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公开(公告)号：US4419445A
公开(公告)日：1983-12-06
申请号：US360723
申请日：1982-03-22
CPC分类号： C12Q1/46 , C12Q2334/00
摘要： A method for determining the activity of cholinesterase comprising the steps of:mixing a solution containing p-methoxybenzoate demethylmonooxygenase, nicotinamide adenine dinucleotide reduced form (NADH) and p-methoxybenzoyl choline cation having the following formula: ##STR1## with a liquid containing cholinesterase, measuring the decrease in absorbance of light by NADH, which is based on a consumption of NADH, and calculating the activity of cholinesterase therefrom.
摘要翻译： 一种确定胆碱酯酶活性的方法，包括以下步骤：将含有对甲氧基苯甲酸甲酯脱甲基单加氧酶，烟酰胺腺嘌呤二核苷酸还原形式（NADH）和具有下式的对甲氧基苯甲酰胆碱阳离子的溶液与含有胆碱酯酶的液体混合， 测量基于NADH消耗的NADH对光的吸光度的降低，并计算其中胆碱酯酶的活性。
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公开(公告)号：US4432896A
公开(公告)日：1984-02-21
申请号：US423494
申请日：1982-09-24
IPC分类号： C12N9/48 , C07K1/113 , C07K5/06 , C12Q1/37 , C07C103/52
CPC分类号： C07K5/06026 , C12Q1/37
摘要： Derivatives of hippuryl-L-phenylalanine having the following general formula: ##STR1## wherein X represents OH or CH.sub.3 O useful as substrates for measuring the actively of carboxy peptidose A.
摘要翻译： 具有以下通式的马尿酰-L-苯丙氨酸的衍生物：其中X表示可用作测量羧基肽酶A活性的底物的OH或CH 3 O.
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3.发明授权Color developing method, enzyme immunoassay using the color developing method, and immunochromatography incorporating the enzyme immunoassay 失效彩色显影法，使用彩色显影法的酶免疫测定法和含有酶免疫测定法的免疫层析法
公开(公告)号：US5998156A
公开(公告)日：1999-12-07
申请号：US104166
申请日：1998-06-25
IPC分类号： G01N33/58 , G01N33/543 , G01N33/576
CPC分类号： G01N33/581 , Y10S435/962
摘要： Disclosed are a color developing method utilizing the reaction of an indolyl derivative with an enzyme in the presence of a specific free radical compound and/or a specific chelate compound, and an enzyme immunoassay using the color developing method.
摘要翻译： 公开了利用吲哚基衍生物与酶在特定自由基化合物和/或特定螯合物的存在下的反应的彩色显影方法，以及使用彩色显影法的酶免疫测定法。
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4.发明授权Color developing method, enzyme immunoassay using the color developing method, and immunochromatography incorporating the enzyme immunoassay 有权彩色显影法，使用彩色显影法的酶免疫测定法和含有酶免疫测定法的免疫层析法公开(公告)号：US06251618B1
公开(公告)日：2001-06-26
申请号：US09417220
申请日：1999-10-13
IPC分类号： G01N33543
CPC分类号： G01N33/581 , Y10S435/962
摘要： Disclosed are a color developing method utilizing the reaction of an indolyl derivative with an enzyme in the presence of a specific free radical compound and/or a specific chelate compound, and an enzyme immunoassay using the color developing method.
摘要翻译： 公开了利用吲哚基衍生物与酶在特定自由基化合物和/或特定螯合物的存在下的反应的彩色显影方法，以及使用彩色显影法的酶免疫测定法。
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公开(公告)号：US4634763A
公开(公告)日：1987-01-06
申请号：US428918
申请日：1982-09-30
申请人： Masami Sugiyama , Yasushi Kasahara
发明人： Masami Sugiyama , Yasushi Kasahara
CPC分类号： C12Q1/40
摘要： A novel amylase inhibitor, obtained from hard wheat and durum wheat by using dye ligand affinity chromatography having an inhibitory activity on human salivary amylase of less than 0.1 of the inhibitory activity on human pancreatic amylase is disclosed which is useful for measuring isoenzymes of amylase in the field of clinical tests.
摘要翻译： 公开了一种通过使用对人唾液淀粉酶具有抑制活性的染色配体亲和层析从硬质小麦和硬质小麦获得的新型淀粉酶抑制剂，其对人胰淀粉酶的抑制活性小于0.1，可用于测定淀粉酶的同工酶 临床试验领域。
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6.发明授权Derivatives of dipeptide and their use in determining the activity of carboxypeptidase A 失效二肽衍生物及其在测定羧肽酶A活性中的应用
公开(公告)号：US4551272A
公开(公告)日：1985-11-05
申请号：US568849
申请日：1984-01-06
CPC分类号： C07K5/06034 , C07K5/06026 , C07K5/06078
摘要： Derivatives of dipeptide having the following general formula and their use as substrates for colorimetrically or spectrophotometrically measuring the activity of carboxypeptidase A: ##STR1## wherein X is OH in ortho or para position, or NH.sub.2 or CH.sub.3 O in para position, Y is ##STR2##
摘要翻译： 具有以下通式的二肽衍生物及其用作比色或分光光度测量羧肽酶A的活性的底物：其中X是邻位或对位的OH，或对位的NH 2或CH 3 O，Y是
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公开(公告)号：US07803638B2
公开(公告)日：2010-09-28
申请号：US11379617
申请日：2006-04-21
申请人： Masami Sugiyama
发明人： Masami Sugiyama
IPC分类号： G01N33/533 , G01N33/53 , G01N21/76
CPC分类号： G01N33/582 , Y10S436/80 , Y10T436/17 , Y10T436/18
摘要： The present invention relates to a fluorescent label characterized by containing a hydrophilic polymer having an anionic group, a polyether derivative, and a phosphor, in which the phosphor is bound to the hydrophilic polymer via the polyether derivative, and also relates to a fluorescently labeled recognition substance labeled with the fluorescent label, and an immunoassay method using the recognition substance.
