公开(公告)号：US20120178638A1

公开(公告)日：2012-07-12

申请号：US13061022

申请日：2009-09-04

申请人： Masad Damha ,  Jeremy Lackey ,  Debbie Mitra ,  Marvin Wickens ,  Franco Cerrina ,  Saima A. Akhtar ,  Mark Somoza

发明人： Masad Damha ,  Jeremy Lackey ,  Debbie Mitra ,  Marvin Wickens ,  Franco Cerrina ,  Saima A. Akhtar ,  Mark Somoza

IPC分类号： C40B30/04 ,  C07H21/02 ,  C40B40/06 ,  C07H19/10 ,  C07H19/20

CPC分类号： C07H21/02 ,  C07H19/067 ,  C07H19/167 ,  C12Q1/6806 ,  C12Q1/6837 ,  Y02P20/55

摘要： The present invention is directed to RNA monomers comprising O-acetal levulinyl protecting groups at the 2′ and/or the 5′-hydroxy functionalities of the ribose moiety. Said monomers may be incorporated into oligoribonucleotides or RNA polynucleotides. Furthermore, the invention is directed to methods for the synthesis of said RNA monomers, oligoribonucleotides and RNA polynucleotides, as well as methods for their deprotection and methods for the use of said compounds and compositions comprising said compounds. In particular, such compounds and compositions comprising them are used in methods for light-directed synthesis of RNA microarrays.

摘要翻译： 本发明涉及在核糖部分的2'和/或5'-羟基官能团上含有O-缩醛酰乙酰基保护基团的RNA单体。 所述单体可以掺入寡核糖核苷酸或RNA多核苷酸。 此外，本发明涉及用于合成所述RNA单体，寡核糖核苷酸和RNA多核苷酸的方法，以及其脱保护方法及使用所述化合物的方法和包含所述化合物的组合物。 特别地，包含它们的这些化合物和组合物用于RNA微阵列的光定向合成的方法中。

公开(公告)号：US09249175B2

公开(公告)日：2016-02-02

申请号：US13061022

申请日：2009-09-04

申请人： Masad Damha ,  Jeremy Lackey ,  Debbie Mitra ,  Marvin Wickens ,  Franco Cerrina ,  Saima A. Akhtar ,  Mark Somoza

发明人： Masad Damha ,  Jeremy Lackey ,  Debbie Mitra ,  Marvin Wickens ,  Franco Cerrina ,  Mark Somoza

IPC分类号： C40B30/04 ,  C07H21/02 ,  C40B40/06 ,  C07H19/10 ,  C07H19/20 ,  C07H19/067 ,  C07H19/167

CPC分类号： C07H21/02 ,  C07H19/067 ,  C07H19/167 ,  C12Q1/6806 ,  C12Q1/6837 ,  Y02P20/55

摘要： The present invention is directed to RNA monomers comprising O-acetal levulinyl protecting groups at the 2′ and/or the 5′-hydroxy functionalities of the ribose moiety. Said monomers may be incorporated into oligoribonucleotides or RNA polynucleotides. Furthermore, the invention is directed to methods for the synthesis of said RNA monomers, oligoribonucleotides and RNA polynucleotides, as well as methods for their deprotection and methods for the use of said compounds and compositions comprising said compounds. In particular, such compounds and compositions comprising them are used in methods for light-directed synthesis of RNA microarrays.

摘要翻译： 本发明涉及在核糖部分的2'和/或5'-羟基官能团上含有O-缩醛酰乙酰基保护基团的RNA单体。 所述单体可以掺入寡核糖核苷酸或RNA多核苷酸。 此外，本发明涉及用于合成所述RNA单体，寡核糖核苷酸和RNA多核苷酸的方法，以及其脱保护方法及使用所述化合物的方法和包含所述化合物的组合物。 特别地，包含它们的这些化合物和组合物用于RNA微阵列的光定向合成的方法中。