公开(公告)号：US08440880B2

公开(公告)日：2013-05-14

申请号：US13436027

申请日：2012-03-30

申请人： David R. Corbin ,  Barry S. Goldman ,  Gregory J. Hinkle ,  Joseph E. Huesing ,  Thomas M. Malvar ,  Karina C. Krasomil-Osterfeld ,  Steven C. Slater ,  Sergei Spiridonov

发明人： David R. Corbin ,  Barry S. Goldman ,  Gregory J. Hinkle ,  Joseph E. Huesing ,  Thomas M. Malvar ,  Karina C. Krasomil-Osterfeld ,  Steven C. Slater ,  Sergei Spiridonov

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N1/00 ,  C12N15/11 ,  C12N15/31 ,  C12N15/82

CPC分类号： C07K14/24

摘要： The present invention relates to a nucleic acid sequences from Xenorhabdus that encodes a GroEL protein. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's and fragments of rRNA's. In addition, protein and fragments of proteins so encoded and antibodies capable of binding the proteins are encompassed by the present invention. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, RNA'S, and antibodies, for example, for gene identification and analysis, and preparation of constructs.

摘要翻译： 本发明涉及来自Xenorhabdus的编码GroEL蛋白的核酸序列。 本发明包括非编码区中存在的核酸分子以及编码蛋白质，蛋白质片段，tRNA，tRNA片段，rRNA和rRNA片段的核酸分子。 此外，蛋白质和如此编码的蛋白质的片段以及能够结合蛋白质的抗体包括在本发明中。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段，RNA'S和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。

公开(公告)号：US08343764B2

公开(公告)日：2013-01-01

申请号：US12459621

申请日：2009-07-02

申请人： Mark Scott Abad ,  Marie A. Coffin ,  Barry S. Goldman

发明人： Mark Scott Abad ,  Marie A. Coffin ,  Barry S. Goldman

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10 ,  A01H1/00

CPC分类号： C12N15/8273 ,  C07K14/415 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  Y02A40/146

摘要： Transgenic seed for crops with improved traits are provided by trait-improving recombinant DNA in the nucleus of cells of the seed where plants grown from such transgenic seed exhibit one or more improved traits as compared to a control plant. The present invention provides recombinant DNA molecules for expression of a protein, including a recombinant DNA molecule for expression of a glutamine synthetase.

摘要翻译： 具有改善性状的作物的转基因种子通过在种子细胞核中改良重组DNA来提供，其中与对照植物相比，从这种转基因种子生长的植物表现出一种或多种改良的性状。 本发明提供用于表达蛋白质的重组DNA分子，包括用于表达谷氨酰胺合成酶的重组DNA分子。

公开(公告)号：US20110265221A1

公开(公告)日：2011-10-27

申请号：US12218251

申请日：2008-07-10

申请人： Mark Abad ,  Jeffrey E. Ahrens ,  Alice Clara Augustine ,  Erin Bell ,  Robert J. Bensen ,  Paolo Castiglioni ,  R. Eric Cerny ,  Shobha Char ,  Xianfeng Chen ,  Jaishree M. Chittoor-Vijayanath ,  Maureen Daley ,  Jill Deikman ,  Molian Deng ,  Todd Dezwaan ,  Stephen Duff ,  Michael D. Edgerton ,  Bradon J. Fabbri ,  Jason Fenner ,  Karen K. Gabbert ,  Meghan Galligan Donnarummo ,  Barry S. Goldman ,  Deborah J. Hawkins ,  Steve He ,  G. Richard Johnson ,  Balasulojini Karunanandaa ,  Garrett J. Lee ,  Paul Loida ,  Savitha Madappa ,  Robert J. Meister ,  Donald E. Nelson ,  Anand Pandravada ,  Obed Patty ,  Ming Peng ,  Marie Petracek ,  M.S. Rajani ,  Dhanalakshmi Ramachandra ,  Monica Ravanello ,  Thomas G. Ruff ,  Rick A. Sanders ,  Beth Savidge ,  Steven H. Schwartz ,  Padmini Sudarshana ,  Jindong Sun ,  Rebecca L. Thompson-Mize ,  Virginia Ursin ,  Dale L. Val ,  Srikanth Venkatachalayya ,  T.V. Venkatesh ,  K.R. Vidya ,  K. Vijayalakshmi ,  Todd C. Weber ,  Jingrui Wu ,  Zhidong Xie ,  Wei Zheng

