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公开(公告)号：US09006414B2
公开(公告)日：2015-04-14
申请号：US12973821
申请日：2010-12-20
IPC分类号： C12N15/113 , C12N15/82 , C07H21/02 , C12N9/06 , C12N15/11
CPC分类号： C12N15/113 , A61K48/00 , C07H21/02 , C12N9/0028 , C12N15/111 , C12N15/8218 , C12N15/8251 , C12N15/8254 , C12N2310/11 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物，通过从重组DNA构建体转录，其以5'至3'的顺序包含可操作地连接到反义取向DNA元件和互补DNA元件的启动子元件。
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公开(公告)号：US08461418B2
公开(公告)日：2013-06-11
申请号：US12277196
申请日：2008-11-24
CPC分类号： A01H5/10 , A23K10/30 , A23L7/198 , C07K14/415 , C12N9/0028 , C12N15/8218 , C12N15/8247 , C12N15/8251 , C12N15/8254
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物通过从包含5'至3'顺序的重组DNA构建体转录起可操作地连接到多于一个反义取向DNA元件和一个或多个互补DNA元件的启动子元件。
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公开(公告)号：US20110126321A1
公开(公告)日：2011-05-26
申请号：US12973821
申请日：2010-12-20
CPC分类号： C12N15/113 , A61K48/00 , C07H21/02 , C12N9/0028 , C12N15/111 , C12N15/8218 , C12N15/8251 , C12N15/8254 , C12N2310/11 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物，通过从重组DNA构建体转录，其以5'至3'的顺序包含可操作地连接到反义取向DNA元件和互补DNA元件的启动子元件。
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公开(公告)号：US20110113514A1
公开(公告)日：2011-05-12
申请号：US12909466
申请日：2010-10-21
IPC分类号： A01H5/10
CPC分类号： C12N15/8254
摘要： Disclosed herein are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase and a gene suppression molecule for lysine ketoglutarate reductase, the activity of which results in increased lysine in a plant seed.
摘要翻译： 本文公开了具有表达二氢吡啶二羧酸合酶的新型外源DNA构建体和赖氨酸酮戊二酸还原酶的基因抑制分子的转基因植物，其活性导致植物种子中赖氨酸增加。
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公开(公告)号：US07855323B2
公开(公告)日：2010-12-21
申请号：US11057062
申请日：2005-02-10
IPC分类号： C12N15/82
CPC分类号： C12N15/113 , A61K48/00 , C07H21/02 , C12N9/0028 , C12N15/111 , C12N15/8218 , C12N15/8251 , C12N15/8254 , C12N2310/11 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物，通过重组DNA构建体的转录，其以5'至3'的顺序包含可操作地连接到反义取向DNA元件和互补DNA元件的启动子元件。
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公开(公告)号：US20090158463A1
公开(公告)日：2009-06-18
申请号：US12277196
申请日：2008-11-24
CPC分类号： A01H5/10 , A23K10/30 , A23L7/198 , C07K14/415 , C12N9/0028 , C12N15/8218 , C12N15/8247 , C12N15/8251 , C12N15/8254
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物通过从包含5'至3'顺序的重组DNA构建体转录起可操作地连接到多于一个反义取向DNA元件和一个或多个互补DNA元件的启动子元件。
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公开(公告)号：US07683237B2
公开(公告)日：2010-03-23
申请号：US11077089
申请日：2005-03-10
申请人： Alan L. Kriz , Shihshieh Huang , Michael H. Luethy
发明人： Alan L. Kriz , Shihshieh Huang , Michael H. Luethy
CPC分类号： C12N15/8254
摘要： The present invention provides a transgenic maize plant having in its genome transgenic DNA including sequence for zein reduction and sequence for lysine biosynthesis, whereby expression of the transgenic DNA results in a synergistically increased lysine content of seed of the transgenic maize plant. The invention further provides a method for providing maize seed with synergistically increased lysine content.
摘要翻译： 本发明提供了一种转基因玉米植物，其基因组中含有玉米醇溶蛋白还原序列和赖氨酸生物合成序列的转基因DNA，由此转基因DNA的表达导致转基因玉米植物种子的赖氨酸含量的协同增加。 本发明还提供了一种为玉米种子提供协同增加的赖氨酸含量的方法。
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8.发明授权Engineered miRNA precursors for suppressing target gene expression in plants 有权用于抑制植物中靶基因表达的工程miRNA前体公开(公告)号：US07723568B2
公开(公告)日：2010-05-25
申请号：US11545106
申请日：2006-10-10
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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公开(公告)号：US20090100536A1
公开(公告)日：2009-04-16
申请号：US11879785
申请日：2007-07-18
申请人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
发明人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
CPC分类号： C12N15/8279 , A01D45/00 , A01N25/34 , A01N31/16 , A01N41/10 , A01N43/40 , A01N43/653 , A01N43/78 , A01N43/80 , A01N55/02 , C07K14/415 , C12N9/0071 , C12N15/8241 , C12N15/8243 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12Y114/12017 , A01N25/10 , A01N25/02 , A01N2300/00
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
摘要翻译： 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。
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10.发明申请公开(公告)号：US20100223694A1
公开(公告)日：2010-09-02
申请号：US12726673
申请日：2010-03-18
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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