摘要翻译： 本发明涉及一种荧光标记，其特征在于含有具有阴离子基团的亲水性聚合物，聚醚衍生物和荧光体，其中荧光体通过聚醚衍生物与亲水性聚合物结合，并且还涉及荧光标记的识别 用荧光标记标记的物质和使用识别物质的免疫测定法。
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公开(公告)号：US20060240479A1
公开(公告)日：2006-10-26
申请号：US11379617
申请日：2006-04-21
申请人： Masami Sugiyama
发明人： Masami Sugiyama
CPC分类号： G01N33/582 , Y10S436/80 , Y10T436/17 , Y10T436/18
摘要： The present invention relates to a fluorescent label characterized by containing a hydrophilic polymer having an anionic group, a polyether derivative, and a phosphor, in which the phosphor is bound to the hydrophilic polymer via the polyether derivative, and also relates to a fluorescently labeled recognition substance labeled with the fluorescent label, and an immunoassay method using the recognition substance.
摘要翻译： 本发明涉及一种荧光标记，其特征在于含有具有阴离子基团的亲水性聚合物，聚醚衍生物和荧光体，其中荧光体通过聚醚衍生物与亲水性聚合物结合，并且还涉及荧光标记的识别 用荧光标记标记的物质和使用识别物质的免疫测定法。
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公开(公告)号：US5432609A
公开(公告)日：1995-07-11
申请号：US177843
申请日：1994-01-05
申请人： Masami Sugiyama , Takao Sakai
发明人： Masami Sugiyama , Takao Sakai
摘要： A two-dimensional colorimeter focuses light through a beam splitter onto a rotary filter so that a first light receiving element can receive light from multiple points on a sample. A rotating filter can provide multiple measurements based upon different wavelengths of light. A portion of the light from the beam splitter also contacts a spectral sensor which separates the light from a particular point into primary wavelength elements which are converted into electrical signals. Based upon these primary wavelength elements, the first signals from the light receiving element are corrected. As a result, the measurement of chromaticity of multiple points on a sample can be performed accurately using a device of simple construction.
摘要翻译： 二维色度计将光束通过分束器聚焦到旋转滤光器上，使得第一光接收元件可以接收来自样品上多个点的光。 旋转滤波器可以基于不同波长的光提供多个测量。 来自分束器的光的一部分还接触光谱传感器，光谱传感器将光从特定点分离成主要波长元件，其被转换成电信号。 基于这些主波长元件，来自光接收元件的第一信号被校正。 结果，可以使用简单结构的装置来精确地进行样品上多个点的色度测量。
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公开(公告)号：US4205918A
公开(公告)日：1980-06-03
申请号：US881177
申请日：1978-02-27
申请人： Tohru Kisanuki , Masami Sugiyama , Takeshi Kosaka
发明人： Tohru Kisanuki , Masami Sugiyama , Takeshi Kosaka
摘要： A hand-carriable, compact color meter for indicating proper amount of adjustment of color compensation filters interposed in the light path of a color printer for the compensation of color balance of the light illuminating a film or the slide set on the printer. The color meter includes an indicating lamp, a photocell to be exposed to the light passing through the film or slide, a plurality of optical color filters selectively disposed in front of the photocell to provide the photocell with spectral sensitivities corresponding to the spectral sensitivity of the printing paper, a plurality of fixed or variable resistors selectable in correspondence to the selection of the color filter, and an electric circuit for actuating the indicating lamp in accordance with the relationship between the output of the light measuring circuit and a reference voltage commensurate with the selected one of the resistors. In a preferred embodiment, the color meter further includes a combination of a variable resistor and a filter for determination of exposure time.
摘要翻译： 一种手提式紧凑型色表，用于指示插入彩色打印机光路中的适当量的色彩补偿滤光片的调整，以补偿照亮胶片或设置在打印机上的幻灯片的光的色彩平衡。 色度计包括指示灯，要暴露于穿过膜或载玻片的光的光电池，选择性地设置在光电管前面的多个光学滤色器，以向光电池提供与光电池的光谱灵敏度相对应的光谱灵敏度 打印纸，对应于滤色器的选择可选择的多个固定或可变电阻器，以及用于根据光测量电路的输出与与该测量电路相当的参考电压之间的关系致动指示灯的电路 选择一个电阻。 在优选实施例中，色度计还包括可变电阻器和用于确定曝光时间的滤光器的组合。
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