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公开(公告)号：US6110696A
公开(公告)日：2000-08-29
申请号：US494668
申请日：1995-06-26
申请人： Mary E. Brown , Hans-Joachim Guder , John G. R. Hurrell , Lance S. Kuhn , Robert J. McEnroe , Rebecca W. Muddiman , Mary Luann Ochs
发明人： Mary E. Brown , Hans-Joachim Guder , John G. R. Hurrell , Lance S. Kuhn , Robert J. McEnroe , Rebecca W. Muddiman , Mary Luann Ochs
IPC分类号： C12Q1/00 , G01N33/535 , G01N33/542 , G01N33/543 , G01N33/58
CPC分类号： G01N33/581 , C12Q1/001 , G01N33/535 , G01N33/542 , G01N33/5438 , Y10S435/817 , Y10S435/968
摘要： A diagnostic kit, method, and apparatus for electrochemically determining the presence or concentration of an analyte in a sample. A mixture is formed which includes the sample, an enzyme acceptor polypeptide, an enzyme donor polypeptide, and a labeled substrate. The enzyme donor polypeptide is capable of combining with the enzyme acceptor polypeptide to form an active enzyme complex. The formation of such the active enzyme complex is responsive to the presence or concentration of the analyte in the fluid sample. The active enzyme hydrolyzes the labeled substrate, resulting in the generation of an electroactive label, which can then be oxidized at the surface of an electrode. A current resulting from the oxidation of the electroactive compound can be measured and correlated to the concentration of the analyte in the sample.
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公开(公告)号：US5427912A
公开(公告)日：1995-06-27
申请号：US113548
申请日：1993-08-27
IPC分类号： C12Q1/00 , G01N33/535 , G01N33/542 , G01N33/543 , G01N33/58
CPC分类号： G01N33/581 , C12Q1/001 , G01N33/535 , G01N33/542 , G01N33/5438 , Y10S435/817 , Y10S435/968
摘要： An immunoassay diagnostic kit, method, and apparatus for electrochemically determining the concentration of an analyte in a sample. A mixture is formed which includes the sample, an enzyme-acceptor polypeptide, an enzyme-donor polypeptide linked to an analyte analog (enzyme-donor polypeptide conjugate), a labeled substrate, and an antibody specific for the analyte to be measured. The analyte and the enzyme-donor polypeptide conjugate competitively bind to the antibody. When the enzyme-donor polypeptide conjugate is not bound to antibody, it will spontaneously combine with the enzyme acceptor polypeptide to form an active enzyme complex. The active enzyme hydrolyzes the labeled substrate, resulting in the generation of an electroactive label, which can then be oxidized at the surface of an electrode. A current resulting from the oxidation of the electroactive compound can be measured and correlated to the concentration of the analyte in the sample.
摘要翻译： 用于电化学测定样品中分析物浓度的免疫测定诊断试剂盒，方法和装置。 形成混合物，其包括样品，酶 - 受体多肽，与分析物类似物（酶供体多肽缀合物）连接的酶供体多肽，标记底物和对待测分析物特异性的抗体。 分析物和酶 - 供体多肽缀合物竞争性地结合抗体。 当酶供体多肽缀合物不与抗体结合时，它将自发地与酶受体多肽组合以形成活性酶复合物。 活性酶水解标记的底物，导致产生电活性标记，然后可以在电极表面被氧化。 可以测量由电活性化合物氧化产生的电流，并与样品中分析物的浓度相关。
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公开(公告)号：US5846702A
公开(公告)日：1998-12-08
申请号：US770975
申请日：1996-12-20
申请人： Zhi David Deng , Gerald F. Sigler , Nigel A. Surridge , Christopher D. Wilsey , Robert J. McEnroe , Walter W. Jernigan , Rebecca W. Muddiman
发明人： Zhi David Deng , Gerald F. Sigler , Nigel A. Surridge , Christopher D. Wilsey , Robert J. McEnroe , Walter W. Jernigan , Rebecca W. Muddiman
CPC分类号： C07F15/0026 , C12Q1/004 , Y10S435/817
摘要： A new group of Os(II) and Os(III) compounds useful as redox mediators in electrochemical biosensors. These compounds have 1) low oxidation potential, 2) fast reaction kinetics between the electroactive center of an enzyme and the compound, 3) slow oxidation of osmium by oxygen, and 4) excellent solubility in aqueous medium. These mediators are particularly useful as a component of a reagent used in an electrochemical biosensor, wherein the biosensor is useful for measuring analytes from a biological fluid, such as blood.
摘要翻译： 一组新的Os（II）和Os（III）化合物可用作电化学生物传感器中的氧化还原介质。 这些化合物具有1）低氧化电位，2）酶的电活性中心和化合物之间的快速反应动力学，3）氧的锇缓慢氧化，4）在水性介质中优异的溶解度。 这些介体特别可用作电化学生物传感器中使用的试剂的组分，其中生物传感器可用于测量生物流体如血液中的分析物。
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公开(公告)号：US5589326A
公开(公告)日：1996-12-31
申请号：US180492
申请日：1993-12-30
申请人： Zhi D. Deng , Gerald F. Sigler , Nigel A. Surridge , Christopher D. Wilsey , Robert J. McEnroe , Walter W. Jernigan , Rebecca W. Muddiman
发明人： Zhi D. Deng , Gerald F. Sigler , Nigel A. Surridge , Christopher D. Wilsey , Robert J. McEnroe , Walter W. Jernigan , Rebecca W. Muddiman
CPC分类号： C07F15/0026 , C12Q1/004 , Y10S435/817
摘要： A new group of Os(II) and Os(III) compounds useful as redox mediators in electrochemical biosensors. These compounds have 1) low oxidation potential, 2) fast reaction kinetics between the electroactive center of an enzyme and the compound, 3) slow oxidation of osmium by oxygen, and 4) excellent solubility in aqueous medium. These mediators are particularly useful as a component of a reagent used in an electrochemical biosensor, wherein the biosensor is useful for measuring analytes from a biological fluid, such as blood.
摘要翻译： 一组新的Os（II）和Os（III）化合物可用作电化学生物传感器中的氧化还原介质。 这些化合物具有1）低氧化电位，2）酶的电活性中心和化合物之间的快速反应动力学，3）氧的锇缓慢氧化，4）在水性介质中优异的溶解度。 这些介体特别可用作电化学生物传感器中使用的试剂的组分，其中生物传感器可用于测量生物流体如血液中的分析物。
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公开(公告)号：US4784945A
公开(公告)日：1988-11-15
申请号：US925720
申请日：1986-10-30
CPC分类号： C12Q1/60 , Y10S435/803 , Y10S436/907
摘要： A method for determining lipids using thin layer chromatography and multiple enzymatic staining is described. The method uses a hydrolase enzyme, an oxidase enzyme and a peroxidase enzyme in the presence of a chromogen precursor to form an oxidized chromogen stain. The method is particularly useful in determining phospholipids from animal and human tissue and/or fluids.
摘要翻译： 描述了使用薄层色谱法和多次酶染色测定脂质的方法。 该方法在色原体前体存在下使用水解酶，氧化酶和过氧化物酶形成氧化色原体染色剂。 该方法特别可用于从动物和人体组织和/或流体测定磷脂。
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