公开(公告)号：US08163543B2

公开(公告)日：2012-04-24

申请号：US12091022

申请日：2006-10-19

申请人： Masashi Urabe ,  Keiya Ozawa ,  Saskia Jacoba Petronella Haast ,  Wilhelmus Theodorus Johannes Maria Christiaan Hermens

发明人： Masashi Urabe ,  Keiya Ozawa ,  Saskia Jacoba Petronella Haast ,  Wilhelmus Theodorus Johannes Maria Christiaan Hermens

IPC分类号： C12N15/33 ,  C12N15/07 ,  C12N7/01

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14144 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14151

摘要： The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon. The insect cell further comprises a second nucleotide sequence comprising at least one AAV inverted terminal repeat (ITR) nucleotide sequence; a third nucleotide sequence comprising a Rep52 or a Rep40 coding sequence operably linked to expression control sequences for expression in an insect cell; and, a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence operably linked to expression control sequences for expression in an insect cell. The invention further relates to adeno-associated viral vectors with an altered ratio of the viral capsid proteins that provides improved infectivity of the viral particles.

摘要翻译： 本发明涉及在昆虫细胞中产生腺相关病毒载体。 昆虫细胞因此包含编码腺相关病毒（AAV）衣壳蛋白的第一核苷酸序列，由此AAV VP1衣壳蛋白翻译的起始密码子是非ATG，次最初始密码子。 昆虫细胞还包含第二核苷酸序列，其包含至少一个AAV反向末端重复（ITR）核苷酸序列; 包含可操作地连接到用于在昆虫细胞中表达的表达控制序列的Rep52或Rep40编码序列的第三核苷酸序列; 以及包含与表达控制序列可操作地连接以在昆虫细胞中表达的Rep78或Rep68编码序列的第四核苷酸序列。 本发明还涉及具有改变的病毒衣壳蛋白比例提高病毒颗粒感染性的腺相关病毒载体。

公开(公告)号：US20120028357A1

公开(公告)日：2012-02-02

申请号：US12091022

申请日：2006-10-19

申请人： Masashi Urabe ,  Keiya Ozawa ,  Saskia Jacoba Petronella Haast ,  Wilhelmus Theodorus Johannes Hermens

发明人： Masashi Urabe ,  Keiya Ozawa ,  Saskia Jacoba Petronella Haast ,  Wilhelmus Theodorus Johannes Hermens

IPC分类号： C12N15/66 ,  C12N15/35 ,  C12N7/00 ,  C12N15/00 ,  C12N15/864

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14144 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14151

摘要： The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon. The insect cell further comprises a second nucleotide sequence comprising at least one AAV inverted terminal repeat (ITR) nucleotide sequence; a third nucleotide sequence comprising a Rep52 or a Rep40 coding sequence operably linked to expression control sequences for expression in an insect cell; and, a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence operably linked to expression control sequences for expression in an insect cell. The invention further relates to adeno-associated viral vectors with an altered ratio of the viral capsid proteins that provides improved infectivity of the viral particles.

摘要翻译： 本发明涉及在昆虫细胞中产生腺相关病毒载体。 因此，昆虫细胞包含编码腺相关病毒（AAV）衣壳蛋白的第一核苷酸序列，其中AAV VP1衣壳蛋白的翻译起始密码子是非ATG，次最初始密码子。 昆虫细胞还包含第二核苷酸序列，其包含至少一个AAV反向末端重复（ITR）核苷酸序列; 包含可操作地连接到用于在昆虫细胞中表达的表达控制序列的Rep52或Rep40编码序列的第三核苷酸序列; 以及包含与表达控制序列可操作地连接以在昆虫细胞中表达的Rep78或Rep68编码序列的第四核苷酸序列。 本发明还涉及具有改变的病毒衣壳蛋白比例提高病毒颗粒感染性的腺相关病毒载体。

公开(公告)号：US06566981B2

公开(公告)日：2003-05-20

申请号：US09917594

申请日：2001-07-27

申请人： Masashi Urabe ,  Kenji Noguchi ,  Kouichi Maruta

发明人： Masashi Urabe ,  Kenji Noguchi ,  Kouichi Maruta

IPC分类号： H03H964

CPC分类号： H03H9/1071 ,  H03H9/6483

摘要： A surface acoustic wave device has: input external terminals, output external terminals and ground external terminals disposed on the outer peripheral surfaces of a container. In a cavity portion of the container, input pads, output pads and ground pads are respectively electrically connected to the input external terminals, the output external terminals and the ground external terminals. A plurality of SAW filter elements are housed in the cavity portion. A plurality of ground conductor films are respectively formed in areas where the SAW filter elements are respectively housed. The input, output and ground electrodes of the SAW filter elements are respectively connected to the input, the output and the ground pads. A metallic lid hermetically seals the cavity portion. Only one SAW filter element is connected to the same ground pad. All the round conductor films on which the SAW Liter elements are mounted are electrically separated from one another.

