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公开(公告)号：US07615621B2
公开(公告)日：2009-11-10
申请号：US11542046
申请日：2006-10-03
申请人： Mark A. Dizigan , Rebecca A. Kelly , Dale A. Voyles , Michael Hans Luethy , Thomas M. Malvar , Kathleen P. Malloy
发明人： Mark A. Dizigan , Rebecca A. Kelly , Dale A. Voyles , Michael Hans Luethy , Thomas M. Malvar , Kathleen P. Malloy
CPC分类号： C12N15/8254 , A01H5/10 , C12Q1/6895
摘要： Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.
摘要翻译： 本文公开了基于插入到玉米基因组中的外源DNA构建体的DNA序列和插入位点侧翼的基因组序列来检测赖氨酸增加的转基因事件的存在的测定。 还提供了具有表达二氢吡啶甲酸合酶的新型外源DNA构建体的转基因植物，其活性导致植物或植物产物中赖氨酸增加。
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公开(公告)号：US07157281B2
公开(公告)日：2007-01-02
申请号：US11004221
申请日：2004-12-02
申请人： Mark A. Dizigan , Rebecca A. Kelly , Dale A. Voyles , Michael Hans Luethy , Thomas M. Malvar , Kathleen P. Malloy
发明人： Mark A. Dizigan , Rebecca A. Kelly , Dale A. Voyles , Michael Hans Luethy , Thomas M. Malvar , Kathleen P. Malloy
CPC分类号： C12N15/8254 , A01H5/10 , C12Q1/6895
摘要： Disclosed herein are novel DNA constructs encoding dihydrodipicolinic acid synthase that are useful for generating transgenic events. Also provided are transgenic plants harboring such DNA constructs, the expression of which results in increased lysine in the plant or plant product. Also provided are maize plants having LY038 Event and progeny thereof having this event. Additionally disclosed are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site.
摘要翻译： 本文公开了编码二氢吡啶二羧酸合酶的新型DNA构建体，其可用于产生转基因事件。 还提供了含有这样的DNA构建体的转基因植物，其表达导致植物或植物产物中赖氨酸增加。 还提供了具有LY038事件的玉米植物，其后代具有此事件。 另外公开了基于插入到玉米基因组中的外源DNA构建体的DNA序列和插入位点侧翼的基因组序列检测赖氨酸增加的转基因事件的存在的测定法。
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公开(公告)号：US08236938B2
公开(公告)日：2012-08-07
申请号：US12590529
申请日：2009-11-09
申请人： Mark A. Dizigan , Rebecca A. Kelly , Dale A. Voyles , Michael Hans Luethy , Thomas M. Malvar , Kathleen P. Malloy
发明人： Mark A. Dizigan , Rebecca A. Kelly , Dale A. Voyles , Michael Hans Luethy , Thomas M. Malvar , Kathleen P. Malloy
CPC分类号： C12N15/8254 , A01H5/10 , C12Q1/6895
摘要： Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.
摘要翻译： 本文公开了基于插入到玉米基因组中的外源DNA构建体的DNA序列和插入位点侧翼的基因组序列来检测赖氨酸增加的转基因事件的存在的测定。 还提供了具有表达二氢吡啶甲酸合酶的新型外源DNA构建体的转基因植物，其活性导致植物或植物产物中赖氨酸增加。
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公开(公告)号：US20100212051A1
公开(公告)日：2010-08-19
申请号：US12590529
申请日：2009-11-09
申请人： Mark A. Dizigan , Rebecca A. Kelly , Dale A. Voyles , Michael Hans Luethy , Thomas M. Malvar , Kathleen P. Malloy
发明人： Mark A. Dizigan , Rebecca A. Kelly , Dale A. Voyles , Michael Hans Luethy , Thomas M. Malvar , Kathleen P. Malloy
CPC分类号： C12N15/8254 , A01H5/10 , C12Q1/6895
摘要： Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.
摘要翻译： 本文公开了基于插入到玉米基因组中的外源DNA构建体的DNA序列和插入位点侧翼的基因组序列来检测赖氨酸增加的转基因事件的存在的测定。 还提供了具有表达二氢吡啶甲酸合酶的新型外源DNA构建体的转基因植物，其活性导致植物或植物产物中赖氨酸增加。
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公开(公告)号：US20110113514A1
公开(公告)日：2011-05-12
申请号：US12909466
申请日：2010-10-21
IPC分类号： A01H5/10
CPC分类号： C12N15/8254
摘要： Disclosed herein are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase and a gene suppression molecule for lysine ketoglutarate reductase, the activity of which results in increased lysine in a plant seed.
摘要翻译： 本文公开了具有表达二氢吡啶二羧酸合酶的新型外源DNA构建体和赖氨酸酮戊二酸还原酶的基因抑制分子的转基因植物，其活性导致植物种子中赖氨酸增加。
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公开(公告)号：US07227056B2
公开(公告)日：2007-06-05
申请号：US10614076
申请日：2003-07-03
申请人： Leigh H. English , Susan M. Brussock , Thomas M. Malvar , James W. Bryson , Caroline A. Kulesza , Frederick S. Walters , Stephen L. Slatin , Michael A. Von Tersch , Charles Romano
发明人： Leigh H. English , Susan M. Brussock , Thomas M. Malvar , James W. Bryson , Caroline A. Kulesza , Frederick S. Walters , Stephen L. Slatin , Michael A. Von Tersch , Charles Romano
CPC分类号： C07K14/325 , Y10S977/925
摘要： Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis δ-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.
