Joachim Kerper , Hans-Juergen Henzler
IPC分类号: C12N7/02
CPC分类号: C12N7/00 , C12N2531/00 , C12N2710/00051
摘要: The invention relates to a method for preparing viral suspensions. The invention relates in particular to a method for preparing high-titer viral suspensions in cell cultures. Preferred methods include increasing the volume of the cell culture prior to infection with viral material and subsequent further steps of expanding the volume to a final volume which is distinctly larger than the maximum culture volume prior to infection.
摘要翻译: 本发明涉及一种制备病毒性悬浮液的方法。 本发明特别涉及在细胞培养物中制备高滴度病毒悬浮液的方法。 优选的方法包括在用病毒材料感染之前增加细胞培养物的体积,随后进一步将体积扩大至明显大于感染前的最大培养体积的最终体积。
-
-
-
-