发明人： Mark Abad ,  Jeffrey E. Ahrens ,  Alice Clara Augustine ,  Erin Bell ,  Robert J. Bensen ,  Paolo Castiglioni ,  R. Eric Cerny ,  Shobha Char ,  Xianfeng Chen ,  Jaishree M. Chittoor-Vijayanath ,  Maureen Daley ,  Jill Deikman ,  Molian Deng ,  Todd Dezwaan ,  Stephen Duff ,  Michael D. Edgerton ,  Bradon J. Fabbri ,  Jason Fenner ,  Karen K. Gabbert ,  Meghan Galligan Donnarummo ,  Barry S. Goldman ,  Deborah J. Hawkins ,  Steve He ,  G. Richard Johnson ,  Balasulojini Karunanandaa ,  Garrett J. Lee ,  Paul Loida ,  Savitha Madappa ,  Robert J. Meister ,  Donald E. Nelson ,  Anand Pandravada ,  Obed Patty ,  Ming Peng ,  Marie Petracek ,  M.S. Rajani ,  Dhanalakshmi Ramachandra ,  Monica Ravanello ,  Thomas G. Ruff ,  Rick A. Sanders ,  Beth Savidge ,  Steven H. Schwartz ,  Padmini Sudarshana ,  Jindong Sun ,  Rebecca L. Thompson-Mize ,  Virginia Ursin ,  Dale L. Val ,  Srikanth Venkatachalayya ,  T.V. Venkatesh ,  K.R. Vidya ,  K. Vijayalakshmi ,  Todd C. Weber ,  Jingrui Wu ,  Zhidong Xie ,  Wei Zheng

IPC分类号： A01H1/02 ,  C40B30/00 ,  A01H5/00 ,  A01H5/10 ,  C12N5/10 ,  C12N15/82

CPC分类号： C12N15/8273 ,  A01H1/02 ,  C12N15/8241 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8271 ,  C12Q1/6895 ,  C12Q2600/13 ,  Y02A40/146

摘要： This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

摘要翻译： 本发明提供重组DNA构建体，转基因植物核和具有这样的重组DNA构建体的细胞，用于表达可用于赋予转基因作物植物增强的农艺性状的蛋白质。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US07696340B2

公开(公告)日：2010-04-13

申请号：US11998426

申请日：2007-11-30

申请人： Barry S. Goldman ,  Karina Krasomil-Osterfeld ,  Thomas M. Malvar ,  John W. Pitkin ,  Steven C. Slater ,  Wei Wu ,  Jiamin Zeng

发明人： Barry S. Goldman ,  Karina Krasomil-Osterfeld ,  Thomas M. Malvar ,  John W. Pitkin ,  Steven C. Slater ,  Wei Wu ,  Jiamin Zeng

IPC分类号： C07H21/04 ,  C07K17/00 ,  A01H5/00

CPC分类号： C12N15/8286 ,  A01N63/00 ,  A01N63/02 ,  C07K14/24 ,  Y02A40/162

摘要： The invention provides isolated nucleotide sequences from Xenorhabdus nematophila species Xs86068, and, in particular, nucleotide sequences that encode insect inhibitory proteins, the insecticidal proteins, and compositions that comprise one or more of the insecticidal proteins for use in controlling insect infestation.

摘要翻译： 本发明提供了来自杀线虫（Xenorhabdus nematophila）Xs86068的分离的核苷酸序列，特别是编码昆虫抑制蛋白的核苷酸序列，杀虫蛋白以及包含一种或多种用于防治昆虫侵袭的杀虫蛋白的组合物。

公开(公告)号：US20090070898A1

公开(公告)日：2009-03-12

申请号：US11974469

申请日：2007-10-12

申请人： Edwards Allen ,  Barry S. Goldman ,  Liang Guo ,  Sara E. Heisel ,  Shihshieh Huang ,  Sergey I. Ivashuta ,  David K. Kovalik ,  Elysia K. Krieger ,  James K. Roberts ,  Yuanji Zhang

发明人： Edwards Allen ,  Barry S. Goldman ,  Liang Guo ,  Sara E. Heisel ,  Shihshieh Huang ,  Sergey I. Ivashuta ,  David K. Kovalik ,  Elysia K. Krieger ,  James K. Roberts ,  Yuanji Zhang

IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N5/00 ,  A01H5/00

CPC分类号： C12N15/113 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8238 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12N15/8286 ,  C12N2310/141 ,  C12N2320/50 ,  Y02A40/146 ,  Y02A40/162

摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.

摘要翻译： 本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。 包括显示营养反应表达的新型miRNA和miRNA前体。 还公开了miRNA诱饵序列。 还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。

公开(公告)号：US20090070897A1

公开(公告)日：2009-03-12

申请号：US11330903

申请日：2006-01-12

申请人： Barry S. Goldman ,  Bettina Darveaux ,  Jaclyn Cleveland ,  Mark Scott Abad ,  Mahmood Sayed

发明人： Barry S. Goldman ,  Bettina Darveaux ,  Jaclyn Cleveland ,  Mark Scott Abad ,  Mahmood Sayed

IPC分类号： C12N15/29 ,  A01H5/00 ,  C12N15/31 ,  A01G1/00

CPC分类号： C12N15/8273 ,  C07K14/415 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

摘要翻译： 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US20080295196A1

公开(公告)日：2008-11-27

申请号：US12001025

申请日：2007-12-06

申请人： Mark S. Abad ,  Barry S. Goldman ,  Jaishree M. Chittoor-Vijayanath ,  Marie Coffin ,  Ronald C. Rich