摘要翻译： 表面声波装置具有：输入外部端子，输出外部端子和设置在容器的外周面上的接地外部端子。 在容器的空腔部分中，输入焊盘，输出焊盘和接地焊盘分别电连接到输入外部端子，输出外部端子和接地外部端子。 多个SAW滤波器元件容纳在空腔部分中。 多个接地导体膜分别形成在SAW滤波器元件分别容纳的区域中。 SAW滤波器元件的输入，输出和接地电极分别连接到输入，输出和接地焊盘。 金属盖密封空腔部分。 只有一个SAW滤波器元件连接到同一个接地垫。 其上安装有SAW放置元件的所有圆形导体膜彼此电分离。

公开(公告)号：US07271002B2

公开(公告)日：2007-09-18

申请号：US10415834

申请日：2002-11-08

申请人： Robert M. Kotin ,  Masashi Urabe ,  Chuan-Tian Ding

发明人： Robert M. Kotin ,  Masashi Urabe ,  Chuan-Tian Ding

IPC分类号： C12N15/35 ,  C12N15/63 ,  C12N15/64 ,  C12N15/864 ,  C12N5/10 ,  A61K48/00

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2830/00 ,  C12N2830/60 ,  C12N2840/44

摘要： A method of producing an adeno-associated virus (AAV) in an insect cell comprising (i) providing at least one insect cell-compatible vector comprising a first nucleotide sequence comprising at least one AAV ITR nucleotide sequence, a second nucleotide sequence containing an open reading frame encoding AAV VP1, VP2, and VP3 capsid proteins, a third nucleotide sequence comprising a Rep52 or a Rep40 coding sequence, and a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence, (ii) introducing the at least one insect cell-compatible vector into an insect cell, and (iii) maintaining the insect cell under conditions such that AAV is produced. Also provided are recombinant AAV made in accordance with the method, insect cell-compatible vectors, and insect cells comprising nucleotide sequences for production of AAV in an insect cell.

摘要翻译： 一种在昆虫细胞中产生腺相关病毒（AAV）的方法，包括：（i）提供至少一种昆虫细胞相容性载体，其包含含有至少一个AAV ITR核苷酸序列的第一核苷酸序列， 编码AAV VP1，VP2和VP3衣壳蛋白的阅读框，包含Rep52或Rep40编码序列的第三核苷酸序列，以及包含Rep78或Rep68编码序列的第四核苷酸序列，（ii）引入至少一种昆虫细胞 并且（iii）在产生AAV的条件下维持昆虫细胞。 还提供了根据该方法制备的重组AAV，昆虫细胞相容载体和包含用于在昆虫细胞中产生AAV的核苷酸序列的昆虫细胞。

公开(公告)号：US06723551B2

公开(公告)日：2004-04-20

申请号：US10216870

申请日：2002-08-13

申请人： Robert M. Kotin ,  Masashi Urabe ,  Chuan-Tian Ding

发明人： Robert M. Kotin ,  Masashi Urabe ,  Chuan-Tian Ding

IPC分类号： C12N700

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N2710/16143 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2830/00 ,  C12N2830/60 ,  C12N2840/20

摘要： A method of producing an adeno-associated virus (AAV) in an insect cell comprising (i) providing at least one insect cell-compatible vector comprising a first nucleotide sequence comprising at least one AAV ITR nucleotide sequence, a second nucleotide sequence containing an open reading frame encoding AAV VP1, VP2, and VP3 capsid proteins, a third nucleotide sequence comprising a Rep52 or a Rep40 coding sequence, and a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence, (ii) introducing the at least one insect cell-compatible vector into an insect cell, and (iii) maintaining the insect cell under conditions such that AAV is produced. Also provided are recombinant AAV made in accordance with the method, insect cell-compatible vectors, and insect cells comprising nucleotide sequences for production of AAV in an insect cell.

摘要翻译： 一种在昆虫细胞中产生腺相关病毒（AAV）的方法，包括：（i）提供至少一种昆虫细胞相容性载体，其包含含有至少一个AAV ITR核苷酸序列的第一核苷酸序列， 编码AAV VP1，VP2和VP3衣壳蛋白的阅读框，包含Rep52或Rep40编码序列的第三核苷酸序列，以及包含Rep78或Rep68编码序列的第四核苷酸序列，（ii）引入至少一种昆虫细胞 并且（iii）在产生AAV的条件下维持昆虫细胞。 还提供了根据该方法制备的重组AAV，昆虫细胞相容载体和包含用于在昆虫细胞中产生AAV的核苷酸序列的昆虫细胞。