摘要翻译： 公开了包含编码鞘翅目昆虫毒素苏云金芽孢杆菌δ-内毒素的合成修饰基因的核酸片段。 还公开了使用这些基因重组表达多肽的方法，含有该基因的载体的制备以及用于转化合适的宿主细胞的方法。
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公开(公告)号：USRE39580E1
公开(公告)日：2007-04-17
申请号：US10061082
申请日：2002-01-31
申请人： Leigh H. English , Susan M. Brussock , Thomas M. Malvar , James W. Bryson , Caroline A. Kulesza , Frederick S. Walters , Stephen L. Slatin , Michael A. Von Tersch , Charles Romano
发明人： Leigh H. English , Susan M. Brussock , Thomas M. Malvar , James W. Bryson , Caroline A. Kulesza , Frederick S. Walters , Stephen L. Slatin , Michael A. Von Tersch , Charles Romano
CPC分类号： C07K14/325
摘要： The invention provides transgenic plants and transformed host cells which express modified cry 3B genes with enhanced toxicity to Coleopteran insects. Also disclosed are methods of making and using these transgenic plants, methods of making recombinant host cells expressing these δ-endotoxins, and methods of killing insects such as Colorado potato beetle (Leptinotarsa decemlineata), southern corn rootworm (Diabrotica undecimpunctata howardi Barber) and western corn rootworm (Diabrotica virgifera virgifera LeConte.
摘要翻译： 本发明提供转基因植物和转化的宿主细胞，其表达对鞘翅目昆虫具有增强的毒性的修饰的cry 3B基因。 还公开了制备和使用这些转基因植物的方法，制备表达这些δ-内毒素的重组宿主细胞的方法，以及杀死昆虫的方法，例如科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），南方玉米根虫（Diabrotica undecimpunctata howardi Barber）和西方 玉米根虫（Diabrotica virgifera virgifera LeConte。
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8.发明授权Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins 失效编码改良的苏云金芽孢杆菌鞘翅目毒性晶体蛋白的核酸片段
公开(公告)号：US6060594A
公开(公告)日：2000-05-09
申请号：US993722
申请日：1997-12-18
申请人： Leigh H. English , Susan M. Brussock , Thomas M. Malvar , James W. Bryson , Caroline A. Kulesza , Frederick S. Walters , Stephen L. Slatin , Michael A. Von Tersch , Charles Romano
发明人： Leigh H. English , Susan M. Brussock , Thomas M. Malvar , James W. Bryson , Caroline A. Kulesza , Frederick S. Walters , Stephen L. Slatin , Michael A. Von Tersch , Charles Romano
IPC分类号： C07K14/325 , C12N15/32 , C12N15/83 , C12N15/82
CPC分类号： C07K14/325 , Y10S977/925
摘要： Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis .delta.-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.
摘要翻译： 公开了包含编码鞘翅目有毒的苏云金芽孢杆菌δ-内毒素的合成修饰基因的核酸片段。 还公开了使用这些基因重组表达多肽的方法，含有该基因的载体的制备以及用于转化合适的宿主细胞的方法。
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公开(公告)号：US08344207B2
公开(公告)日：2013-01-01
申请号：US13114026
申请日：2011-05-23
申请人： Natalia N. Bogdanova , David R. Corbin , Thomas M. Malvar , Frederick J. Perlak , James K. Roberts , Charles P. Romano
发明人： Natalia N. Bogdanova , David R. Corbin , Thomas M. Malvar , Frederick J. Perlak , James K. Roberts , Charles P. Romano
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The present invention provides nucleotide sequences encoding an insecticidal protein exhibiting lepidopteran inhibitory activity, as well as a novel insecticidal protein referred to herein as a Cry1A.105 insecticide, transgenic plants expressing the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.
摘要翻译： 本发明提供编码表现鳞翅目昆虫抑制活性的杀虫蛋白的核苷酸序列，以及本文称为Cry1A.105杀虫剂的新型杀虫蛋白，表达杀虫剂的转基因植物，以及检测核苷酸序列或 生物样品中的杀虫剂。
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公开(公告)号：US20120185974A1
公开(公告)日：2012-07-19
申请号：US13436027
申请日：2012-03-30
申请人： David R. Corbin , Barry S. Goldman , Gregory J. Hinkle , Joseph E. Huesing , Thomas M. Malvar , Karina C. Krasomil-Osterfeld , Steven C. Slater , Sergei Spiridonov
发明人： David R. Corbin , Barry S. Goldman , Gregory J. Hinkle , Joseph E. Huesing , Thomas M. Malvar , Karina C. Krasomil-Osterfeld , Steven C. Slater , Sergei Spiridonov
CPC分类号： C07K14/24
摘要： The present invention relates to a nucleic acid sequence from Xenorhabdus that encodes a GroEL protein. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's and fragments of rRNA's. In addition, proteins and fragments of proteins so encoded and antibodies capable of binding the proteins are encompassed by the present invention. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, RNA's, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 本发明涉及来自Xenorhabdus的编码GroEL蛋白的核酸序列。 本发明包括非编码区中存在的核酸分子以及编码蛋白质，蛋白质片段，tRNA，tRNA片段，rRNA和rRNA片段的核酸分子。 此外，蛋白质和如此编码的蛋白质的片段以及能够结合蛋白质的抗体包括在本发明中。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段，RNA和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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