发明人： Mark S. Abad ,  Barry S. Goldman ,  Jaishree M. Chittoor-Vijayanath ,  Marie Coffin ,  Ronald C. Rich

IPC分类号： A01H1/04 ,  C12N5/10 ,  A01H5/00 ,  A01H1/02 ,  C12Q1/68

CPC分类号： C12N15/8271 ,  C07K14/415 ,  C12N15/8241 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8282 ,  C12N15/8283 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  G01N33/68 ,  G01N2333/415

摘要： Transgenic seed for crops with improved traits are provided by trait-improving recombinant DNA in the nucleus of cells of the seed where plants grown from such transgenic seed exhibit one or more improved traits as compared to a control plant. Of particular interest are transgenic plants that have increased yield. The present invention also provides recombinant DNA molecules for expression of a protein, and recombinant DNA molecules for suppression of a protein.

摘要翻译： 具有改善性状的作物的转基因种子通过在种子细胞核中改良重组DNA来提供，其中与对照植物相比，从这种转基因种子生长的植物表现出一种或多种改良的性状。 特别感兴趣的是具有增加的产量的转基因植物。 本发明还提供了用于表达蛋白质的重组DNA分子和用于抑制蛋白质的重组DNA分子。

公开(公告)号：US07319142B1

公开(公告)日：2008-01-15

申请号：US11216782

申请日：2005-08-31

申请人： Barry S. Goldman ,  Karina Krasomil-Osterfeld ,  Thomas M. Malvar ,  John W. Pitkin ,  Steven C. Slater ,  Wei Wu ,  Jiamin Zeng

发明人： Barry S. Goldman ,  Karina Krasomil-Osterfeld ,  Thomas M. Malvar ,  John W. Pitkin ,  Steven C. Slater ,  Wei Wu ,  Jiamin Zeng

IPC分类号： C07H21/04 ,  C07K14/00

CPC分类号： C12N15/8286 ,  A01N63/00 ,  A01N63/02 ,  C07K14/24 ,  Y02A40/162

摘要： The invention provides isolated nucleotide sequences from Xenorhabdus nematophila species Xs86068, and, in particular, nucleotide sequences that encode insect inhibitory proteins, the insecticidal proteins, and compositions that comprise one or more of the insecticidal proteins for use in controlling insect infestation.

摘要翻译： 本发明提供了来自杀线虫（Xenorhabdus nematophila）Xs86068的分离的核苷酸序列，特别是编码昆虫抑制蛋白的核苷酸序列，杀虫蛋白以及包含一种或多种用于防治昆虫侵袭的杀虫蛋白的组合物。

公开(公告)号：US09024113B2

公开(公告)日：2015-05-05

申请号：US11980183

申请日：2007-10-29

申请人： Yongwei Cao ,  Gregory J. Hinkle ,  Steven C. Slater ,  Xianfeng Chen ,  Barry S. Goldman

发明人： Yongwei Cao ,  Gregory J. Hinkle ,  Steven C. Slater ,  Xianfeng Chen ,  Barry S. Goldman

IPC分类号： C12N15/82 ,  A01H5/00 ,  C07K14/195

CPC分类号： C12N15/8247 ,  C07K14/195 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8279 ,  Y02A40/146

摘要： Recombinant constructs and methods useful for improvement of plants are provided. In particular, recombinant constructs comprising promoters functional in plant cells positioned for expression of polynucleotides encoding starch branching enzymes from microbial sources are provided. The disclosed constructs and methods find use in production of transgenic plants to provide plants, particularly crop plants, having improved yield.

摘要翻译： 提供了可用于改良植物的重组构建体和方法。 特别地，提供了包含在植物细胞中起作用的启动子的重组构建体，用于从微生物来源表达编码淀粉分支酶的多核苷酸。 所公开的构建体和方法可用于生产转基因植物以提供具有提高的产量的植物，特别是作物植物。

公开(公告)号：US08946511B2

公开(公告)日：2015-02-03

申请号：US11974469

申请日：2007-10-12

申请人： Edwards Allen ,  Barry S. Goldman ,  Liang Guo ,  Sara E. Heisel ,  Shihshieh Huang ,  Sergey I. Ivashuta ,  David K. Kovalik ,  Elysia K. Krieger ,  James K. Roberts ,  Yuanji Zhang

发明人： Edwards Allen ,  Barry S. Goldman ,  Liang Guo ,  Sara E. Heisel ,  Shihshieh Huang ,  Sergey I. Ivashuta ,  David K. Kovalik ,  Elysia K. Krieger ,  James K. Roberts ,  Yuanji Zhang

IPC分类号： C12N15/82 ,  C12N15/00 ,  A01H5/00

CPC分类号： C12N15/113 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8238 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12N15/8286 ,  C12N2310/141 ,  C12N2320/50 ,  Y02A40/146 ,  Y02A40/162

摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.