公开(公告)号：US5665564A

公开(公告)日：1997-09-09

申请号：US103319

申请日：1993-08-09

申请人： Marinella Caruso ,  Anna Luisa Colombo ,  Luisa Garofano ,  Francesca Torti ,  Guiseppe Zanella

发明人： Marinella Caruso ,  Anna Luisa Colombo ,  Luisa Garofano ,  Francesca Torti ,  Guiseppe Zanella

IPC分类号： C07K14/36 ,  C12N15/65 ,  C12P19/56 ,  C12N5/00 ,  C07H17/00 ,  C12N15/00

CPC分类号： C07K14/36 ,  C12N15/65 ,  C12P19/56

摘要： Doxorubicin resistance can be conferred on a host by transforming the host with a recombinant vector comprising a DNA having the configuration of restriction sites shown in FIGS. 1 or 2 of the accompanying drawings or a restriction fragment derived therefrom containing a gene coding for doxorubicin resistance.

摘要翻译： 通过用包含具有如图1和2所示的限制性位点构型的DNA的重组载体转化宿主，可以在宿主上赋予多柔比星抗性。 附图中的1或2个或其衍生的限制性片段含有编码多柔比星抗性的基因。

公开(公告)号：US06528301B1

公开(公告)日：2003-03-04

申请号：US09622588

申请日：2000-08-21

申请人： Umberto Breme ,  Marinella Caruso ,  Nicoletta Crespi Perellino ,  Lorena Fedeli ,  Andrea Pavesi ,  Luca Piacenza ,  Giambattista Ventrella

发明人： Umberto Breme ,  Marinella Caruso ,  Nicoletta Crespi Perellino ,  Lorena Fedeli ,  Andrea Pavesi ,  Luca Piacenza ,  Giambattista Ventrella

IPC分类号： C12N120

CPC分类号： C12R1/01 ,  C12P17/02

摘要： The present invention related to microorganisms producing taxanes, such as paclitaxel or related taxanes, to the use of said microorganisms for the production of taxanes, and to the procedure for the isolation of said microorganisms from plants. In particular, the present invention relates to microorganisms of the Actinomycetes group producing taxanes as well as to the biologically pure cultures of a strain of the Actinomycetes group which produces taxanes. The microorganisms of Actinomycetes group are preferably belonging to the suprageneric groups of Streptomycetes, Actinoplanetes, Maduromycetes, Thermomonosporas or Nocardioforms, more preferably they are belonging to genus Streptomyces, Actinoplanes, Nocardiopsis, Micromonospora, Actinomadura or Kitasatosporia, still more preferably to the genus Kitasatosporia. A particularly preferred microorganism is Kitasatosporia sp. CECT 4991.

摘要翻译： 本发明涉及使用所述微生物生产紫杉烷类的微生物生产紫杉烷类的紫杉醇或相关紫杉烷类的微生物以及从植物中分离所述微生物的方法。 特别地，本发明涉及产生紫杉烷类的放线菌属组的微生物以及产生紫杉烷类的放线菌属组的生物纯培养物。 放线菌属组的微生物优选属于链霉菌属，放线菌纲，马齿苋属，热单孢菌属或诺卡氏菌属的超重组，更优选属于链霉菌属，动植物型，诺卡氏菌属，小单孢菌属，放线菌属或小孢子虫属，还更优选属于北沙氏菌属。 特别优选的微生物是Kitasatosporia sp。 CECT 4991。